| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,540,373 | G→T | 24.9% | D300Y (GAT→TAT) | rfaJ → | UDP‑D‑glucose:(galactosyl)lipopolysaccharide glucosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,540,373 | 0 | G | T | 24.9% | 254.0 / 49.5 | 100 | D300Y (GAT→TAT) | rfaJ | UDP‑D‑glucose:(galactosyl)lipopolysaccharide glucosyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (37/38); new base T (14/11); total (51/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.47e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01 | |||||||||||
TATCCATCGGTAAAATACTATAAAATAGCTTTAGAAAATTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTAGTGCAACATCATTATATCT > minE/2540307‑2540439 | tATCCATCGGTAAAATACTATAAAATAGCTTTAGAAAATTCCCCCTGGAAAGATGACTCTCCACGATATGc > 1:513026/1‑71 (MQ=255)tATCCATCGGTAAAATACTATAAAATAGCTTTAGAAAATTCCCCCTGGAAAGATGACTCTCCACGATATGc > 1:132201/1‑71 (MQ=255)tATCCATCGGTAAAATACTATAAAATAGCTTTAGAAAATTCCCCCTGGAAAGATGACTCTCCACGAGATGc > 1:3648802/1‑71 (MQ=255) aTCCATCGGTAAAATACTATAAAATAGCTTTAGAAAATTCCCCCTGGAAAGATGACTCTCCACGAGATGc > 1:748756/1‑70 (MQ=255) gTAAAATACTATAAAATAGCTTTAGAAAATTCCCCCTGGAAAGATGACTCTCCACGATATGCGAAATCAat < 1:2049874/71‑1 (MQ=255) aaaTACTATAAAATAGCTTTAGAAAATTCCCCCTGGAAAGATGACTCTCCACGAGATGCGaaa > 1:3282430/1‑63 (MQ=255) aaaTACTATAAAATAGCTTTAGAAAATTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCAattat > 1:2337842/1‑71 (MQ=255) aTACTATAAAATAGCTTTAGAAAATTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCAATTATTg < 1:548684/71‑1 (MQ=255) aTACTATAAAATAGCTTTAGAAAATTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCAATTATTg < 1:236384/71‑1 (MQ=255) tACTATAAAATAGCTTTAGAAAATTCCCCATGGAAAGATGACTCTCCACGAGATGCGAAATCAATTATTg > 1:258155/1‑70 (MQ=255) ataAAATAGCTTTAGAAAATTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGAAtt > 1:190570/1‑70 (MQ=255) taAAATAGCTTTAGAAAATTCCCCCTGGAAAGATGACTCTCCACGATATGCGAAATCAATTATTGAATTTa < 1:2875278/71‑1 (MQ=255) taAAATAGCTTTAGAAAATTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGAAttt > 1:1649727/1‑70 (MQ=255) taAAATAGCTTTAGAAAATTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGAAttt > 1:204592/1‑70 (MQ=255) taAAATAGCTTTAGAAAATTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGAAttt > 1:1089631/1‑70 (MQ=255) aaaaTAGCTTTAGAAAATTCCCCCTGGAAAGATGACTCTCCACGATATGCGAAATCAATTATTGAATTTa > 1:2192964/1‑70 (MQ=255) aaaaTAGCTTTAGAAAATTCCCCCTGGAAAGATGACTCTCCACGATATGCGAAATCAATTATTGAATTTa > 1:1894483/1‑70 (MQ=255) aGCTTTAGAAAATTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGAATTTaaaaa > 1:1243544/1‑69 (MQ=255) gCTTTAGAAAATTCCCCCTGGAAAGATGACTCTCCACGATATGCGAAATCAATTATTGAATTTAAAAAAAg < 1:566678/71‑1 (MQ=255) gCTTTAGAAAATTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAg < 1:488526/71‑1 (MQ=255) gCTTTAGAAAATTCCCCCTGGAAAGATGAATCTCCACGATATGCGAAATCAATTATTGAATTTAAAAAAAg < 1:2447957/71‑1 (MQ=255) tttAGAAAATTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCAATTATTg < 1:3518207/56‑1 (MQ=255) tAGAAAATTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGata > 1:3399451/1‑70 (MQ=255) tAGAAAATTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGata < 1:2294622/70‑1 (MQ=255) aGAAAATTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCAat < 1:839580/48‑1 (MQ=255) aGAAAATTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCAat < 1:3284/48‑1 (MQ=255) aGAAAATTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCAat < 1:1492269/48‑1 (MQ=255) aGAAAATTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCAat < 1:2786465/48‑1 (MQ=255) gAAAATTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCaa > 1:1679441/1‑46 (MQ=255) gAAAATTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGAtata < 1:3479621/70‑1 (MQ=255) aaaaTTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATc < 1:3785279/43‑1 (MQ=255) aaaaTTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATc < 1:3355752/43‑1 (MQ=255) aaaaTTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATc < 1:1299234/43‑1 (MQ=255) aaaaTTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATc < 1:1458166/43‑1 (MQ=255) aaaaTTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATc < 1:803407/43‑1 (MQ=255) aaaaTTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATc < 1:1311409/43‑1 (MQ=255) aaTTCCCCCTGGAAAGATGACTCTCCACGATATGCGAAATCAATTATTGAATTTa < 1:1820997/55‑1 (MQ=255) aaTTCCCCCTGGAAAGATGACTCTCCACGATATGCGAAATCAATTATTGAATTTAAAAAAAg < 1:867508/62‑1 (MQ=255) aaTTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACa < 1:3314442/71‑1 (MQ=255) aTTCCCCCTGGATAGATGACTCTCCACGAGATGCGAAATCAat > 1:2326702/1‑43 (MQ=255) aTTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCAat > 1:3565020/1‑43 (MQ=255) aTTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCAat > 1:2931303/1‑43 (MQ=255) aTTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCAat > 1:3008180/1‑43 (MQ=255) aTTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCAat > 1:3389701/1‑43 (MQ=255) aTTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCAat > 1:524124/1‑43 (MQ=255) aTTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCAat > 1:1458802/1‑43 (MQ=255) ttCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATc < 1:3428122/71‑1 (MQ=255) ttCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATc < 1:652252/71‑1 (MQ=255) tCCCCCTGGAAAGATGACTCTCCACGATATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCt > 1:3458611/1‑71 (MQ=255) cTGGAAAGATGACTCTCCACGAGATGCGAAATCAatt < 1:3215782/37‑1 (MQ=255) cTGGAAAGATGACTCTCCACGAGATGCGAAATCAatt < 1:1500955/37‑1 (MQ=255) cTGGAAAGATGACTCTCCACGAGATGCGAAATCAat < 1:1953263/36‑1 (MQ=255) gAAAGATGACTCTCCACGATATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTAGTg > 1:3539554/1‑70 (MQ=255) gAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGAATTTaaaaaaa > 1:3797228/1‑50 (MQ=255) gAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTa < 1:3403608/67‑1 (MQ=255) gAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTAGTGc < 1:3989410/71‑1 (MQ=255) gAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTAGTGc < 1:3896009/71‑1 (MQ=255) gAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTAGTGc < 1:563749/71‑1 (MQ=255) gAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTAGTGc < 1:2158205/71‑1 (MQ=255) gAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTAGTGc < 1:3405384/71‑1 (MQ=255) gAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTAGTGc < 1:833412/71‑1 (MQ=255) aGATGACTCTCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTAGTGCaa > 1:2485461/1‑70 (MQ=255) gATGACTCTCCACGATATGCGAAATCAATTATTGAATTTAAAAAAAGa < 1:3446582/48‑1 (MQ=255) gATGACTCTCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTAGTGCAAca > 1:3919394/1‑71 (MQ=255) aTGACTTTCCACGAGATGCGAAATCAATTATTGaa > 1:3560927/1‑35 (MQ=255) aTGACTCTCCACGAGATGCGAAATCAATTATTGaa > 1:4046759/1‑35 (MQ=255) aTGACTCTCCACGAGATGCGAAATCAATTATTGaa > 1:934406/1‑35 (MQ=255) aTGACTCTCCACGAGATGCGAAATCAATTATTGaa > 1:343720/1‑35 (MQ=255) aTGACTCTCCACGAGATGCGAAATCAATTATTGaa > 1:2488755/1‑35 (MQ=255) aTGACTCTCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTAGTGCAAca < 1:1552045/70‑1 (MQ=255) tGACTCTCCACGATATGCGAAATCAATTATTGAATTTAAAAAAAGata > 1:1963676/1‑48 (MQ=255) gACTCTCCACGATATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTAGTGCAAcatca > 1:1693763/1‑71 (MQ=255) gACTCTCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTAGTGCAAcatca > 1:1553380/1‑71 (MQ=255) gACTCTCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTAGTGCAAcatca > 1:33733/1‑71 (MQ=255) aCTCTCCACGATATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTAGTGCAAcatcat < 1:1156930/71‑1 (MQ=255) aCTCTCCACGATATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTAGTGCAAcatcat < 1:3233143/71‑1 (MQ=255) aCTCTCCACGATATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTAGTGCAAcatcat > 1:3835892/1‑71 (MQ=255) aCTCTCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTAGTGCAAcatcat < 1:3067511/71‑1 (MQ=255) aCTCTCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTAGTGCAAcatcat < 1:2751716/71‑1 (MQ=255) ctctCCACGATATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACCTCTTTTACTGCAAcatcat > 1:440509/1‑70 (MQ=255) ctctCCACGATATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTAGTGCAACATCAtt < 1:1034828/71‑1 (MQ=255) ctctCCACGATATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTAGTGCAACATCAtt < 1:650159/71‑1 (MQ=255) ctctCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTa < 1:1702050/58‑1 (MQ=255) ctctCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTAGTGCAACATCAtt < 1:238846/71‑1 (MQ=255) ctcCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCttt < 1:1731326/54‑1 (MQ=255) ctcCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCttt < 1:287151/54‑1 (MQ=255) ctcCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCttt < 1:3703164/54‑1 (MQ=255) tcCACGATATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTAGTGCAACATCATtata > 1:3311433/1‑71 (MQ=255) tcCACGATATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTAGTGCAACATCATtata > 1:1772857/1‑71 (MQ=255) tcCACGATATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTAGTGCAACATCATta > 1:3522050/1‑69 (MQ=255) tcCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCt > 1:2454918/1‑51 (MQ=255) tcCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTa > 1:556020/1‑55 (MQ=255) tcCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTa > 1:2446643/1‑55 (MQ=255) tcCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTa > 1:114762/1‑55 (MQ=255) ccACGATATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTAGTGCAACATCATtata > 1:4016145/1‑70 (MQ=255) ccACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTa > 1:1280897/1‑54 (MQ=255) ccACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTa > 1:929393/1‑54 (MQ=255) ccACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTa > 1:2948846/1‑54 (MQ=255) cACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTAGTGCAACATCATTATAtc > 1:251374/1‑71 (MQ=255) aCGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTAGTGCAACATCATTATAtct < 1:3377991/71‑1 (MQ=255) | TATCCATCGGTAAAATACTATAAAATAGCTTTAGAAAATTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTAGTGCAACATCATTATATCT > minE/2540307‑2540439 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |