Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | minE | 1,797,368 | G→A | 100% | L234L (TTG→TTA) | nrdF → | ribonucleoside‑diphosphate reductase 2, beta subunit, ferritin‑like |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | minE | 1,797,368 | 0 | G | A | 100.0% | 123.4 / NA | 36 | L234L (TTG→TTA) | nrdF | ribonucleoside‑diphosphate reductase 2, beta subunit, ferritin‑like |
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (0/36); total (0/36) | |||||||||||
Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
ACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGC > minE/1797358‑1797421 | aCAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc < 1:622988/64‑1 (MQ=255) aCAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc < 1:1070199/64‑1 (MQ=255) aCAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc < 1:261868/64‑1 (MQ=255) aCAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc < 1:26308/64‑1 (MQ=255) aCAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc < 1:268957/64‑1 (MQ=255) aCAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc < 1:277343/64‑1 (MQ=255) aCAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc < 1:318518/64‑1 (MQ=255) aCAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc < 1:319594/64‑1 (MQ=255) aCAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc < 1:371730/64‑1 (MQ=255) aCAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc < 1:2231063/64‑1 (MQ=255) aCAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc < 1:649492/64‑1 (MQ=255) aCAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc < 1:677391/64‑1 (MQ=255) aCAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc < 1:754013/64‑1 (MQ=255) aCAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc < 1:77695/64‑1 (MQ=255) aCAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc < 1:78291/64‑1 (MQ=255) aCAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc < 1:853039/64‑1 (MQ=255) aCAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc < 1:871660/64‑1 (MQ=255) aCAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc < 1:92204/64‑1 (MQ=255) aCAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc < 1:1625349/64‑1 (MQ=255) aCAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc < 1:107092/64‑1 (MQ=255) aCAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc < 1:1130280/64‑1 (MQ=255) aCAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc < 1:1315880/64‑1 (MQ=255) aCAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc < 1:1343398/64‑1 (MQ=255) aCAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc < 1:1383833/64‑1 (MQ=255) aCAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc < 1:1430726/64‑1 (MQ=255) aCAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc < 1:1546149/64‑1 (MQ=255) aCAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc < 1:2210840/64‑1 (MQ=255) aCAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc < 1:1657773/64‑1 (MQ=255) aCAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc < 1:1683443/64‑1 (MQ=255) aCAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc < 1:1696037/64‑1 (MQ=255) aCAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc < 1:1941903/64‑1 (MQ=255) aCAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc < 1:2155043/64‑1 (MQ=255) aCAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc < 1:2159649/64‑1 (MQ=255) cAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc < 1:1790688/63‑1 (MQ=255) cAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc < 1:1905043/63‑1 (MQ=255) cAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc < 1:904118/63‑1 (MQ=255) | ACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGC > minE/1797358‑1797421 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |