| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,418,642 | T→C | 100% | N197N (AAT→AAC) | narP → | DNA‑binding response regulator in two‑component regulatory system with NarQ or NarX |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,418,642 | 0 | T | C | 80.0% | 165.9 / 35.4 | 80 | N197N (AAT→AAC) | narP | DNA‑binding response regulator in two‑component regulatory system with NarQ or NarX |
| Reads supporting (aligned to +/- strand): ref base T (0/16); new base C (0/64); total (0/80) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TAAAAGTACATATTCGCAATCTGCTGCGTAAACTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAATA > minE/1418605‑1418698 | tAAAAGTCCATATTCGCAATCTGCTGCGTAAACTCGACGTCCGCTCACGCGTGGCGGCCACCATTCt < 1:1363550/67‑1 (MQ=255)tAAAAGTCCATATTCGCAATCTGCTGCGTAAACTCAACGTCCGCTCACGCGTGGCGGCCACCCTTCt < 1:5471/67‑1 (MQ=255)tAAAAGTCCATATTCGCAATCTGCTGCGTAAACTCAACGTCCGCTCACGCGTGGCGGCCACCATTCt < 1:2116924/67‑1 (MQ=255)tAAAAGTCCATATTCGCAATCTGCTGCGTAAACTCAACGTCCGCTCACGCGTGGCGGCCACCATTCt < 1:1758380/67‑1 (MQ=255)tAAAAGTCCATATTCGCAATCTGCTGCGTAAACTCAACGTCCGCTCACGCGTGGCGGCCACCATTCt < 1:668991/67‑1 (MQ=255)tAAAAGTCCATATTCGCAATCTGCTGCGTAAACTCAACGTCCGCTCACGCGTGGCGGCCACCATTCt < 1:1786769/67‑1 (MQ=255)tAAAAGTCCATATTCGCAATCTGCTGCGTAAACTCAACGTCCGCTCACGCGTGGCGGCCACCATTCt < 1:562657/67‑1 (MQ=255)tAAAAGTCCATATTCGCAATCTGCTGCGTAAACTCAACGTCCGCTCACGCGTGGCGGCCACCATTCt < 1:1907781/67‑1 (MQ=255)tAAAAGTCCATATTCGCAATCTGCTGCGTAAACTCAACGTCCGCTCACGCGTGGCGGCCACCATTCt < 1:1950812/67‑1 (MQ=255)tAAAAGTCCATATTCGCAATCTGCTGCGTAAACTCAACGTCCGCTCACGCGTGGCGGCCACCATTCt < 1:1988765/67‑1 (MQ=255)tAAAAGTCCATATTCGCAATCTGCTGCGTAAACTCAACGTCCGCTCACGCGTGGCGGCCACCATTCt < 1:2005932/67‑1 (MQ=255)tAAAAGTCCATATTCGCAATCTGCTGCGTAAACTCAACGTCCGCTCACGCGTGGCGGCCACCATTCt < 1:2030347/67‑1 (MQ=255)tAAAAGTCCATATTCGCAATCTGCTGCGTAAACTCAACGTCCGCTCACGCGTGGCGGCCACCATTCt < 1:104320/67‑1 (MQ=255)tAAAAGTCCATATTCGCAATCTGCTGCGTAAACTCAACGTCCGCTCACGCGTGGCGGCCACCATTCt < 1:2072748/67‑1 (MQ=255)tAAAAGTCCATATTCGCAATCTGCTGCGTAAACTCAACGTCCGCTCACGCGTGGCGGCCACCATTCt < 1:453828/67‑1 (MQ=255)tAAAAGTCCATATTCGCAATCTGCTGCGTAAACTCAACGTCCGCTCACGCGTGGCGGCCACCATTCt < 1:2083139/67‑1 (MQ=255)tAAAAGTCCATATTCGCAATCTGCTGCGTAAACTCAACGTCCGCTCACGCGTGGCGGCCACCATTCt < 1:699131/67‑1 (MQ=255)tAAAAGTCCATATTCGCAATCTGCTGCGTAAACTCAACGTCCGCTCACGCGTGGCGGCCACCATTCt < 1:421768/67‑1 (MQ=255)tAAAAGTCCATATTCGCAATCTGCTGCGTAAACTCAACGTCCGCTCACGCGTGGCGGCCACCATTCt < 1:2235924/67‑1 (MQ=255)tAAAAGTCCATATTCGCAATCTGCTGCGTAAACTCAACGTCCGCTCACGCGTGGCGGCCACCATTCt < 1:2241383/67‑1 (MQ=255)tAAAAGTCCATATTCGCAATCTGCTGCGTAAACTCAACGTCCGCTCACGCGTGGCGGCCACCATTCt < 1:2293433/67‑1 (MQ=255)tAAAAGTCCATATTCGCAATCTGCTGCGTAAACTCAACGTCCGCTCACGCGTGGCGGCCACCATTCt < 1:235960/67‑1 (MQ=255)tAAAAGTCCATATTCGCAATCTGCTGCGTAAACTCAACGTCCGCTCACGCGTGGCGGCCACCATTCt < 1:26655/67‑1 (MQ=255)tAAAAGTCCATATTCGCAATCTGCTGCGTAAACTCAACGTCCGCTCACGCGTGGCGGCCACCATTCt < 1:294723/67‑1 (MQ=255)tAAAAGTCCATATTCGCAATCTGCTGCGTAAACTCAACGTCCGCTCACGCGTGGCGGCCACCATTCt < 1:30379/67‑1 (MQ=255)tAAAAGTCCATATTCGCAATCTGCTGCGTAAACTCAACGTCCGCTCACGCGTGGCGGCCACCATTCt < 1:369075/67‑1 (MQ=255)tAAAAGTCCATATTCGCAATCTGCTGCGTAAACTCAACGTCCGCTCACGCGTGGCGGCCACCATTCt < 1:381661/67‑1 (MQ=255)tAAAAGTCCATATTCGCAATCTGCTGCGTAAACTCAACGTCCGCTCACGCGTGGCGGCCACCATTCt < 1:41799/67‑1 (MQ=255)tAAAAGTCCATATTCGCAATCTGCTGCGTAAACTCAACGTCCGCTCACGCGTGGCGGCCACCATTCt < 1:420400/67‑1 (MQ=255)tAAAAGTCCATATTCGCAATCTGCTGCGTAAACTCAACGTCCGCTCACGCGTGGCGGCCACCATTCt < 1:1479810/67‑1 (MQ=255)tAAAAGTCCATATTCGCAATCTGCTGCGTAAACTCAACGTCCGCTCACGCGTGGCGGCCACCATTCt < 1:110622/67‑1 (MQ=255)tAAAAGTCCATATTCGCAATCTGCTGCGTAAACTCAACGTCCGCTCACGCGTGGCGGCCACCATTCt < 1:932913/67‑1 (MQ=255)tAAAAGTCCATATTCGCAATCTGCTGCGTAAACTCAACGTCCGCTCACGCGTGGCGGCCACCATTCt < 1:845780/67‑1 (MQ=255)tAAAAGTCCATATTCGCAATCTGCTGCGTAAACTCAACGTCCGCTCACGCGTGGCGGCCACCATTCt < 1:11386/67‑1 (MQ=255)tAAAAGTCCATATTCGCAATCTGCTGCGTAAACTCAACGTCCGCTCACGCGTGGCGGCCACCATTCt < 1:1196004/67‑1 (MQ=255)tAAAAGTCCATATTCGCAATCTGCTGCGTAAACTCAACGTCCGCTCACGCGTGGCGGCCACCATTCt < 1:784275/67‑1 (MQ=255)tAAAAGTCCATATTCGCAATCTGCTGCGTAAACTCAACGTCCGCTCACGCGTGGCGGCCACCATTCt < 1:781316/67‑1 (MQ=255)tAAAAGTCCATATTCGCAATCTGCTGCGTAAACTCAACGTCCGCTCACGCGTGGCGGCCACCATTCt < 1:1349754/67‑1 (MQ=255)tAAAAGTCCATATTCGCAATCTGCTGCGTAAACTCAACGTCCGCTCACGCGTGGCGGCCACCATTCt < 1:1405400/67‑1 (MQ=255)tAAAAGTCCATATTCGCAATCTGCTGCGTAAACTCAACGTCCGCTCACGCGTGGCGGCCACCATTCt < 1:781191/67‑1 (MQ=255)tAAAAGTCCATATTCGCAATCTGCTGCGTAAACTCAACGTCCGCTCACGCGTGGCGGCCACCATTCt < 1:773584/67‑1 (MQ=255)tAAAAGTCCATATTCGCAATCTGCTGCGTAAACTCAACGTCCGCTCACGCGTGGCGGCCACCATTCt < 1:143067/67‑1 (MQ=255)tAAAAGTCCATATTCGCAATCTGCTGCGTAAACTCAACGTCCGCTCACGCGTGGCGGCCACCATTCt < 1:1440356/67‑1 (MQ=255)tAAAAGTCCATATTCGCAATCTGCTGCGTAAACTCAACGTCCGCTCACGCGTGGCGGCCACCATTCt < 1:1481342/67‑1 (MQ=255)tAAAAGTCCATATTCGCAATCTGCTGCGTAAACTCAACGTCCGCTCACGCGTGGCGGCCACCATTCt < 1:1512969/67‑1 (MQ=255)tAAAAGTCCATATTCGCAATCTGCTGCGTAAACTCAACGTCCGCTCACGCGTGGCGGCCACCATTCt < 1:765822/67‑1 (MQ=255)tAAAAGTCCATATTCGCAATCTGCTGCGTAAACTCAACGTCCGCTCACGCGTGGCGGCCACCATTCt < 1:1514927/67‑1 (MQ=255)tAAAAGTCCATATTCGCAATCTGCTGCGTAAACTCAACGTCCGCTCACGCGTGGCGGCCACCATTCt < 1:1519295/67‑1 (MQ=255)tAAAAGTCCATATTCGCAATCTGCTGCGTAAACTCAACGTCCGCTCACGCGTGGCGGCCACCATTCt < 1:1568587/67‑1 (MQ=255)tAAAAGTCCATATTCGCAATCTGCTGCGTAAACTCAACGTCCGCTCACGCGTGGCGGCCACCATTCt < 1:1574596/67‑1 (MQ=255)tAAAAGTCCATATTCGCAATCTGCTGCGTAAACTCAACGTCCGCTCACGCGTGGCGGCCACCATTCt < 1:765271/67‑1 (MQ=255)tAAAAGTCCATATTCGCAATCTGCTGCGTAAACTCAACGTCCGCTCACGCGTGGCGGCCACCATTCt < 1:1662812/67‑1 (MQ=255)tAAAAGTCCATATTCGCAATCTGCTGCGTAAACTCAACGTCCGCTCACGCGTGGCGGCCACCATTCt < 1:1667741/67‑1 (MQ=255)tAAAAGTCCATATTCGCAATCTGCTGCGTAAACTCAACGTCCGCTCACGCGTGGCGGCCACCATTCt < 1:756353/67‑1 (MQ=255)tAAAAGTCCATATTCGCAATCTGCTGCGTAAACTCAACGTCCGCTCACGCGTGGCGGCCACCATTCt < 1:749868/67‑1 (MQ=255)tAAAAGTCCATATTCGCAATATGCTGCGTAAACTCAACGTCCGCTCACGCGTGGCGGCCACCATTCt < 1:1935734/67‑1 (MQ=255) aaaaGTCCATATTCGCAATCTGCTGCGTAAACTCAACGTCCGCTCACGCGTGGCGGCCACCATTCt < 1:2048600/66‑1 (MQ=255) aaaaGTCCATATTCGCAATCTGCTGCGTAAACTCAACGTCCGCTCACGCGTGGCGGCCACCATTCt < 1:1128450/66‑1 (MQ=255) aaaaGTCCATATTCGCAATCTGCTGCGTAAACTCAACGTCCGCTCACGCGTGGCGGCCACCATTCt < 1:1247346/66‑1 (MQ=255) aaaaGTCCATATTCGCAATCTGCTGCGTAAACTCAACGTCCGCTCACGCGTGGCGGCCACCATTCt < 1:1514556/66‑1 (MQ=255) aaaaGTCCATATTCGCAATCTGCTGCGTAAACTCAACGTCCGCTCACGCGTGGCGGCCACCATTCt < 1:1628708/66‑1 (MQ=255) tgcGTAAACTCAACGTCCGCTCACGCGTGGCGGCCACCATTCt < 1:1996131/43‑1 (MQ=255) tAAACTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACGATa < 1:759137/66‑1 (MQ=255) tAAACTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAATa < 1:1699704/66‑1 (MQ=255) tAAACTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAATa < 1:955521/66‑1 (MQ=255) tAAACTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAATa < 1:1130990/66‑1 (MQ=255) tAAACTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAATa < 1:1240884/66‑1 (MQ=255) tAAACTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAATa < 1:1416151/66‑1 (MQ=255) tAAACTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAATa < 1:1425948/66‑1 (MQ=255) tAAACTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAATa < 1:2203902/66‑1 (MQ=255) tAAACTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAATa < 1:1725521/66‑1 (MQ=255) tAAACTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAATa < 1:74850/66‑1 (MQ=255) tAAACTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAATa < 1:702169/66‑1 (MQ=255) tAAACTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAATa < 1:173840/66‑1 (MQ=255) tAAACTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAATa < 1:1760626/66‑1 (MQ=255) tAAACTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAATa < 1:578767/66‑1 (MQ=255) tAAACTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAATa < 1:187100/66‑1 (MQ=255) tAAACTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAATa < 1:2079479/66‑1 (MQ=255) aaaCTCAACGTCCGCTCACGCGTGGCGGCCACCATTCt < 1:805756/38‑1 (MQ=255) cTCAACGTCCGCTCACGCGTGGCGGCCACCATTCt < 1:454843/35‑1 (MQ=255) | TAAAAGTACATATTCGCAATCTGCTGCGTAAACTCAATGTCCGCTCACGCGTGGCGGCCACCATTCTGTTCCTGCAACAACGCGGGGCACAATA > minE/1418605‑1418698 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |