| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,511,807 | G→A | 100% | E359K (GAG→AAG) | pta → | phosphate acetyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,511,807 | 0 | G | A | 91.7% | 29.0 / ‑3.5 | 12 | E359K (GAG→AAG) | pta | phosphate acetyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (11/0); total (12/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.73e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCT > minE/1511756‑1511830 | agacTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCAAGAAAGTTCAGGaa > 1:2200741/4‑66 (MQ=255)aGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCAAGATAGTTCAGGAAt > 1:635925/1‑67 (MQ=255)aGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCAAGAAAGTTCAGGAAt > 1:1061332/1‑67 (MQ=255)aGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCAAGAAAGTTCAGGAAt > 1:1480404/1‑67 (MQ=255)aGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCAAGAAAGTTCAGGAAt > 1:482571/1‑67 (MQ=255)aGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCAAGAAAGTTCAGGAAt > 1:507474/1‑67 (MQ=255)aGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCAAGAAAGTTCAGGAAt > 1:580873/1‑67 (MQ=255)aGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCAAGAAAGTTCAGGAAt > 1:615336/1‑67 (MQ=255)aGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCAAGAAAGTTCAGGAAt > 1:801271/1‑67 (MQ=255)aGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCAAGAAAGTTCAGGAAt > 1:819905/1‑67 (MQ=255)aGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCAAGAAAGTTCAGGAAt > 1:824771/1‑67 (MQ=255) aCGAACGTATCGAGAAAGTTCAGGAATACGTTGCt > 1:1941821/1‑35 (MQ=255) | AGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCT > minE/1511756‑1511830 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |