Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	204,511	C→T	100%	intergenic (+111/‑73)	alaV → / → rrlH	tRNA‑Ala/23S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	204,511	0	C	T	100.0%	117.4 / NA	35	intergenic (+111/‑73)	alaV/rrlH	tRNA‑Ala/23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (33/2);  total (33/2)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ACACTGAACAACGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGCAAGAAACATC  >  minE/204500‑204571            |                                                             acacTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGaa       <  1:1359627/67‑1 (MQ=255)     tGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATATTCGCAACACGATGATGGATCGCAAGaa       <  1:1484773/63‑1 (MQ=11)      gAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACAt   >  1:207121/1‑66 (MQ=255)      gAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACAt   >  1:1935983/1‑66 (MQ=255)      gAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACAt   >  1:1076716/1‑66 (MQ=255)      gAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACAt   >  1:1816803/1‑66 (MQ=255)      gAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACAt   >  1:1667978/1‑66 (MQ=255)      gAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACAt   >  1:400183/1‑66 (MQ=255)      gAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACAt   >  1:543983/1‑66 (MQ=255)      gAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATc  >  1:529461/1‑67 (MQ=255)      gAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATc  >  1:551954/1‑67 (MQ=255)      gAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATc  >  1:436206/1‑67 (MQ=255)      gAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATc  >  1:601743/1‑67 (MQ=255)      gAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATc  >  1:347864/1‑67 (MQ=255)      gAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATc  >  1:77147/1‑67 (MQ=255)      gAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATc  >  1:336656/1‑67 (MQ=255)      gAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATc  >  1:787595/1‑67 (MQ=255)      gAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATc  >  1:945392/1‑67 (MQ=255)      gAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATc  >  1:953884/1‑67 (MQ=255)      gAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATc  >  1:2139118/1‑67 (MQ=255)      gAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATc  >  1:1918910/1‑67 (MQ=255)      gAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATc  >  1:1997196/1‑67 (MQ=255)      gAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATc  >  1:1951826/1‑67 (MQ=255)      gAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATc  >  1:1812068/1‑67 (MQ=255)      gAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATc  >  1:16497/1‑67 (MQ=255)      gAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATc  >  1:1558878/1‑67 (MQ=255)      gAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATc  >  1:1509029/1‑67 (MQ=255)      gAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATc  >  1:1408433/1‑67 (MQ=255)      gAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATc  >  1:1327524/1‑67 (MQ=255)      gAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATc  >  1:1299121/1‑67 (MQ=255)      gAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATc  >  1:1258830/1‑67 (MQ=255)      gAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATc  >  1:1228465/1‑67 (MQ=255)      gAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATc  >  1:1222526/1‑67 (MQ=255)      gAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATc  >  1:1174072/1‑67 (MQ=255)      gAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATc  >  1:1078723/1‑67 (MQ=255)            |                                                             ACACTGAACAACGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGCAAGAAACATC  >  minE/204500‑204571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑