| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,345,150 | C→A | 100% | D234Y (GAC→TAC) | wza ← | lipoprotein required for capsular polysaccharide translocation through the outer membrane |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,345,150 | 0 | C | A | 83.8% | 79.2 / 14.4 | 37 | D234Y (GAC→TAC) | wza | lipoprotein required for capsular polysaccharide translocation through the outer membrane |
| Reads supporting (aligned to +/- strand): ref base C (2/4); new base A (0/31); total (2/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.25e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ATCATTACGTGGCACGTAGAGAATATCGCCGGGGTAAAGCAGGCGATTCTGGTTGAGGTCGCCGTTTTGCATCAGCGCCTGCAAAGAAATGCGCTCTTCACGACC > minE/1345091‑1345195 | atcatTACGTGGCACGTAGAGAATATCGCCGGGGTCAAGCAGGCGATTCTGGTTGAGGTAGCCGttt < 1:874284/67‑1 (MQ=255)atcatTACGTGGCACGTAGAGAATATCGCCGGGGTAAAGCAGGCGTTTCTGGTTGAGGTAGCCGttt < 1:506680/67‑1 (MQ=255)atcatTACGTGGCACGTAGAGAATATCGCCGGGGTAAAGCAGGCGATTCTGGTTGAGGTAGCCGttt < 1:1090453/67‑1 (MQ=255)atcatTACGTGGCACGTAGAGAATATCGCCGGGGTAAAGCAGGCGATTCTGGTTGAGGTAGCCGttt < 1:593677/67‑1 (MQ=255)atcatTACGTGGCACGTAGAGAATATCGCCGGGGTAAAGCAGGCGATTCTGGTTGAGGTAGCCGttt < 1:495308/67‑1 (MQ=255)atcatTACGTGGCACGTAGAGAATATCGCCGGGGTAAAGCAGGCGATTCTGGTTGAGGTAGCCGttt < 1:481132/67‑1 (MQ=255)atcatTACGTGGCACGTAGAGAATATCGCCGGGGTAAAGCAGGCGATTCTGGTTGAGGTAGCCGttt < 1:476623/67‑1 (MQ=255)atcatTACGTGGCACGTAGAGAATATCGCCGGGGTAAAGCAGGCGATTCTGGTTGAGGTAGCCGttt < 1:441089/67‑1 (MQ=255)atcatTACGTGGCACGTAGAGAATATCGCCGGGGTAAAGCAGGCGATTCTGGTTGAGGTAGCCGttt < 1:393848/67‑1 (MQ=255)atcatTACGTGGCACGTAGAGAATATCGCCGGGGTAAAGCAGGCGATTCTGGTTGAGGTAGCCGttt < 1:320363/67‑1 (MQ=255)atcatTACGTGGCACGTAGAGAATATCGCCGGGGTAAAGCAGGCGATTCTGGTTGAGGTAGCCGttt < 1:66381/67‑1 (MQ=255)atcatTACGTGGCACGTAGAGAATATCGCCGGGGTAAAGCAGGCGATTCTGGTTGAGGTAGCCGttt < 1:2135058/67‑1 (MQ=255)atcatTACGTGGCACGTAGAGAATATCGCCGGGGTAAAGCAGGCGATTCTGGTTGAGGTAGCCGttt < 1:1045402/67‑1 (MQ=255)atcatTACGTGGCACGTAGAGAATATCGCCGGGGTAAAGCAGGCGATTCTGGTTGAGGTAGCCGttt < 1:843117/67‑1 (MQ=255)atcatTACGTGGCACGTAGAGAATATCGCCGGGGTAAAGCAGGCGATTCTGGTTGAGGTAGCCGttt < 1:1786517/67‑1 (MQ=255)atcatTACGTGGCACGTAGAGAATATCGCCGGGGTAAAGCAGGCGATTCTGGTTGAGGTAGCCGttt < 1:1639750/67‑1 (MQ=255)atcatTACGTGGCACGTAGAGAATATCGCCGGGGTAAAGCAGGCGATTCTGGTTGAGGTAGCCGttt < 1:1553787/67‑1 (MQ=255)atcatTACGTGGCACGTAGAGAATATCGCCGGGGTAAAGCAGGCGATTCTGGTTGAGGTAGCCGttt < 1:1425174/67‑1 (MQ=255)atcatTACGTGGCACGTAGAGAATATCGCCGGGGTAAAGCAGGCGATTCTGGTTGAGGTAGCCGttt < 1:878464/67‑1 (MQ=255)atcatTACGTGGCACGTAGAGAATATCGCCGGGGTAAAGCAGGCGATTCTGGTTGAGGTAGCCGttt < 1:1283260/67‑1 (MQ=255)atcatTACGTGGCACGTAGAGAATATCGCCGGGGTAAAGCAGGCGATTCTGGTTGAGGTAGCCGttt < 1:1252410/67‑1 (MQ=255)atcatTACGTGGCACGTAGAGAATATCGCCGGGGTAAAGCAGGCGATTCTGGTTGAGGTAGCCGttt < 1:1232529/67‑1 (MQ=255)atcatTACGTGGCACGTAGAGAATATCGCCGGGGTAAAGCAGGCGATTCTGGTTGAGGTAGCCGttt < 1:1186807/67‑1 (MQ=255)atcatTACGTGGCACGTAGAGAATATCGCCGGGGTAAAGCAGGCGATTCTGGTTGAGGTAGCCGttt < 1:932212/67‑1 (MQ=255)atcatTACGTGGCACGTAGAGAATATCGCCGGGGTAAAGCAGGCGATTCTGGTTGAGGTAGCCGttt < 1:111755/67‑1 (MQ=255) tcatTACGTGGCACGTAGAGAATATCGCCGGGGTAAAGCAGGCGATTCTGGTTGAGGTAGCGGttt < 1:157/66‑1 (MQ=255) tcatTACGTGGCACGTAGAGAATATCGCCGGGGTAAAGCAGGCGATTCTGGTTGAGGTAGCCGttt < 1:1740340/66‑1 (MQ=255) tcatTACGTGGCACGTAGAGAATATCGCCGGGGTAAAGCAGGCGATTCTGGTTGAGGTAGCCGttt < 1:854334/66‑1 (MQ=255) tACGTGGCACGTAGAGAATATCGCCGGGGTAAAGCAGGCGATTCTGGTTGAGGTAGCCGttt < 1:2114644/62‑1 (MQ=255) aCGTGGCACGTAGAGAATATCGCCGGGGTAAAGCAGGCGATTCTGGTTGAGGTAGCCGttt < 1:948318/61‑1 (MQ=255) aCGTAGAGAATATCGCCGGGGTAAAGCAGGCGATTCTGGTTGAGGTCGCCGTTTTGCATCAGCGcc > 1:2206504/1‑66 (MQ=255) aCGTAGAGAATATCGCCGGGGTAAAGCAGGCGATTCTGGTTGAGGTCGCCGTTTTGCATCAGCGcc > 1:1178246/1‑66 (MQ=255) cGCCGGGGTAAAGCAGGCGATTCTGGTTGAGGTAGCCGttt < 1:1315687/41‑1 (MQ=255) cTGGTTGAGGTCGCCGTTTTGCATCAGCGCCTGCAAAGAAATGCGCTCTTc < 1:2014943/51‑1 (MQ=255) cTGGTTGAGGTCGCCGTTTTGCATCAGCGCCTGCAAAGAAATGCGCTCTTc < 1:1855199/51‑1 (MQ=255) cTGGTTGAGGTCGCCGTTTTGCATCAGCGCCTGCAAAGAAATGCGCTCTTc < 1:1291421/51‑1 (MQ=255) tGAGGTCGCCGTTTTGCATCAGCGCCTGCAAAGAAATGCGCTCTTCACGAcc < 1:514516/52‑1 (MQ=255) | ATCATTACGTGGCACGTAGAGAATATCGCCGGGGTAAAGCAGGCGATTCTGGTTGAGGTCGCCGTTTTGCATCAGCGCCTGCAAAGAAATGCGCTCTTCACGACC > minE/1345091‑1345195 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |