| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,541,194 | A→T | 100% | intergenic (‑296/+385) | yfcV ← / ← sixA | predicted fimbrial‑like adhesin protein/phosphohistidine phosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,541,194 | 0 | A | T | 76.5% | 28.7 / 6.4 | 17 | intergenic (‑296/+385) | yfcV/sixA | predicted fimbrial‑like adhesin protein/phosphohistidine phosphatase |
| Reads supporting (aligned to +/- strand): ref base A (0/4); new base T (13/0); total (13/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.20e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ATCTTAAGGATCTTCTAAAAAAACACGAAATATATATTTAGAAAAAACAGGAAGCATATCAACAATGAATGCCAATAGCTTAAAAAACAAACAATGAACATATAATGCGATTGGCATTAACCC > minE/1541134‑1541256 | aTCTTAAGGATCTTCTAAAAAAACACGTAATATATATTTAGAAAAAACAGGAAGCATATCAACaatg < 1:1716808/67‑1 (MQ=255)aTCTTAAGGATCTTCTAAAAAAACACGAAATATATATTTAGAAAAAACAGGAAGCATATCAACaatg < 1:1019397/67‑1 (MQ=255)aTCTTAAGGATCTTCTAAAAAAACACGAAATATATATTTAGAAAAAACAGGAAGCATATCAACaatg < 1:1191826/67‑1 (MQ=255)aTCTTAAGGATCTTCTAAAAAAACACGAAATATATATTTAGAAAAAACAGGAAGCATATCAACaatg < 1:1457899/67‑1 (MQ=255) gatCTACAATGAATGCCAATAGCTTAAAAAACAAACAATGa > 1:1544901/2‑41 (MQ=255) gatCTACAATGAATGCCAATAGCTTAAAAAACAAACAATGAACa > 1:13089/2‑44 (MQ=255) gatCTACAATGAATGCCAATAGCTTAAAAAACAAACAATGAACATATAATGCGATTGGCATTAAccc > 1:916221/2‑67 (MQ=255) gatCTACAATGAATGCCAATAGCTTAAAAAACAAACAATGAACATATAATGCGATTGGCATTAAccc > 1:787634/2‑67 (MQ=255) gatCTACAATGAATGCCAATAGCTTAAAAAACAAACAATGAACATATAATGCGATTGGCATTAAccc > 1:55329/2‑67 (MQ=255) gatCTACAATGAATGCCAATAGCTTAAAAAACAAACAATGAACATATAATGCGATTGGCATTAAccc > 1:2139256/2‑67 (MQ=255) gatCTACAATGAATGCCAATAGCTTAAAAAACAAACAATGAACATATAATGCGATTGGCATTAAccc > 1:1665731/2‑67 (MQ=255) gatCTACAATGAATGCCAATAGCTTAAAAAACAAACAATGAACATATAATGCGATTGGCATTAAccc > 1:160601/2‑67 (MQ=255) gatCTACAATGAATGCCAATAGCTTAAAAAACAAACAATGAACATATAATGCGATTGGCATTAAccc > 1:1547484/2‑67 (MQ=255) gatCTACAATGAATGCCAATAGCTTAAAAAACAAACAATGAACATATAATGCGATTGGCATTAAccc > 1:144185/2‑67 (MQ=255) gatCTACAATGAATGCCAATAGCTTAAAAAACAAACAATGAACATATAATGCGATTGGCATTAAccc > 1:1255290/2‑67 (MQ=255) gatCTACAATGAATGCCAATAGCTTAAAAAACAAACAATGAACATATAATGCGATTGGCATTAAccc > 1:1132662/2‑67 (MQ=255) gatCTACAATGAATGCCAATAGCTTAAAAAACAAACAATGAACATATAATGCGATTGGCATTAAccc > 1:1040637/2‑67 (MQ=255) | ATCTTAAGGATCTTCTAAAAAAACACGAAATATATATTTAGAAAAAACAGGAAGCATATCAACAATGAATGCCAATAGCTTAAAAAACAAACAATGAACATATAATGCGATTGGCATTAACCC > minE/1541134‑1541256 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |