| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,622,452 | G→A | 100% | H732Y (CAC→TAC) | maeB ← | fused malic enzyme predicted oxidoreductase and predicted phosphotransacetylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,622,452 | 0 | G | A | 93.4% | 40.4 / ‑3.6 | 15 | H732Y (CAC→TAC) | maeB | fused malic enzyme predicted oxidoreductase and predicted phosphotransacetylase |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (0/14); total (1/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.67e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AGCGCCACCATGTTGACGATACGACGCACCGATGCGATCGGCGTTAACACGTGAACCGGTTTCGCCACACCCATCAGCACCGGGCCGACAGTCACACCTTCCGAGCTGGAAACA > minE/1622400‑1622513 | aGCGCCACCATGTTGACGATACGACGCACCGATGCGATCGGCGTTAACACGTAAACCGGTTTCGCca < 1:1070052/67‑1 (MQ=255)aGCGCCACCATGTTGACGATACGACGCACCGATGCGATCGGCGTTAACACGTAAACCGGTTTCGCca < 1:1119415/67‑1 (MQ=255)aGCGCCACCATGTTGACGATACGACGCACCGATGCGATCGGCGTTAACACGTAAACCGGTTTCGCca < 1:1617650/67‑1 (MQ=255)aGCGCCACCATGTTGACGATACGACGCACCGATGCGATCGGCGTTAACACGTAAACCGGTTTCGCca < 1:1781374/67‑1 (MQ=255)aGCGCCACCATGTTGACGATACGACGCACCGATGCGATCGGCGTTAACACGTAAACCGGTTTCGCca < 1:1783429/67‑1 (MQ=255)aGCGCCACCATGTTGACGATACGACGCACCGATGCGATCGGCGTTAACACGTAAACCGGTTTCGCca < 1:1865383/67‑1 (MQ=255)aGCGCCACCATGTTGACGATACGACGCACCGATGCGATCGGCGTTAACACGTAAACCGGTTTCGCca < 1:2032836/67‑1 (MQ=255)aGCGCCACCATGTTGACGATACGACGCACCGATGCGATCGGCGTTAACACGTAAACCGGTTTCGCca < 1:2313371/67‑1 (MQ=255)aGCGCCACCATGTTGACGATACGACGCACCGATGCGATCGGCGTTAACACGTAAACCGGTTTCGCca < 1:67388/67‑1 (MQ=255)aGCGCCACCATGTTGACGATACGACGCACCGATGCGATCGGCGTTAACACGTAAACCGGTTTCGCca < 1:705005/67‑1 (MQ=255)aGCGCCACCATGTTGACGATACGACGCACCGATGCGATCGGCGTTAACACGTAAACCGGTTTCGCca < 1:902518/67‑1 (MQ=255) gcgcCACCATGTTGACGATACGACGCACCGATGCGATCGGCGTTAACACGTAAACCGGTTTCGCca < 1:1038418/66‑1 (MQ=255) gcgcCACCATGTTGACGATACGACGCACCGATGCGATCGGCGTTAACACGTAAACCGGTTTCGCca < 1:1974760/66‑1 (MQ=255) gcgcCACCATGTTGACGATACGACGCACCGATGCGATCGGCGTTAACACGTAAACCGGTTTCGCca < 1:280744/66‑1 (MQ=255) cacGTGAACCGGTTTCGCCACACCCATCAGCACCGGGCCGACAGTCACACCTTCCGAGCTGGAAaca > 1:1621352/1‑67 (MQ=255) | AGCGCCACCATGTTGACGATACGACGCACCGATGCGATCGGCGTTAACACGTGAACCGGTTTCGCCACACCCATCAGCACCGGGCCGACAGTCACACCTTCCGAGCTGGAAACA > minE/1622400‑1622513 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |