| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,590,218 | A→C | 100% | V26V (GTT→GTG) | pdxK ← | pyridoxal‑pyridoxamine kinase/hydroxymethylpyrimidine kinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,590,218 | 0 | A | C | 87.0% | 235.6 / 16.6 | 77 | V26V (GTT→GTG) | pdxK | pyridoxal‑pyridoxamine kinase/hydroxymethylpyrimidine kinase |
| Reads supporting (aligned to +/- strand): ref base A (10/0); new base C (0/67); total (10/67) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.12e-13 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.08e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGCCCTACT > minE/1590182‑1590265 | aGCGGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACGGCAACGATATcc < 1:2211755/67‑1 (MQ=255)aGCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACGGCAACGATATcc < 1:217099/67‑1 (MQ=255)aGCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACGGCAACGATATcc < 1:314216/67‑1 (MQ=255)aGCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACGGCAACGATATcc < 1:308042/67‑1 (MQ=255)aGCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACGGCAACGATATcc < 1:296833/67‑1 (MQ=255)aGCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACGGCAACGATATcc < 1:225517/67‑1 (MQ=255)aGCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACGGCAACGATATcc < 1:2210821/67‑1 (MQ=255)aGCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACGGCAACGATATcc < 1:2187410/67‑1 (MQ=255)aGCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACGGCAACGATATcc < 1:2178575/67‑1 (MQ=255)aGCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACGGCAACGATATcc < 1:333990/67‑1 (MQ=255)aGCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACGGCAACGATATcc < 1:2160310/67‑1 (MQ=255)aGCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACGGCAACGATATcc < 1:2080114/67‑1 (MQ=255)aGCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACGGCAACGATATcc < 1:2042338/67‑1 (MQ=255)aGCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACGGCAACGATATcc < 1:2042272/67‑1 (MQ=255)aGCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACGGCAACGATATcc < 1:2041878/67‑1 (MQ=255)aGCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACGGCAACGATATcc < 1:1995600/67‑1 (MQ=255)aGCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACGGCAACGATATcc < 1:1985239/67‑1 (MQ=255)aGCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACGGCAACGATATcc < 1:1046851/67‑1 (MQ=255)aGCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACGGCAACGATATcc < 1:361056/67‑1 (MQ=255)aGCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACGGCAACGATATcc < 1:40653/67‑1 (MQ=255)aGCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACGGCAACGATATcc < 1:435643/67‑1 (MQ=255)aGCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACGGCAACGATATcc < 1:47983/67‑1 (MQ=255)aGCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACGGCAACGATATcc < 1:504729/67‑1 (MQ=255)aGCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACGGCAACGATATcc < 1:581657/67‑1 (MQ=255)aGCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACGGCAACGATATcc < 1:612362/67‑1 (MQ=255)aGCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACGGCAACGATATcc < 1:620822/67‑1 (MQ=255)aGCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACGGCAACGATATcc < 1:624866/67‑1 (MQ=255)aGCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACGGCAACGATATcc < 1:73864/67‑1 (MQ=255)aGCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACGGCAACGATATcc < 1:743756/67‑1 (MQ=255)aGCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACGGCAACGATATcc < 1:762893/67‑1 (MQ=255)aGCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACGGCAACGATATcc < 1:835520/67‑1 (MQ=255)aGCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACGGCAACGATATcc < 1:928313/67‑1 (MQ=255)aGCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACGGCAACGATATcc < 1:985483/67‑1 (MQ=255)aGCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACGGCAACGATATcc < 1:1797740/67‑1 (MQ=255)aGCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACGGCAACGATATcc < 1:1606390/67‑1 (MQ=255)aGCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACGGCAACGATATcc < 1:166879/67‑1 (MQ=255)aGCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACGGCAACGATATcc < 1:1685572/67‑1 (MQ=255)aGCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACGGCAACGATATcc < 1:1556276/67‑1 (MQ=255)aGCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACGGCAACGATATcc < 1:1716855/67‑1 (MQ=255)aGCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACGGCAACGATATcc < 1:1736045/67‑1 (MQ=255)aGCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACGGCAACGATATcc < 1:1744474/67‑1 (MQ=255)aGCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACGGCAACGATATcc < 1:148463/67‑1 (MQ=255)aGCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACGGCAACGATATcc < 1:1440229/67‑1 (MQ=255)aGCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACGGCAACGATATcc < 1:1795392/67‑1 (MQ=255)aGCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACGGCAACGATATcc < 1:1818790/67‑1 (MQ=255)aGCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACGGCAACGATATcc < 1:1315904/67‑1 (MQ=255)aGCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACGGCAACGATATcc < 1:1281460/67‑1 (MQ=255)aGCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACGGCAACGATATcc < 1:1228568/67‑1 (MQ=255)aGCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACGGCAACGATATcc < 1:1126662/67‑1 (MQ=255)aGCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACGGCAACGATATcc < 1:1102636/67‑1 (MQ=255)aGCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACGGCAACGATATcc < 1:1080144/67‑1 (MQ=255)aGCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACGGCAACGATATcc < 1:1061568/67‑1 (MQ=255)aGCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACGGCAACGATATcc < 1:1034366/67‑1 (MQ=255)aGCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACGGCAACGATATcc < 1:1977022/67‑1 (MQ=255) gCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTTCACTACCTGCGACTGCACGGCAACGATATcc < 1:1960028/66‑1 (MQ=255) gCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACGGCAACGATATcc < 1:1640522/66‑1 (MQ=255) gCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACGGCAACGATATcc < 1:293570/66‑1 (MQ=255) gCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACGGCAACGATATcc < 1:316397/66‑1 (MQ=255) gCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACGGCAACGATATcc < 1:2190066/66‑1 (MQ=255) gCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACGGCAACGATATcc < 1:1902348/66‑1 (MQ=255) gCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACGGCAACGATATcc < 1:520302/66‑1 (MQ=255) gCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACGGCAACGATATcc < 1:2140351/66‑1 (MQ=255) gCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACGGCAACGATATcc < 1:1157490/66‑1 (MQ=255) gCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACGGCAACGATATcc < 1:132576/62‑1 (MQ=255) gTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCTCCTGCAGTGCCCTAct > 1:175907/1‑66 (MQ=255) gTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGCCCTAct > 1:1922995/1‑66 (MQ=255) gTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGCCCTAct > 1:968662/1‑66 (MQ=255) gTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGCCCTAct > 1:911470/1‑66 (MQ=255) gTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGCCCTAct > 1:748715/1‑66 (MQ=255) gTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGCCCTAct > 1:1822590/1‑66 (MQ=255) gTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGCCCTAct > 1:512356/1‑66 (MQ=255) gTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGCCCTAct > 1:1484194/1‑66 (MQ=255) gTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGCCCTAct > 1:1536104/1‑66 (MQ=255) gTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGAGCactact > 1:213137/1‑66 (MQ=255) gCCTACGCTGCCGTACACTACCTGCGACTGCACGGCAACGATATcc < 1:2125895/46‑1 (MQ=255) gCCTACGCTGCCGTACACTACCTGCGACTGCACGGCAACGATATcc < 1:2054985/46‑1 (MQ=255) ctaCGCTGCCGTACACTACCTGCGACTGCACGGCAACGATATcc < 1:1874795/42‑1 (MQ=255) | AGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGCCCTACT > minE/1590182‑1590265 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |