| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,787,031 | C→T | 100% | A140A (GCG→GCA) | yfjG ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,787,031 | 0 | C | T | 81.4% | 151.9 / 27.2 | 70 | A140A (GCG→GCA) | yfjG | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (7/6); new base T (0/57); total (7/63) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.43e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ACTGTAAACCTCTTTCGCACGAACCGTAAAAGCCTGGACCATATTAGCCGCCAGCTCTTTAAACACGCGACCAAAGG > minE/1786983‑1787059 | aCTGTAGACCTCTTTTGCACGAACAGTAAAAGCCTGCACCATATTAGCTGCCAGCTCTTTTAacac < 1:1539538/66‑1 (MQ=255)aCTGTAGACCTCTTTTGCACGAACAGTAAAAGCCTGCACCATATTAGCTGCCAGCTCTTTTAacac < 1:514262/66‑1 (MQ=255)aCTGTAGACCTCTTTTGCACGAACAGTAAAAGCCTGCACCATATTAGCTGCCAGCTCTTTAAacac < 1:325125/66‑1 (MQ=255)aCTGTAGACCTCTTTTGCACGAACAGTAAAAGCCTGCACCATATTAGCTGCCAGCTCTTTAAacac < 1:1793650/66‑1 (MQ=255)aCTGTAGACCTCTTTTGCACGAACAGTAAAAGCCTGCACCATATTAGCTGCCAGCTCTTTAAacac < 1:1863808/66‑1 (MQ=255)aCTGTAGACCTCTTTTGCACGAACAGTAAAAGCCTGCACCATATTAGCTGCCAGCTCTTTAAacac < 1:192549/66‑1 (MQ=255)aCTGTAGACCTCTTTTGCACGAACAGTAAAAGCCTGCACCATATTAGCTGCCAGCTCTTTAAacac < 1:1958119/66‑1 (MQ=255)aCTGTAGACCTCTTTTGCACGAACAGTAAAAGCCTGCACCATATTAGCTGCCAGCTCTTTAAacac < 1:1960269/66‑1 (MQ=255)aCTGTAGACCTCTTTTGCACGAACAGTAAAAGCCTGCACCATATTAGCTGCCAGCTCTTTAAacac < 1:2047416/66‑1 (MQ=255)aCTGTAGACCTCTTTTGCACGAACAGTAAAAGCCTGCACCATATTAGCTGCCAGCTCTTTAAacac < 1:213048/66‑1 (MQ=255)aCTGTAGACCTCTTTTGCACGAACAGTAAAAGCCTGCACCATATTAGCTGCCAGCTCTTTAAacac < 1:2137488/66‑1 (MQ=255)aCTGTAGACCTCTTTTGCACGAACAGTAAAAGCCTGCACCATATTAGCTGCCAGCTCTTTAAacac < 1:2222503/66‑1 (MQ=255)aCTGTAGACCTCTTTTGCACGAACAGTAAAAGCCTGCACCATATTAGCTGCCAGCTCTTTAAacac < 1:2277790/66‑1 (MQ=255)aCTGTAGACCTCTTTTGCACGAACAGTAAAAGCCTGCACCATATTAGCTGCCAGCTCTTTAAacac < 1:228116/66‑1 (MQ=255)aCTGTAGACCTCTTTTGCACGAACAGTAAAAGCCTGCACCATATTAGCTGCCAGCTCTTTAAacac < 1:239547/66‑1 (MQ=255)aCTGTAGACCTCTTTTGCACGAACAGTAAAAGCCTGCACCATATTAGCTGCCAGCTCTTTAAacac < 1:264746/66‑1 (MQ=255)aCTGTAGACCTCTTTTGCACGAACAGTAAAAGCCTGCACCATATTAGCTGCCAGCTCTTTAAacac < 1:2003298/66‑1 (MQ=255)aCTGTAGACCTCTTTTGCACGAACAGTAAAAGCCTGCACCATATTAGCTGCCAGCTCTTTAAacac < 1:422124/66‑1 (MQ=255)aCTGTAGACCTCTTTTGCACGAACAGTAAAAGCCTGCACCATATTAGCTGCCAGCTCTTTAAacac < 1:442011/66‑1 (MQ=255)aCTGTAGACCTCTTTTGCACGAACAGTAAAAGCCTGCACCATATTAGCTGCCAGCTCTTTAAacac < 1:546050/66‑1 (MQ=255)aCTGTAGACCTCTTTTGCACGAACAGTAAAAGCCTGCACCATATTAGCTGCCAGCTCTTTAAacac < 1:54963/66‑1 (MQ=255)aCTGTAGACCTCTTTTGCACGAACAGTAAAAGCCTGCACCATATTAGCTGCCAGCTCTTTAAacac < 1:569194/66‑1 (MQ=255)aCTGTAGACCTCTTTTGCACGAACAGTAAAAGCCTGCACCATATTAGCTGCCAGCTCTTTAAacac < 1:59215/66‑1 (MQ=255)aCTGTAGACCTCTTTTGCACGAACAGTAAAAGCCTGCACCATATTAGCTGCCAGCTCTTTAAacac < 1:61962/66‑1 (MQ=255)aCTGTAGACCTCTTTTGCACGAACAGTAAAAGCCTGCACCATATTAGCTGCCAGCTCTTTAAacac < 1:740977/66‑1 (MQ=255)aCTGTAGACCTCTTTTGCACGAACAGTAAAAGCCTGCACCATATTAGCTGCCAGCTCTTTAAacac < 1:800906/66‑1 (MQ=255)aCTGTAGACCTCTTTTGCACGAACAGTAAAAGCCTGCACCATATTAGCTGCCAGCTCTTTAAacac < 1:921180/66‑1 (MQ=255)aCTGTAGACCTCTTTTGCACGAACAGTAAAAGCCTGCACCATATTAGCTGCCAGCTCTTTAAacac < 1:992544/66‑1 (MQ=255)aCTGTAGACCTCTTTTGCACGAACAGTAAAAGCCTGCACCATATTAGCTGCCAGCTCTTTAAacac < 1:999673/66‑1 (MQ=255)aCTGTAGACCTCTTTTGCACGAACAGTAAAAGCCTGCACCATATTAGCTGCCAGCTCTTTAAacac < 1:1752231/66‑1 (MQ=255)aCTGTAGACCTCTTTTGCACGAACAGTAAAAGCCTGCACCATATTAGCTGCCAGCTCTTTAAacac < 1:1026454/66‑1 (MQ=255)aCTGTAGACCTCTTTTGCACGAACAGTAAAAGCCTGCACCATATTAGCTGCCAGCTCTTTAAacac < 1:1035251/66‑1 (MQ=255)aCTGTAGACCTCTTTTGCACGAACAGTAAAAGCCTGCACCATATTAGCTGCCAGCTCTTTAAacac < 1:1041797/66‑1 (MQ=255)aCTGTAGACCTCTTTTGCACGAACAGTAAAAGCCTGCACCATATTAGCTGCCAGCTCTTTAAacac < 1:1049534/66‑1 (MQ=255)aCTGTAGACCTCTTTTGCACGAACAGTAAAAGCCTGCACCATATTAGCTGCCAGCTCTTTAAacac < 1:1148543/66‑1 (MQ=255)aCTGTAGACCTCTTTTGCACGAACAGTAAAAGCCTGCACCATATTAGCTGCCAGCTCTTTAAacac < 1:1241939/66‑1 (MQ=255)aCTGTAGACCTCTTTTGCACGAACAGTAAAAGCCTGCACCATATTAGCTGCCAGCTCTTTAAacac < 1:1247767/66‑1 (MQ=255)aCTGTAGACCTCTTTTGCACGAACAGTAAAAGCCTGCACCATATTAGCTGCCAGCTCTTTAAacac < 1:1276729/66‑1 (MQ=255)aCTGTAGACCTCTTTTGCACGAACAGTAAAAGCCTGCACCATATTAGCTGCCAGCTCTTTAAacac < 1:1318054/66‑1 (MQ=255)aCTGTAGACCTCTTTTGCACGAACAGTAAAAGCCTGCACCATATTAGCTGCCAGCTCTTTAAacac < 1:1353431/66‑1 (MQ=255)aCTGTAGACCTCTTTTGCACGAACAGTAAAAGCCTGCACCATATTAGCTGCCAGCTCTTTAAacac < 1:1378289/66‑1 (MQ=255)aCTGTAGACCTCTTTTGCACGAACAGTAAAAGCCTGCACCATATTAGCTGCCAGCTCTTTAAacac < 1:1408853/66‑1 (MQ=255)aCTGTAGACCTCTTTTGCACGAACAGTAAAAGCCTGCACCATATTAGCTGCCAGCTCTTTAAacac < 1:14653/66‑1 (MQ=255)aCTGTAGACCTCTTTTGCACGAACAGTAAAAGCCTGCACCATATTAGCTGCCAGCTCTTTAAacac < 1:1469220/66‑1 (MQ=255)aCTGTAGACCTCTTTTGCACGAACAGTAAAAGCCTGCACCATATTAGCTGCCAGCTCTTTAAacac < 1:1610255/66‑1 (MQ=255)aCTGTAGACCTCTTTTGCACGAACAGTAAAAGCCTGCACCATATTAGCTGCCAGCTCTTTAAacac < 1:1777185/66‑1 (MQ=255)aCTGTAGACCTCTTTTGCACGAACAGTAAAAGCCTGCACCATATTAGCTGCCAGCTCTTTAAacac < 1:1747280/66‑1 (MQ=255)aCTGTAGACCTCTTTTGCACGAACAGTAAAAGCCTGCACCATATTAGCTGCCAGCTCTTTAAacac < 1:1713120/66‑1 (MQ=255)aCTGTAGACCTCTTTTGCACGAACAGTAAAAGCCTGCACCATATTAGCTGCCAGCTCTTTAAacac < 1:1482070/66‑1 (MQ=255)aCTGTAGACCTCTTTTGCACGAACAGTAAAAGCCTGCACCATATTAGCTGCCAGCTCTTTAAacac < 1:1555554/66‑1 (MQ=255)aCTGTAGACCTCTTTTGCACGAACAGTAAAAGCCTGCACCATATTAGCTGCCAGCTCTTTAAacac < 1:1539341/66‑1 (MQ=255)aCTGTAGACCTCTTTTGCACGAACAGTAAAAGCCTGCACCATATTAGCTGCCAGCTCTTTAAacac < 1:1489793/66‑1 (MQ=255)aCTGTAGACCTCTTTTGCACGAACAGTAAAAGCCTGCACCATATTAGCTGCCACCTCTTTAAacac < 1:2158393/66‑1 (MQ=255)aCTGTAGACCTCTTTTGCACGAACAGTAAAAGCCTGCACCATATTAGCTGCCACCTCTTTAAacac < 1:2155498/66‑1 (MQ=255)aCTGTAGACCTCTCTTGCACGAACAGTAAAAGCCTGCACCATATTAGCTGCCAGCTCTTTAAacac < 1:2040644/66‑1 (MQ=255)aCTGTAGACCTATTTTGCACGAACAGTAAAAGCCTGCACCATATTAGCTGCCAGCTCTTTAAacac < 1:563342/66‑1 (MQ=255) cTGTAGACCTCTTTTGCACGAACAGTAAAAGCCTGCACCATATTAGCTGCCAGCTCTTTAAacac < 1:1673092/65‑1 (MQ=255) ctTTCGCACGAACCGTAAAAGCCTGGACCATATTAGCCGCCAGctc > 1:1384653/1‑46 (MQ=255) ctTTCGCACGAACCGTAAAAGCCTGGACCATATTAGCCGCCAGctc > 1:32106/1‑46 (MQ=255) ctTTCGCACGAACCGTAAAAGCCTGGACCATATTAGCCGCCAGctc > 1:52638/1‑46 (MQ=255) ctTTCGCACGAACCGTAAAAGCCTGGACCATATTAGCCGCCAGctc > 1:544501/1‑46 (MQ=255) ctTTCGCACGAACCGTAAAAGCCTGGACCATATTAGCCGCCAGctc > 1:1279417/1‑46 (MQ=255) ctTTCGCACGAACCGTAAAAGCCTGGACCATATTAGCCGCCAGctc > 1:2292818/1‑46 (MQ=255) ctTTCGCACGAACCGTAAAAGCCTGGACCATATTAGCCGCCAGctc > 1:1038116/1‑46 (MQ=255) aaaGCCTGGACCATATTAGCCGCCAGCTCTTTAAACACGCGACCAAAgg < 1:2284936/49‑1 (MQ=255) aaaGCCTGGACCATATTAGCCGCCAGCTCTTTAAACACGCGACCAAAgg < 1:904602/49‑1 (MQ=255) aaaGCCTGGACCATATTAGCCGCCAGCTCTTTAAACACGCGACCAAAgg < 1:663258/49‑1 (MQ=255) aaaGCCTGGACCATATTAGCCGCCAGCTCTTTAAACACGCGACCAAAgg < 1:633377/49‑1 (MQ=255) aaaGCCTGGACCATATTAGCCGCCAGCTCTTTAAACACGCGACCAAAgg < 1:1665538/49‑1 (MQ=255) aaaGCCTGGACCATATTAGCCGCCAGCTCTTTAAACACGCGACCAAAgg < 1:1011148/49‑1 (MQ=255) | ACTGTAAACCTCTTTCGCACGAACCGTAAAAGCCTGGACCATATTAGCCGCCAGCTCTTTAAACACGCGACCAAAGG > minE/1786983‑1787059 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |