| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 60,568 | G→A | 46.0% | R125R (CGC→CGT) | hepA ← | RNA polymerase‑associated helicase protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 60,568 | 0 | G | A | 46.0% | 11.2 / 65.9 | 50 | R125R (CGC→CGT) | hepA | RNA polymerase‑associated helicase protein |
| Reads supporting (aligned to +/- strand): ref base G (19/8); new base A (11/12); total (30/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.49e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCCGCTGTACGGCATACGGAACTGTTCGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAGCGGTCCATACGGTCAATCTGCCCGGCAAACAGACGGTCCTGCGGTTTGCTGAACACCAGTTTGCT > minE/60505‑60633 | gCCGCTGTACGGCATACGGAACTGTTCGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAGCGGTCCa < 1:2880633/71‑1 (MQ=255) cGCTGTACGGCATACGGAACTGTTCGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAGCGGTCCATa > 1:154489/1‑71 (MQ=255) cGCTGTACGGCATACGGAACTGTTCGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAGCGGTCCATa > 1:727923/1‑71 (MQ=255) ccTGTACGGCATACGGAACTGTTCGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAACGGTCCATAc > 1:2857088/2‑71 (MQ=255) tACGGCATACGGAACTGTTCGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAACGGTc < 1:2942707/62‑1 (MQ=255) aCGGCATACGGAACTGTTCGCTGGAATATTTACGCGCGGGATAACGCAGCGCAAAGCGGTCCATACGGTc > 1:2064756/1‑70 (MQ=255) aCGGCATACGGAACTGTTCGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAGCGGTCCATACGGt > 1:2910628/1‑69 (MQ=255) aCGGCATACGGAACTGTTCGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAGCGGTCCATACGGTc > 1:3101906/1‑70 (MQ=255) aCGGCATACGGAACTGTTCGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAGCGGTCCATACGGTc > 1:2994315/1‑70 (MQ=255) aCGGCATACGGAACTGTTCGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAGCGGTCCATACGGTc > 1:1462688/1‑70 (MQ=255) aCGGCATACGGAACTGTTCGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAGCGGTCCATACGGTc > 1:2470097/1‑70 (MQ=255) cGGCATACGGAACTGTTCGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAGCGGTCCATACGGTCa < 1:3500529/70‑1 (MQ=255) cGGCATACGGAACTGTTCGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAGCGGTCCATACGGTCa < 1:1312672/70‑1 (MQ=255) cGGCATACGGAACTGTTCGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAACGGTCCATACGGTCaa < 1:1119029/71‑1 (MQ=255) cGGCATACGGAACTGTTCGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAACGGTCCATACGGTCaa < 1:2407305/71‑1 (MQ=255) cGGCATACGGAACTGTTCGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAACGGTCCATACGGTCaa > 1:1324110/1‑71 (MQ=255) tACGGAACTGTTCGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAGCGGTCCATACGGTCAATCTGc > 1:3535222/1‑71 (MQ=255) tACGGAACTGTTCGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAGCGGTCCATACGGTCAATCTGc > 1:1657259/1‑71 (MQ=255) cGGAACTGTTCGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAGCGGTCCATACGGTCAATCTGccc > 1:3794265/1‑71 (MQ=255) cGGAACTGTTCGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAGCGGTCCATACGGTCAATCTGccc > 1:3809005/1‑71 (MQ=255) cGGAACTGTTCGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAGCGGTCCATACGGTCAATCTGccc > 1:861999/1‑71 (MQ=255) cGGAACTGTTCGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAGCGGTCCATACGGTCAATCTGcac > 1:801746/1‑69 (MQ=255) cGGAACTGTTCGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAACGGTCCATACGGTCAATCTGccc > 1:2088791/1‑71 (MQ=255) cGGAACTGTTCGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAACGGTCCATACGGTCAATCTGccc > 1:362504/1‑71 (MQ=255) cGGAACTGTTCGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAACGGTCCATACGGTCAATCTGccc > 1:2038499/1‑71 (MQ=255) aaCTGTTCGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAACGGTc < 1:823220/50‑1 (MQ=255) aCTGTTCGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAACGGTCCATACGGTCAATCTGCCCGGCa < 1:132161/71‑1 (MQ=255) aCTGTTCGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAACGGTCCATACGGTCAATCTGCCCGGCa < 1:3443404/71‑1 (MQ=255) cTGTTCGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAACGGTCCATACGGTCaa < 1:3534315/59‑1 (MQ=255) cTGTTCGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAACGGTCCATACGGTCaa < 1:2266357/59‑1 (MQ=255) cGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAACGGTCCATACGGTCAATCTGCCCGGCAAACAGa > 1:3127165/1‑71 (MQ=255) cGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAACGGTCCATACGGTCAATCTGCCCGGCAAACAGa > 1:2092263/1‑71 (MQ=255) gAATATTTACGCGCGCGATAACGCAGCGCAAAACGGTCCATACGGTCAATCTGCCCGGCAAACAGACgg < 1:1736853/69‑1 (MQ=255) tttACGCGCGCGATAACGCAGCGCAAAACGGTCCATACGGTCAATCTGCCCGGCAAACAGACGGTCCTGCg < 1:1636181/71‑1 (MQ=255) tttACGCGCGCGATAACGCAGCGCAAAACGGTCCATACGGTCAATCTGCCCGGCAAACAGACGGTCCTGCg < 1:677243/71‑1 (MQ=255) ttACGCGCGCGATAACGCAGCGCAAAACGGTCCATACGGTCAATCTGCCCGGCAAACAGACGGTCCTGCgg > 1:640213/1‑71 (MQ=255) ttACGCGCGCGATAACGCAGCGCAAAACGGTCCATACGGTCAATCTGCCCGGCAAACAGACGGTCCTGCgg > 1:1935350/1‑71 (MQ=255) cgcgATAACGCAGCGCAAAACGGTCCATACGGTCAATCTGCCCGGCAAACAGACGGt < 1:3603185/57‑1 (MQ=255) cgATAACGCAGCGCAAAACGGTCCATACGGTCAAt > 1:335879/1‑35 (MQ=255) cgATAACGCAGCGCAAAACGGTCCATACGGTCAATCTGCCCGGCAAACAGACGGTCCTGCGGTTTGCTGaa > 1:118023/1‑71 (MQ=255) aCGCAGCGCAAAGCGGTCCATACGGTCAATCTGCCCGGCAAACAGACGGTCCTGCGGTTTGCTGaa > 1:659271/1‑66 (MQ=255) aCGCAGCGCAAAGCGGTCCATACGGTCAATCTGCCCGGCAAACAGACGGTCCTGCGGTTTGCTGaa > 1:1268821/1‑66 (MQ=255) aCGCAGCGCAAAGCGGTCCATACGGTCAATCTGCCCGGCAAACAGACGGTCCTGCGGTTTGCTGaa > 1:917587/1‑66 (MQ=255) aCGCAGCGCAAAGCGGTCCATACGGTCAATCTGCCCGGCAAACAGACGGTCCTGCGGTTTGCTGAacagaa > 1:151164/1‑68 (MQ=255) aCGCAGCGCAAAGCGGACCATAAGCTCAATCTGCCCGGCAAACAGACGGt > 1:3674938/1‑50 (MQ=255) gcAAAGCGGTCCATACGGTCAATCTGCCCGGCAAACAGACGGTCCTGCGGTTTGCTGAACACCAGTTTGCt < 1:2801426/71‑1 (MQ=255) gcAAAGCGGTCCATACGGTCAATCTGCCCGGCAAACAGACGGTCCTGCGGTTTGCTGAACACCAGTTTGCt < 1:1278601/71‑1 (MQ=255) cAAAGCGGTCCATACGGTCAATCTGCCCGGCAAACAGACGGTCCTGCGGTTTGc < 1:3378488/54‑1 (MQ=255) cAAAGCGGTCCATACGGTCAATCTGCCCGGCAAACAGACGGTCCTGCGGTTTGc < 1:251402/54‑1 (MQ=255) cAAAGCGGTCCATACGGTCAATCTGCCCGGCAAACAGACGGTCCTGCGGTTTGc < 1:1982749/54‑1 (MQ=255) | GCCGCTGTACGGCATACGGAACTGTTCGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAGCGGTCCATACGGTCAATCTGCCCGGCAAACAGACGGTCCTGCGGTTTGCTGAACACCAGTTTGCT > minE/60505‑60633 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |