| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,485,630 | A→T | 100% | I70I (ATT→ATA) | menF ← | isochorismate synthase 2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,485,630 | 0 | A | T | 92.5% | 119.5 / 1.8 | 40 | I70I (ATT→ATA) | menF | isochorismate synthase 2 |
| Reads supporting (aligned to +/- strand): ref base A (0/3); new base T (0/37); total (0/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CGGGTAATCGCGCCCAGGACGACAGCTTCTTCATCACCATTACGTTGTTGCCAGTAGAATTGCGGGTA > minE/1485625‑1485692 | cGGGTTATCGCACCCAGGACGTCAGCTTCTTCATCACCATTACGTTGTTGCCAGTAGAATTGCGGGt < 1:1155116/67‑1 (MQ=255)cGGGTTATCGCACCCAGGACGGCAGCTTCTTCATCACCATTACGTTGTTGCCAGTAGAATTGCGGGt < 1:2081467/67‑1 (MQ=255)cGGGTTATCGCACCCAGGACGGCAGCTTCTTCATCACCATTACGTTGTTGCCAGTAGAATTGCGGGt < 1:965008/67‑1 (MQ=255)cGGGTTATCGCACCCAGGACGGCAGCTTCTTCATCACCATTACGTTGTTGCCAGTAGAATTGCGGGt < 1:2088394/67‑1 (MQ=255)cGGGTTATCGCACCCAGGACGGCAGCTTCTTCATCACCATTACGTTGTTGCCAGTAGAATTGCGGGt < 1:2139934/67‑1 (MQ=255)cGGGTTATCGCACCCAGGACGGCAGCTTCTTCATCACCATTACGTTGTTGCCAGTAGAATTGCGGGt < 1:2157436/67‑1 (MQ=255)cGGGTTATCGCACCCAGGACGGCAGCTTCTTCATCACCATTACGTTGTTGCCAGTAGAATTGCGGGt < 1:2277956/67‑1 (MQ=255)cGGGTTATCGCACCCAGGACGGCAGCTTCTTCATCACCATTACGTTGTTGCCAGTAGAATTGCGGGt < 1:248765/67‑1 (MQ=255)cGGGTTATCGCACCCAGGACGGCAGCTTCTTCATCACCATTACGTTGTTGCCAGTAGAATTGCGGGt < 1:342062/67‑1 (MQ=255)cGGGTTATCGCACCCAGGACGGCAGCTTCTTCATCACCATTACGTTGTTGCCAGTAGAATTGCGGGt < 1:392262/67‑1 (MQ=255)cGGGTTATCGCACCCAGGACGGCAGCTTCTTCATCACCATTACGTTGTTGCCAGTAGAATTGCGGGt < 1:485011/67‑1 (MQ=255)cGGGTTATCGCACCCAGGACGGCAGCTTCTTCATCACCATTACGTTGTTGCCAGTAGAATTGCGGGt < 1:548629/67‑1 (MQ=255)cGGGTTATCGCACCCAGGACGGCAGCTTCTTCATCACCATTACGTTGTTGCCAGTAGAATTGCGGGt < 1:611720/67‑1 (MQ=255)cGGGTTATCGCACCCAGGACGGCAGCTTCTTCATCACCATTACGTTGTTGCCAGTAGAATTGCGGGt < 1:61487/67‑1 (MQ=255)cGGGTTATCGCACCCAGGACGGCAGCTTCTTCATCACCATTACGTTGTTGCCAGTAGAATTGCGGGt < 1:692303/67‑1 (MQ=255)cGGGTTATCGCACCCAGGACGGCAGCTTCTTCATCACCATTACGTTGTTGCCAGTAGAATTGCGGGt < 1:745211/67‑1 (MQ=255)cGGGTTATCGCACCCAGGACGGCAGCTTCTTCATCACCATTACGTTGTTGCCAGTAGAATTGCGGGt < 1:754271/67‑1 (MQ=255)cGGGTTATCGCACCCAGGACGGCAGCTTCTTCATCACCATTACGTTGTTGCCAGTAGAATTGCGGGt < 1:881671/67‑1 (MQ=255)cGGGTTATCGCACCCAGGACGGCAGCTTCTTCATCACCATTACGTTGTTGCCAGTAGAATTGCGGGt < 1:882197/67‑1 (MQ=255)cGGGTTATCGCACCCAGGACGGCAGCTTCTTCATCACCATTACGTTGTTGCCAGTAGAATTGCGGGt < 1:1029931/67‑1 (MQ=255)cGGGTTATCGCACCCAGGACGGCAGCTTCTTCATCACCATTACGTTGTTGCCAGTAGAATTGCGGGt < 1:1943513/67‑1 (MQ=255)cGGGTTATCGCACCCAGGACGGCAGCTTCTTCATCACCATTACGTTGTTGCCAGTAGAATTGCGGGt < 1:1183968/67‑1 (MQ=255)cGGGTTATCGCACCCAGGACGGCAGCTTCTTCATCACCATTACGTTGTTGCCAGTAGAATTGCGGGt < 1:1230626/67‑1 (MQ=255)cGGGTTATCGCACCCAGGACGGCAGCTTCTTCATCACCATTACGTTGTTGCCAGTAGAATTGCGGGt < 1:127899/67‑1 (MQ=255)cGGGTTATCGCACCCAGGACGGCAGCTTCTTCATCACCATTACGTTGTTGCCAGTAGAATTGCGGGt < 1:130347/67‑1 (MQ=255)cGGGTTATCGCACCCAGGACGGCAGCTTCTTCATCACCATTACGTTGTTGCCAGTAGAATTGCGGGt < 1:1330974/67‑1 (MQ=255)cGGGTTATCGCACCCAGGACGGCAGCTTCTTCATCACCATTACGTTGTTGCCAGTAGAATTGCGGGt < 1:1337466/67‑1 (MQ=255)cGGGTTATCGCACCCAGGACGGCAGCTTCTTCATCACCATTACGTTGTTGCCAGTAGAATTGCGGGt < 1:1432514/67‑1 (MQ=255)cGGGTTATCGCACCCAGGACGGCAGCTTCTTCATCACCATTACGTTGTTGCCAGTAGAATTGCGGGt < 1:145239/67‑1 (MQ=255)cGGGTTATCGCACCCAGGACGGCAGCTTCTTCATCACCATTACGTTGTTGCCAGTAGAATTGCGGGt < 1:1537427/67‑1 (MQ=255)cGGGTTATCGCACCCAGGACGGCAGCTTCTTCATCACCATTACGTTGTTGCCAGTAGAATTGCGGGt < 1:1559485/67‑1 (MQ=255)cGGGTTATCGCACCCAGGACGGCAGCTTCTTCATCACCATTACGTTGTTGCCAGTAGAATTGCGGGt < 1:1574674/67‑1 (MQ=255)cGGGTTATCGCACCCAGGACGGCAGCTTCTTCATCACCATTACGTTGTTGCCAGTAGAATTGCGGGt < 1:1575638/67‑1 (MQ=255)cGGGTTATCGCACCCAGGACGGCAGCTTCTTCATCACCATTACGTTGTTGCCAGTAGAATTGCGGGt < 1:1588538/67‑1 (MQ=255)cGGGTTATCGCACCCAGGACGGCAGCTTCTTCATCACCATTACGTTGTTGCCAGTAGAATTGCGGGt < 1:1735032/67‑1 (MQ=255)cGGGTTATCGCACCCAGGACGGCAGCTTCTTCATCACCATTACGTTGTTGCCAGTAGAATTGCGGGt < 1:1916310/67‑1 (MQ=255)cGGGTTATCGCACCCAGGACGGCAGCTTCTTCATCACCATTACGTTGTTGCCAGTAGAATTGCGGGt < 1:2038173/67‑1 (MQ=255) gggTAATCGCGCCCAGGACGACAGCTTCTTCATCACCATTACGTTGTTGCCAGTAGAATTGCGGGTa < 1:509115/67‑1 (MQ=255) gggTAATCGCGCCCAGGACGACAGCTTCTTCATCACCATTACGTTGTTGCCAGTAGAATTGCGGGTa < 1:252444/67‑1 (MQ=255) gggTAATCGCGCCCAGGACGACAGCTTCTTCATCACCATTACGTTGTTGCCAGTAGAATTGCGGGTa < 1:157717/67‑1 (MQ=255) | CGGGTAATCGCGCCCAGGACGACAGCTTCTTCATCACCATTACGTTGTTGCCAGTAGAATTGCGGGTA > minE/1485625‑1485692 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |