| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,531,609 | A→G | 24.0% | I14I (ATT→ATC) | coaD ← | pantetheine‑phosphate adenylyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,531,609 | 0 | A | G | 24.0% | 138.3 / 47.3 | 75 | I14I (ATT→ATC) | coaD | pantetheine‑phosphate adenylyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (24/33); new base G (2/16); total (26/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.19e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01 | |||||||||||
GCCAGAATAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTCAATGCGTTTTCGGTGGCA > minE/2531547‑2531677 | gacAGAATAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATc > 1:1902855/3‑71 (MQ=255)gCCAGAATAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGTGATc > 1:1556255/1‑71 (MQ=255)gCCAGAATAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGAt > 1:1672744/1‑70 (MQ=255)gCCAGAATAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATc > 1:3415800/1‑71 (MQ=255)gCCAGAATAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATc > 1:1561243/1‑71 (MQ=255) cAGAATAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGa < 1:2411482/71‑1 (MQ=255) cAGAATAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGa < 1:2371389/71‑1 (MQ=255) cAGAATAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGa < 1:3392291/71‑1 (MQ=255) cAGAATAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGa < 1:3809034/71‑1 (MQ=255) cAGAATAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGa < 1:3423360/71‑1 (MQ=255) cAGAATAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGa < 1:3438025/71‑1 (MQ=255) cAGAATAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGa < 1:3757157/71‑1 (MQ=255) cAGAATAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGa < 1:278756/71‑1 (MQ=255) cAGAATAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGa < 1:3121255/71‑1 (MQ=255) gAATAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGaa < 1:3705385/70‑1 (MQ=255) gAATAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGaa < 1:2254049/70‑1 (MQ=255) gAATAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGaa < 1:3078958/70‑1 (MQ=255) gAATAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGaa < 1:536904/70‑1 (MQ=255) gAATAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGa < 1:1640663/69‑1 (MQ=255) gAATAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGa < 1:3196299/69‑1 (MQ=255) aaTAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGAAAg < 1:951877/71‑1 (MQ=255) aaTAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAg < 1:3808997/71‑1 (MQ=255) aaTAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAg < 1:383703/71‑1 (MQ=255) aaTAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAg < 1:2138914/71‑1 (MQ=255) aaTAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAg < 1:332216/71‑1 (MQ=255) aaTAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAg < 1:448639/71‑1 (MQ=255) aTAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGaaa > 1:3798511/1‑69 (MQ=255) aTAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGaaa > 1:3106751/1‑69 (MQ=255) aTAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGaaa > 1:1122306/1‑69 (MQ=255) aTAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGaaa > 1:1941679/1‑69 (MQ=255) aTAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGt < 1:550391/71‑1 (MQ=255) tAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGa < 1:1593202/66‑1 (MQ=255) aCGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGa < 1:4005919/64‑1 (MQ=255) cGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTaa > 1:503985/1‑48 (MQ=255) cGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGAATCGAAAGTACCCGGATa > 1:4053039/1‑70 (MQ=255) gAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGAAAGTACCCGGATaa > 1:439961/1‑70 (MQ=255) cATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAAt > 1:253011/1‑69 (MQ=255) cATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAAt > 1:1403806/1‑69 (MQ=255) cATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAAt > 1:3151919/1‑69 (MQ=255) tCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGa < 1:1516068/51‑1 (MQ=255) tCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGa < 1:7840/51‑1 (MQ=255) tGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCg > 1:2258684/1‑71 (MQ=255) tGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCg > 1:1002033/1‑71 (MQ=255) tGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCg > 1:3993322/1‑71 (MQ=255) cGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGtt < 1:3448548/71‑1 (MQ=255) tGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGtttt < 1:3427920/71‑1 (MQ=255) ggCGCGCGTCACGATATCGATATGACCATTGGTaa > 1:3701942/1‑35 (MQ=255) gcgcgcGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTg < 1:2100634/71‑1 (MQ=255) gcgcgcGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTg < 1:874319/71‑1 (MQ=255) gcgcgcGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTg < 1:3537027/71‑1 (MQ=255) cgTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATa > 1:236997/1‑70 (MQ=255) tCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATaa < 1:2729264/50‑1 (MQ=255) tCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATaa < 1:2189321/50‑1 (MQ=255) tCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATaa < 1:264368/50‑1 (MQ=255) tCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATaa < 1:3998121/50‑1 (MQ=255) cACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATaa < 1:2349042/49‑1 (MQ=255) cgatatcgatatGACCATTGGTGATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAAcc < 1:1432741/71‑1 (MQ=255) cgatatcgatatGACCATTGGTGATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAAcc < 1:2218775/71‑1 (MQ=255) gatatcgatatGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCt < 1:2546353/71‑1 (MQ=255) gatatcgatatGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCt < 1:2119231/71‑1 (MQ=255) gatatcgatatGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCt < 1:3144935/71‑1 (MQ=255) tatGACCATTGGTGATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTCAATGCGt < 1:2734720/71‑1 (MQ=255) aCCATTGGTAATGGGATCGAAAGTACCCGGATAAAt > 1:1286116/1‑36 (MQ=255) aCCATTGGTAATGGGATCGAAAGTACCCGGATAAAt > 1:2555041/1‑36 (MQ=255) aCCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGttt > 1:1652642/1‑45 (MQ=255) aCCATTGGTAATGGGATCGAAAATACCCGGATAAAt > 1:3289777/1‑36 (MQ=255) cATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTCAATGCGTTTTCgg < 1:3842631/71‑1 (MQ=255) ttGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTCAATGCGTTTTCGGTg < 1:2802686/71‑1 (MQ=255) ttGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTCAATGCGTTTTCGGTg < 1:2468972/71‑1 (MQ=255) ggTGATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTCAATGCGTTTTCGGTgg > 1:2020792/1‑70 (MQ=255) ggTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATa > 1:2979884/1‑46 (MQ=255) ggTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATa > 1:3554480/1‑46 (MQ=255) ggTAATGGGATCGAAAGTACCCGGATAAATCGCCAGTTTTTGCATa > 1:984185/1‑46 (MQ=255) gTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTCAATGCGTTTTCGGTGGCa < 1:2721507/71‑1 (MQ=255) gTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTCAATGCGTTTTCGGTGGCa < 1:1204361/71‑1 (MQ=255) tAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCa < 1:555529/42‑1 (MQ=255) tAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCa < 1:1776618/42‑1 (MQ=255) | GCCAGAATAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTCAATGCGTTTTCGGTGGCA > minE/2531547‑2531677 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |