| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,875,977 | A→G | 100% | N262S (AAC→AGC) | ygcG → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,875,977 | 0 | A | G | 78.3% | 40.4 / 7.7 | 23 | N262S (AAC→AGC) | ygcG | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (5/0); new base G (0/18); total (5/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.97e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGC > minE/1875912‑1875989 | tATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGacaac > 1:1437501/1‑67 (MQ=255)tATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGacaac > 1:616098/1‑67 (MQ=255) cTTTACCTGGTTATGTGTGCCACAGAAAAAAGCATCAGGTAGAAGTTATCATTCAGACAGcggcgg < 1:1788942/66‑1 (MQ=255) cTTTACCTGGTTATGTGTCCCACAGAAATAAGCTTCAGGTAGAAGTTATCATTCAGACAGcggcgg < 1:1553180/66‑1 (MQ=255) cTTTACCTGGTTATGTGTCCCACAGAAAAAAGCATCAGGTAGAAGTTATCATTCAGACAGcggcgg < 1:788810/66‑1 (MQ=255) cTTTACCTGGTTATGTGTCCCACAGAAAAAAGCATCAGGTAGAAGTTATCATTCAGACAGcggcgg < 1:765318/66‑1 (MQ=255) cTTTACCTGGTTATGTGTCCCACAGAAAAAAGCATCAGGTAGAAGTTATCATTCAGACAGcggcgg < 1:586740/66‑1 (MQ=255) cTTTACCTGGTTATGTGTCCCACAGAAAAAAGCATCAGGTAGAAGTTATCATTCAGACAGcggcgg < 1:384727/66‑1 (MQ=255) cTTTACCTGGTTATGTGTCCCACAGAAAAAAGCATCAGGTAGAAGTTATCATTCAGACAGcggcgg < 1:380666/66‑1 (MQ=255) cTTTACCTGGTTATGTGTCCCACAGAAAAAAGCATCAGGTAGAAGTTATCATTCAGACAGcggcgg < 1:289973/66‑1 (MQ=255) cTTTACCTGGTTATGTGTCCCACAGAAAAAAGCATCAGGTAGAAGTTATCATTCAGACAGcggcgg < 1:2242060/66‑1 (MQ=255) cTTTACCTGGTTATGTGTCCCACAGAAAAAAGCATCAGGTAGAAGTTATCATTCAGACAGcggcgg < 1:2135429/66‑1 (MQ=255) cTTTACCTGGTTATGTGTCCCACAGAAAAAAGCATCAGGTAGAAGTTATCATTCAGACAGcggcgg < 1:2133107/66‑1 (MQ=255) cTTTACCTGGTTATGTGTCCCACAGAAAAAAGCATCAGGTAGAAGTTATCATTCAGACAGcggcgg < 1:2103686/66‑1 (MQ=255) cTTTACCTGGTTATGTGTCCCACAGAAAAAAGCATCAGGTAGAAGTTATCATTCAGACAGcggcgg < 1:1920379/66‑1 (MQ=255) cTTTACCTGGTTATGTGTCCCACAGAAAAAAGCATCAGGTAGAAGTTATCATTCAGACAGcggcgg < 1:1747079/66‑1 (MQ=255) cTTTACCTGGTTATGTGTCCCACAGAAAAAAGCATCAGGTAGAAGTTATCATTCAGACAGcggcgg < 1:1712766/66‑1 (MQ=255) cTTTACCTGGTTATGTGTCCCACAGAAAAAAGCATCAGGTAGAAGTTATCATTCAGACAGcggcgg < 1:166825/66‑1 (MQ=255) cTTTACCTGGTTATGTGTCCCACAGAAAAAAGCATCAGGTAGAAGTTATCATTCAGACAGcggcgg < 1:1486280/66‑1 (MQ=255) tttACCTGGTTATGTGTCCCACAGAAAAAAGCATCAGGTAGAAGTTATCATTCAGACAGcggcgg < 1:223426/65‑1 (MQ=255) ccTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGc > 1:479772/1‑67 (MQ=255) ccTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGc > 1:481823/1‑67 (MQ=255) ccTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGc > 1:484432/1‑67 (MQ=255) ccTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGc > 1:1473017/1‑67 (MQ=255) ccTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGc > 1:1239255/1‑67 (MQ=255) | TATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGC > minE/1875912‑1875989 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |