| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,697,000 | T→C | 100% | intergenic (+69/‑49) | suhB → / → yfhR | inositol monophosphatase/predicted peptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,697,000 | 0 | T | C | 85.7% | 46.2 / 1.7 | 21 | intergenic (+69/‑49) | suhB/yfhR | inositol monophosphatase/predicted peptidase |
| Reads supporting (aligned to +/- strand): ref base T (3/0); new base C (18/0); total (21/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.72e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TATCACTCACCCGCCCTCGCCTTTCAGGCGCTATTCCGAAATACTTCCTCACTGCTTTACTTTCTTT > minE/1696948‑1697014 | tatCACTCACCCGCCCTCGCCTTTCAGGCGCTATTCCGAAATACTTCCTCACTGCTTTActttcttt > 1:2315388/1‑67 (MQ=255)tatCACTCACCCGCCCTCGCCTTTCAGGCGCTATTCCGAAATACTTCCTCACTGCTTTActttcttt > 1:1137045/1‑67 (MQ=255)tatCACTCACCCGCCCTCGCCTTTCAGGCGCTATTCCGAAATACTTCCTCACTGCTTTActttcttt > 1:2082047/1‑67 (MQ=255) cTTTCAGGCGCTATTCCGAAATACTTCCTCACCGCttt > 1:2159895/1‑38 (MQ=255) cTTTCAGGCGCTATTCCGAAATACTTCCTCACCGCTTTa > 1:2053752/1‑39 (MQ=255) cTTTCAGGCGCTATTCCGAAATACTTCCTCACCGCTTTa > 1:707820/1‑39 (MQ=255) cTTTCAGGCGCTATTCCGAAATACTTCCTCACCGCTTTa > 1:671273/1‑39 (MQ=255) cTTTCAGGCGCTATTCCGAAATACTTCCTCACCGCTTTa > 1:452919/1‑39 (MQ=255) cTTTCAGGCGCTATTCCGAAATACTTCCTCACCGCTTTa > 1:418604/1‑39 (MQ=255) cTTTCAGGCGCTATTCCGAAATACTTCCTCACCGCTTTa > 1:396285/1‑39 (MQ=255) cTTTCAGGCGCTATTCCGAAATACTTCCTCACCGCTTTa > 1:27633/1‑39 (MQ=255) cTTTCAGGCGCTATTCCGAAATACTTCCTCACCGCTTTa > 1:2314439/1‑39 (MQ=255) cTTTCAGGCGCTATTCCGAAATACTTCCTCACCGCTTTa > 1:1954234/1‑39 (MQ=255) cTTTCAGGCGCTATTCCGAAATACTTCCTCACCGCTTTa > 1:1855693/1‑39 (MQ=255) cTTTCAGGCGCTATTCCGAAATACTTCCTCACCGCTTTa > 1:1814932/1‑39 (MQ=255) cTTTCAGGCGCTATTCCGAAATACTTCCTCACCGCTTTa > 1:1807330/1‑39 (MQ=255) cTTTCAGGCGCTATTCCGAAATACTTCCTCACCGCTTTa > 1:1692772/1‑39 (MQ=255) cTTTCAGGCGCTATTCCGAAATACTTCCTCACCGCTTTa > 1:1663620/1‑39 (MQ=255) cTTTCAGGCGCTATTCCGAAATACTTCCTCACCGCTTTa > 1:1512074/1‑39 (MQ=255) cTTTCAGGCGCTATTCCGAAATACTTCCTCACCGCTTTa > 1:1191097/1‑39 (MQ=255) cTTTCAGGCGCTATTCCGAAATACTTCCTCACCGCTTTa > 1:1190934/1‑39 (MQ=255) | TATCACTCACCCGCCCTCGCCTTTCAGGCGCTATTCCGAAATACTTCCTCACTGCTTTACTTTCTTT > minE/1696948‑1697014 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |