Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,797,368 G→A 100% L234L (TTG→TTA nrdF → ribonucleoside‑diphosphate reductase 2, beta subunit, ferritin‑like

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,797,3680GA100.0% 123.4 / NA 36L234L (TTG→TTAnrdFribonucleoside‑diphosphate reductase 2, beta subunit, ferritin‑like
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (0/36);  total (0/36)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGC  >  minE/1797358‑1797421
          |                                                     
aCAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc  <  1:622988/64‑1 (MQ=255)
aCAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc  <  1:1070199/64‑1 (MQ=255)
aCAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc  <  1:261868/64‑1 (MQ=255)
aCAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc  <  1:26308/64‑1 (MQ=255)
aCAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc  <  1:268957/64‑1 (MQ=255)
aCAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc  <  1:277343/64‑1 (MQ=255)
aCAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc  <  1:318518/64‑1 (MQ=255)
aCAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc  <  1:319594/64‑1 (MQ=255)
aCAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc  <  1:371730/64‑1 (MQ=255)
aCAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc  <  1:2231063/64‑1 (MQ=255)
aCAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc  <  1:649492/64‑1 (MQ=255)
aCAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc  <  1:677391/64‑1 (MQ=255)
aCAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc  <  1:754013/64‑1 (MQ=255)
aCAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc  <  1:77695/64‑1 (MQ=255)
aCAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc  <  1:78291/64‑1 (MQ=255)
aCAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc  <  1:853039/64‑1 (MQ=255)
aCAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc  <  1:871660/64‑1 (MQ=255)
aCAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc  <  1:92204/64‑1 (MQ=255)
aCAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc  <  1:1625349/64‑1 (MQ=255)
aCAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc  <  1:107092/64‑1 (MQ=255)
aCAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc  <  1:1130280/64‑1 (MQ=255)
aCAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc  <  1:1315880/64‑1 (MQ=255)
aCAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc  <  1:1343398/64‑1 (MQ=255)
aCAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc  <  1:1383833/64‑1 (MQ=255)
aCAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc  <  1:1430726/64‑1 (MQ=255)
aCAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc  <  1:1546149/64‑1 (MQ=255)
aCAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc  <  1:2210840/64‑1 (MQ=255)
aCAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc  <  1:1657773/64‑1 (MQ=255)
aCAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc  <  1:1683443/64‑1 (MQ=255)
aCAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc  <  1:1696037/64‑1 (MQ=255)
aCAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc  <  1:1941903/64‑1 (MQ=255)
aCAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc  <  1:2155043/64‑1 (MQ=255)
aCAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc  <  1:2159649/64‑1 (MQ=255)
 cAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc  <  1:1790688/63‑1 (MQ=255)
 cAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc  <  1:1905043/63‑1 (MQ=255)
 cAACGAGTTACAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGc  <  1:904118/63‑1 (MQ=255)
          |                                                     
ACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGC  >  minE/1797358‑1797421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: