| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,914,848 | T→C | 100% | E208E (GAA→GAG) | ptsP ← | fused PEP‑protein phosphotransferase (enzyme I) of PTS system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,914,848 | 0 | T | C | 100.0% | 29.0 / NA | 10 | E208E (GAA→GAG) | ptsP | fused PEP‑protein phosphotransferase (enzyme I) of PTS system |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (10/0); total (10/0) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GGCGAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAG > minE/1914799‑1914865 | ggctAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGCTCCAGAGCCGGATCCAg > 1:841514/1‑67 (MQ=255)ggcgAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGCTCCAGCGCCGGATCCAg > 1:305327/1‑67 (MQ=255)ggcgAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGCTCCAGAGCCGGATCCAg > 1:1139690/1‑67 (MQ=255)ggcgAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGCTCCAGAGCCGGATCCAg > 1:1214613/1‑67 (MQ=255)ggcgAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGCTCCAGAGCCGGATCCAg > 1:144285/1‑67 (MQ=255)ggcgAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGCTCCAGAGCCGGATCCAg > 1:1790556/1‑67 (MQ=255)ggcgAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGCTCCAGAGCCGGATCCAg > 1:1838893/1‑67 (MQ=255)ggcgAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGCTCCAGAGCCGGATCCAg > 1:2099121/1‑67 (MQ=255)ggcgAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGCTCCAGAGCCGGATCCAg > 1:692802/1‑67 (MQ=255)ggcgAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGCTCCAGAGCCGGATCCAg > 1:770470/1‑67 (MQ=255) | GGCGAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAG > minE/1914799‑1914865 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |