| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,699,474 | C→G | 100% | L353L (CTG→CTC) | hcaT ← | predicted 3‑phenylpropionic transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,699,474 | 0 | C | G | 75.4% | 258.1 / 90.5 | 134 | L353L (CTG→CTC) | hcaT | predicted 3‑phenylpropionic transporter |
| Reads supporting (aligned to +/- strand): ref base C (2/31); new base G (101/0); total (103/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.16e-28 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
Only 100 of 134 total aligned reads displayed.
GGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCAT > minE/1699416‑1699530 | ggCGCAAAAACATTGCCGGAAGCTCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATAtt < 1:2017930/66‑1 (MQ=255)ggCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATAtt < 1:1071169/66‑1 (MQ=255)ggCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATAtt < 1:436275/66‑1 (MQ=255)ggCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATAtt < 1:1867648/66‑1 (MQ=255)ggCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATAtt < 1:1838521/66‑1 (MQ=255)ggCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATAtt < 1:468517/66‑1 (MQ=255)ggCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATAtt < 1:48353/66‑1 (MQ=255)ggCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATAtt < 1:1893904/66‑1 (MQ=255)ggCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATAtt < 1:1674946/66‑1 (MQ=255)ggCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATAtt < 1:357868/66‑1 (MQ=255)ggCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATAtt < 1:1639371/66‑1 (MQ=255)ggCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATAtt < 1:1552667/66‑1 (MQ=255)ggCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATAtt < 1:1516801/66‑1 (MQ=255)ggCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATAtt < 1:2138424/66‑1 (MQ=255)ggCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATAtt < 1:878201/66‑1 (MQ=255)ggCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATAtt < 1:1077722/66‑1 (MQ=255)ggCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATAtt < 1:2216021/66‑1 (MQ=255)ggCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATAtt < 1:2167479/66‑1 (MQ=255)ggCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATAtt < 1:1232689/66‑1 (MQ=255)ggCGCAAAAACATTGCAGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATAtt < 1:886410/66‑1 (MQ=255) ccATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGc < 1:621189/67‑1 (MQ=255) cATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGc < 1:1474992/66‑1 (MQ=255) cATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGc < 1:198343/66‑1 (MQ=255) cAGACCACGCCGTGGCCGAGATATTGATACAGGCAACCGGGGAAAACGGTCATGATGGCGATACTgc > 1:829266/1‑67 (MQ=255) cAGAACACGCCGTGGCGGAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATGGCGATACTgc > 1:2089646/1‑67 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGaa > 1:912207/1‑35 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAACGGTGATGATGGCGATa > 1:1127478/1‑63 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAACGGTCATg > 1:1442900/1‑54 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATGGCGATACt > 1:279948/1‑65 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATGGCGATACt > 1:896375/1‑65 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATGGCGATACTgc > 1:2229203/1‑67 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATGGCGATACTgc > 1:992848/1‑67 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATGGCGATACTgc > 1:2241961/1‑67 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATGGCGATACTgc > 1:347064/1‑67 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATGGCGATACTgc > 1:297302/1‑67 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATGGCGATACTgc > 1:2314988/1‑67 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATGGCGATACTgc > 1:2308218/1‑67 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATGGCGATACTgc > 1:2282733/1‑67 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATGGCGATACTgc > 1:227772/1‑67 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATGGCGATACTgc > 1:2247508/1‑67 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATGGCGATACTgc > 1:686719/1‑67 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATGGCGATACTgc > 1:1041046/1‑67 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATGGCGATACTgc > 1:839151/1‑67 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATGGCGATACTgc > 1:78832/1‑67 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATGGCGATACTgc > 1:786718/1‑67 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATGGCGATACTgc > 1:784085/1‑67 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATGGCGATACTgc > 1:728477/1‑67 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATGGCGATACTgc > 1:718100/1‑67 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATGGCGATACTgc > 1:690468/1‑67 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATGGCGATACTgc > 1:687803/1‑67 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATGGCGATACTgc > 1:452642/1‑67 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATGGCGATACTgc > 1:681549/1‑67 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATGGCGATACTgc > 1:641950/1‑67 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATGGCGATACTgc > 1:625013/1‑67 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATGGCGATACTgc > 1:613132/1‑67 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATGGCGATACTgc > 1:58557/1‑67 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATGGCGATACTgc > 1:573680/1‑67 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATGGCGATACTgc > 1:53171/1‑67 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATGGCGATACTgc > 1:517823/1‑67 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATGGCGATACTgc > 1:1418935/1‑67 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATGGCGATACTgc > 1:2227499/1‑67 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATGGCGATACTgc > 1:1612816/1‑67 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATGGCGATACTgc > 1:1566473/1‑67 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATGGCGATACTgc > 1:1539633/1‑67 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATGGCGATACTgc > 1:1538460/1‑67 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATGGCGATACTgc > 1:1505041/1‑67 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATGGCGATACTgc > 1:1475074/1‑67 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATGGCGATACTgc > 1:1440107/1‑67 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATGGCGATACTgc > 1:143733/1‑67 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATGGCGATACTgc > 1:1614461/1‑67 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATGGCGATACTgc > 1:1413903/1‑67 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATGGCGATACTgc > 1:1405119/1‑67 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATGGCGATACTgc > 1:1343571/1‑67 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATGGCGATACTgc > 1:1226894/1‑67 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATGGCGATACTgc > 1:1186531/1‑67 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATGGCGATACTgc > 1:1179967/1‑67 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATGGCGATACTgc > 1:1176089/1‑67 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATGGCGATACTgc > 1:111070/1‑67 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATGGCGATACTgc > 1:1094077/1‑67 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATGGCGATACTgc > 1:1923470/1‑67 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATGGCGATACTgc > 1:2155741/1‑67 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATGGCGATACTgc > 1:2123596/1‑67 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATGGCGATACTgc > 1:2084151/1‑67 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATGGCGATACTgc > 1:2063568/1‑67 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATGGCGATACTgc > 1:200226/1‑67 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATGGCGATACTgc > 1:1984632/1‑67 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATGGCGATACTgc > 1:1983351/1‑67 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATGGCGATACTgc > 1:1971491/1‑67 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATGGCGATACTgc > 1:1968421/1‑67 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATGGCGATACTgc > 1:1623766/1‑67 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATGGCGATACTgc > 1:1839374/1‑67 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATGGCGATACTgc > 1:1821247/1‑67 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATGGCGATACTgc > 1:1729937/1‑67 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATGGCGATACTgc > 1:1705127/1‑67 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATGGCGATACTgc > 1:1653975/1‑67 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATGGCGATACTgc > 1:1645453/1‑67 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATGGCGATACTg > 1:989791/1‑66 (MQ=255) cAGAACACGCCGTGGCCGAGATATTGATACAGGAAACCGGCGAAAAACGTCATGATGGCGATACTgc > 1:1580771/1‑67 (MQ=255) cGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCAt > 1:1861585/1‑67 (MQ=255) cGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCAt > 1:505227/1‑67 (MQ=255) | GGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCAT > minE/1699416‑1699530 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |