| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,719,969 | A→G | 100% | H42H (CAT→CAC) | glnB ← | regulatory protein P‑II for glutamine synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,719,969 | 0 | A | G | 100.0% | 26.1 / NA | 9 | H42H (CAT→CAC) | glnB | regulatory protein P‑II for glutamine synthetase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (0/9); total (0/9) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCAT > minE/1719961‑1720013 | aGCTCGGTGTGACCCTTCTGGCGACCGAAACCCTTCACTTCGGTCACCGTCAt < 1:1013692/53‑1 (MQ=255)aGCTCGGTGTGACCCTTCTGGCGACCGAAACCCTTCACTTCGGTCACCGTCAt < 1:10463/53‑1 (MQ=255)aGCTCGGTGTGACCCTTCTGGCGACCGAAACCCTTCACTTCGGTCACCGTCAt < 1:1277487/53‑1 (MQ=255)aGCTCGGTGTGACCCTTCTGGCGACCGAAACCCTTCACTTCGGTCACCGTCAt < 1:1478699/53‑1 (MQ=255)aGCTCGGTGTGACCCTTCTGGCGACCGAAACCCTTCACTTCGGTCACCGTCAt < 1:308007/53‑1 (MQ=255)aGCTCGGTGTGACCCTTCTGGCGACCGAAACCCTTCACTTCGGTCACCGTCAt < 1:392700/53‑1 (MQ=255)aGCTCGGTGTGACCCTTCTGGCGACCGAAACCCTTCACTTCGGTCACCGTCAt < 1:437192/53‑1 (MQ=255)aGCTCGGTGTGACCCTTCTGGCGACCGAAACCCTTCACTTCGGTCACCGTCAt < 1:795474/53‑1 (MQ=255)aGCTCGGTGCGACCCTTCTGGCGACCGAAACCCTTCACTTCGGTCACCGTCAt < 1:224051/53‑1 (MQ=255) | AGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCAT > minE/1719961‑1720013 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |