| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,205,451 | A→T | 100% | V17V (GTT→GTA) | yoaE ← | fused predicted membrane proteins |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,205,451 | 0 | A | T | 80.4% | 101.0 / 20.9 | 46 | V17V (GTT→GTA) | yoaE | fused predicted membrane proteins |
| Reads supporting (aligned to +/- strand): ref base A (9/0); new base T (0/37); total (9/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.08e-10 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.83e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ATACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGACAAGCTCCTGCTT > minE/1205428‑1205516 | aTACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGa > 1:219501/1‑67 (MQ=255)aTACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGa > 1:775442/1‑67 (MQ=255)aTACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGa > 1:2221956/1‑67 (MQ=255)aTACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGa > 1:31019/1‑67 (MQ=255)aTACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGa > 1:1529188/1‑67 (MQ=255)aTACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGa > 1:681498/1‑67 (MQ=255)aTACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGa > 1:637637/1‑67 (MQ=255)aTACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGa > 1:1502618/1‑67 (MQ=255)aTACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCACGCCCAAATTGAGGGGTCCATTAAg > 1:385694/1‑66 (MQ=255) acaTTTTCGAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa < 1:1066259/67‑1 (MQ=255) acaATTTCTAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa < 1:2007807/67‑1 (MQ=255) acaATTTCGAGAACTACAAGCGTGAGTAGGCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa < 1:900431/67‑1 (MQ=255) acaATTTCGAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGTATTCCATGa < 1:576614/67‑1 (MQ=255) acaATTTCGAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCTATGa < 1:3929/67‑1 (MQ=255) acaATTTCGAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa < 1:104414/67‑1 (MQ=255) acaATTTCGAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa < 1:478081/67‑1 (MQ=255) acaATTTCGAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa < 1:370438/67‑1 (MQ=255) acaATTTCGAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa < 1:712942/67‑1 (MQ=255) acaATTTCGAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa < 1:315158/67‑1 (MQ=255) acaATTTCGAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa < 1:725613/67‑1 (MQ=255) acaATTTCGAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa < 1:308879/67‑1 (MQ=255) acaATTTCGAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa < 1:2304014/67‑1 (MQ=255) acaATTTCGAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa < 1:2271109/67‑1 (MQ=255) acaATTTCGAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa < 1:732834/67‑1 (MQ=255) acaATTTCGAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa < 1:812069/67‑1 (MQ=255) acaATTTCGAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa < 1:979969/67‑1 (MQ=255) acaATTTCGAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa < 1:1395534/67‑1 (MQ=255) acaATTTCGAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa < 1:1036292/67‑1 (MQ=255) acaATTTCGAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa < 1:1062389/67‑1 (MQ=255) acaATTTCGAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa < 1:110663/67‑1 (MQ=255) acaATTTCGAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa < 1:1106745/67‑1 (MQ=255) acaATTTCGAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa < 1:1112338/67‑1 (MQ=255) acaATTTCGAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa < 1:1215492/67‑1 (MQ=255) acaATTTCGAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa < 1:1292487/67‑1 (MQ=255) acaATTTCGAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa < 1:13922/67‑1 (MQ=255) acaATTTCGAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa < 1:2018641/67‑1 (MQ=255) acaATTTCGAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa < 1:1481581/67‑1 (MQ=255) acaATTTCGAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa < 1:1532020/67‑1 (MQ=255) acaATTTCGAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa < 1:1625434/67‑1 (MQ=255) acaATTTCGAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa < 1:1745849/67‑1 (MQ=255) acaATTTCGAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa < 1:1765021/67‑1 (MQ=255) acaATTTCGAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa < 1:1799807/67‑1 (MQ=255) acaATTTCGAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa < 1:1829967/67‑1 (MQ=255) cattttCGAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa < 1:1298449/63‑1 (MQ=255) caATTTCGAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa < 1:1503797/66‑1 (MQ=255) caATTTCGAGAACTACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa < 1:2054415/66‑1 (MQ=255) caacaaGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGACaa < 1:1189132/57‑1 (MQ=255) caacaaGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGACaa < 1:201583/57‑1 (MQ=255) caacaaGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGACaa < 1:898731/57‑1 (MQ=255) caacaaGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGACaa < 1:1051645/57‑1 (MQ=255) caacaaGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGACAAGCTCCTGCtt < 1:2115140/67‑1 (MQ=255) | ATACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGACAAGCTCCTGCTT > minE/1205428‑1205516 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |