| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,892,249 | C→A | 100% | V211L (GTG→TTG) | ygdL ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,892,249 | 0 | C | A | 100.0% | 14.9 / NA | 6 | V211L (GTG→TTG) | ygdL | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (0/6); total (0/6) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CGTACCGTCTGACTGCGGGTACACCAGCGCTTCAGTAGAAAACACGCAATCCACGCCGAGCTTACCTTTACTGTTTTTCACTACGCCAA > minE/1892226‑1892314 | cgtaccgtCTGACTGCGGGTACAACAGCGCTTCAGTAGAAAACACGCAATCCACGCCGAGCTTACCt < 1:1568219/67‑1 (MQ=255)cgtaccgtCTGACTGCGGGTACAACAGCGCTTCAGTAGAAAACACGCAATCCACGCCGAGCTTACCt < 1:1734346/67‑1 (MQ=255)cgtaccgtCTGACTGCGGGTACAACAGCGCTTCAGTAGAAAACACGCAATCCACGCCGAGCTTACCt < 1:1824339/67‑1 (MQ=255)cgtaccgtCTGACTGCGGGTACAACAGCGCTTCAGTAGAAAACACGCAATCCACGCCGAGCTTACCt < 1:2009195/67‑1 (MQ=255)cgtaccgtCTGACTGCGGGTACAACAGCGCTTCAGTAGAAAACACGCAATCCACGCCGAGCTTACCt < 1:575831/67‑1 (MQ=255)cgtaccgtCTGACTGCGGGTACAACAGCGCTTCAGTAGAAAACACGCAATCCACGCCGAGCTTACCt < 1:676790/67‑1 (MQ=255) acCAGCGCTTCAGTAGAAAACACGCAATCCACGCCGAGCTTACCTTTACTGTTTTTCACTACGCCaa > 1:2192839/1‑67 (MQ=255) | CGTACCGTCTGACTGCGGGTACACCAGCGCTTCAGTAGAAAACACGCAATCCACGCCGAGCTTACCTTTACTGTTTTTCACTACGCCAA > minE/1892226‑1892314 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |