| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,504,431 | C→A | 100% | S295R (AGC→AGA) | yfbQ → | predicted aminotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,504,431 | 0 | C | A | 100.0% | 14.3 / NA | 6 | S295R (AGC→AGA) | yfbQ | predicted aminotransferase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (6/0); total (6/0) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CGCTAGGTGGTTATCAGAGCATCAGTGAATTTATTACCCCTGGCGGTCGTCTTTATGAGCAGCGTAA > minE/1504412‑1504478 | cgcTAGGTGGTTATCAGAGAATCAGTGAATTTATTAcccc > 1:1142608/1‑40 (MQ=255)cgcTAGGTGGTTATCAGAGAATCAGTGAATTTATTACCCCTGGCGGTCGTCTTTATGAGCAGCGTaa > 1:1835250/1‑67 (MQ=255)cgcTAGGTGGTTATCAGAGAATCAGTGAATTTATTACCCCTGGCGGTCGTCTTTATGAGCAGCGTaa > 1:393958/1‑67 (MQ=255)cgcTAGGTGGTTATCAGAGAATCAGTGAATTTATTACCCCTGGCGGTCGTCTTTATGAGCAGCGTaa > 1:913765/1‑67 (MQ=255)cgcTAGGTGGTTATCAGAGAATCAGTGAATTTATTACCCCTGGCGGTCGTCTTTATGAGCAGCGTa > 1:830035/1‑66 (MQ=255)cgcTAGGTGGTTATCAGAGAATCAGTGAATTTATTACCACTGGCGGTCGTCTTTATGAGCAGCGTaa > 1:1067451/1‑67 (MQ=255) | CGCTAGGTGGTTATCAGAGCATCAGTGAATTTATTACCCCTGGCGGTCGTCTTTATGAGCAGCGTAA > minE/1504412‑1504478 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |