| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,572,558 | A→G | 6.2% | intergenic (+1326/‑453) | yibH → / → ysaB | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,572,558 | 0 | A | G | 6.2% | 311.3 / 10.5 | 112 | intergenic (+1326/‑453) | yibH/ysaB | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (76/29); new base G (4/3); total (80/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.05e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01 | |||||||||||
Only 100 of 123 total aligned reads displayed.
GCCATCTATTTATAAATGTGTACAACGGGGATTATTTTTACATTGCATATTATTACCAGAAGCGACTGGAATACTAAGCACATCATAAAAATTATCATCACATGGTATATCTGAAACACACGAAAGCATACCTAAAGTTA > minE/2572488‑2572627 | gCCATCTATTTATAAATGTGTACAACGGGGATTATTTTTACATTGCATATTATTACCAGAAGCGACTGGaa < 1:3579830/71‑1 (MQ=255) ccATCTATTTATAAATGTGTACAACGGGGATTATTTTTACATTGCATATTATTACCAGAAGCGACTGGaa > 1:2520183/1‑70 (MQ=255) tATTTATAAATGTGTACAACGGGGATTATTTTTACATTGCATATTATTACCAGAAGCGACTGGaa > 1:3574421/1‑65 (MQ=255) tATTTATAAATGTGTACAACGGGGATTATTTTTACATTGCATATTATTACCAGAAGCGACTGGaa > 1:1919270/1‑65 (MQ=255) tATTTATAAATGTGTACAACGGGGATTATTTTTACATTGCATATTATTACCAGAAGCGACTGGaa > 1:421611/1‑65 (MQ=255) tATTTATAAATGTGTACAACGGGGATTATTTTTACATTGCATATTATTACCAGAAGCGACTGGAGTACt > 1:571853/1‑69 (MQ=255) tATTTATAAATGTGTACAACGGGGATTATTTTTACATTGCATATTATTACCAGAAGCGACTGGAGTACt > 1:1484245/1‑69 (MQ=255) tATTTATAAATGTGTACAACGGGGATTATTTTTACATTGCATATTATTACCAGAAGCGACTGGAATACt > 1:2773236/1‑69 (MQ=255) tATTTATAAATGTGTACAACGGGGATTATTTTTACATTGCATATTATTACCAGAAGCGACTGGAATACt > 1:3504144/1‑69 (MQ=255) tATTTATAAATGTGTACAACGGGGATTATTTTTACATTGCATATTATTACCAGAAGCGACTGGAATACt > 1:4103727/1‑69 (MQ=255) tATTTATAAATGTGTACAACGGGGATTATTTTTACATTGCATATTATTACCAGAAGCGACTGGAATACt > 1:552280/1‑69 (MQ=255) tATTTATAAATGTGTACAACGGGGATTATTTTTACATTGCATATTATTACCAGAAGCGACTGGAATACt > 1:3381009/1‑69 (MQ=255) tATTTATAAATGTGTACAACGGGGATTATTTTTACATTGCATATTATTACCAGAAGCGACTGGAATACt > 1:3340045/1‑69 (MQ=255) tATTTATAAATGTGTACAACGGGGATTATTTTTACATTGCATATTATTACCAGAAGCGACTGGAATACt > 1:1169621/1‑69 (MQ=255) tATTTATAAATGTGTACAACGGGGATTATTTTTACATTGCATATTAATACCAGAAGCGACTGGAATACt > 1:7436/1‑69 (MQ=255) tataAATGTGTACAACGGGGATTATTTTTACATTGCATATTATTACCAGAAGCGACTGGAATACTAAGca > 1:545837/1‑70 (MQ=255) tataAATGTGTACAACGGGGATTATTTTTACATTGCATATTATTACCAGAAGCGACTGGAATACTAAGca > 1:4080831/1‑70 (MQ=255) ataAATGTGTACAACGGGGATTATTTTTACATTGCATATTATTACCAGAAGCGACTGGAATACTAAGcaca > 1:1998862/1‑71 (MQ=255) tgtgTACAACGGGGATTATTTTTACATTGCATATTATTACCAGAAGCGACTGGAATACTAAGCAcatca > 1:2733851/1‑69 (MQ=255) gtACAACGGGGATTGTTTTTACATTGCATATTATTACCAGAAGCGACTGGAATACTAAGCACATCATaaa > 1:3703292/1‑70 (MQ=255) gtACAACGGGGATTATTTTTACATTGCATATTATTACCAGAAGCGACTGGAATACTAAGCACATCATaaa > 1:412276/1‑70 (MQ=255) tACAACGGGGATTATTTTTACATTGCATATTATTACCAGAAGCGACTGGAATACTAAGCACATCATaaaa < 1:2127084/70‑1 (MQ=255) ggggATTATTTTTACATTGCATATTATTACCAGAAGCGACTGGAATACTAAGCACATCATAAAAATTatca > 1:3310837/1‑71 (MQ=255) ggggATTATTTTTACATTGCATATTATTACCAGAAGCGACTGGAATACTAAGCACATCATAAAAATTatca > 1:2953512/1‑71 (MQ=255) ggggATTATTTTTACATTGCATATTATTACCAGAAGCGACTGGAATACTAAGCACATCATAAAAATTatca < 1:2920164/71‑1 (MQ=255) ttattTTTACATTGCATATTATTACCAGAAGCGACTGGAATACTAAGCACATCATAAAAAtt < 1:853292/62‑1 (MQ=255) ttattTTTACATTGCATATTATTACCAGAAGCGACTGGAATACTAAGCACATCATAAAAAtt < 1:645121/62‑1 (MQ=255) attTTTACATTGCATATTATTACCAGAAGCGACTGGAATACTAAGCACATCATAAAAATTat > 1:787284/1‑62 (MQ=255) attTTTACATTGCATATTATTACCAGAAGCGACTGGAATACTAAGCACATCATAAAAATTat > 1:3012190/1‑62 (MQ=255) tttttACATTGCATATTATTACCAGAAGCGACTGGaa < 1:261891/37‑1 (MQ=255) tttttACATTGCATATTATTACCAGAAGCGACTGGAATACTAAGCACATCATAAAAATTATCATCACATgg > 1:3474771/1‑71 (MQ=255) tttACATTGCATATTATTACCAGAAGCGACTGGAGTACTAAGCACATCATaa > 1:3557629/1‑52 (MQ=255) tttACATTGCATATTATTACCAGAAGCGACTGGAGTACTAAGCACATCATaa > 1:2384618/1‑52 (MQ=255) tttACATTGCATATTATTACCAGAAGCGACTGGAATACTAAGCACATCATAAAAATTATCATCACATGGta > 1:3682822/1‑71 (MQ=255) tttACATTGCATATTATTACCAGAAGCGACTGGAATACTAAGCACATCATAAAAATTATCATCACATGGta > 1:3400340/1‑71 (MQ=255) aCATTGCATATTATTACCAGAAGCGACTGGAATACTAAGCACATCATAAAAATTATCATCACATGGtata < 1:1130594/70‑1 (MQ=255) tGCATATTATTACCAGAAGCGACTGGAATACTAAGCACATCATAAAAATTATCATCACATGGTATATCTg > 1:960539/1‑70 (MQ=255) gaatatTATTACCAGAAGCGACTGGAATACTAAGCACATCATAAAAATTAtcatca > 1:2064276/3‑56 (MQ=255) gCATATTATTACCAGAAGCGACTGGAATACTAAGCACATCATAAAAATTAtcatca > 1:1997283/1‑56 (MQ=255) gCATATTATTACCAGAAGCGACTGGAATACTAAGCACATCATAAAAATTAtcatca > 1:2318437/1‑56 (MQ=255) gCATATTATTACCAGAAGCGACTGGAATACTAAGCACATCATAAAAATTATCAT‑ACATGGTATATCTGaaa > 1:56067/1‑71 (MQ=255) cATATTATTACCAGAAGCGACTGGAATACTAAGCACATCATAAAAATTATCATCACATGGTATATCTGaaa < 1:1928345/71‑1 (MQ=255) atatTATTACCAGAAGCGACTGGAATACTAAGCACATCATAAAAATTATCATCACATGGTATATCTGaaa > 1:2356132/1‑70 (MQ=255) atatTATTACCAGAAGCGACTGGAATACTAAGCACATCATAAAAATTATCATCACATGGTATATCTGaa > 1:1183985/1‑69 (MQ=255) attattACCAGAAGCGACTGGAATACTAAGCACATCATAAAAATTatcatc < 1:1778906/51‑1 (MQ=255) ttattaCCAGAAGCGACTGGAATACTAAGCACATCATAAAAATTat > 1:992190/1‑46 (MQ=255) tattaCCAGAAGCGACTGGAATACTAAGCACATCATAAAAATTATCATcaca > 1:677156/1‑52 (MQ=255) tattaCCAGAAGCGACTGGAATACTAAGCACATCATAAAAATTATCATCACATGGTATATCTGAAacacac > 1:1385989/1‑71 (MQ=255) tattaCCAGAAGCGACTGGAATACTAAGCACATCATAAAAATTATCATCACATGGTATATCTGAAacacac > 1:2642483/1‑71 (MQ=255) tattaCCAGAAGCGACTGGAATACTAAGCACATCATAAAAATTATCATCACATGGTATATCTGAAacacac > 1:3244285/1‑71 (MQ=255) tattaCCAGAAGCGACTGGAATACTAAGCACATCATAAAAATTATCATCACATGGTATATCTGAAacaca > 1:3857787/1‑70 (MQ=255) attaCCAGAAGCGACTGGAATACTAAGCACATCATAAAAATTATCATCACATGGTATATCTGAAacacac > 1:1606411/1‑70 (MQ=255) attaCCAGAAGCGACTGGAATACTAAGCACATCATAAAAATTATCATCACATGGTATATCTGAAacaca > 1:1748777/1‑69 (MQ=255) aCCAGAAGCGACTGGAATACTAAGCACATCATAAAAATTATCATCACATGGTATATCTGAAACACACGa > 1:2271919/1‑69 (MQ=255) aCCAGAAGCGACTGGAATACTAAGCACATCATAAAAATTATCATCACATGGTATATCTGAAACACACGa > 1:3541603/1‑69 (MQ=255) aCCAGAAGCGACTGGAATACTAAGCACATCATAAAAATTATCATCACATGGTATATCTGAAACACACGa > 1:4048192/1‑69 (MQ=255) aCCAGAAGCGACTGGAATACTAAGCACATCATAAAAATTATCATCACATGGTATATCTGAAACACACGa > 1:401572/1‑69 (MQ=255) aCCAGAAGCGACTGGAATACTAAGAACATCATAAAAATTATCATCACATGGTATATCTGAAACACACGa > 1:1550663/1‑69 (MQ=255) ccAGAAGCGACTGGAATACTAAGCACATCATAAAAATTATCATCACATGGTATATCTGAAACACACGAAAg < 1:2544334/71‑1 (MQ=255) ccAGAAGCGACTGGAATACTAAGCACATCATAAAAATTATCATCACATGGTATATCTGAAACACACGAAAg < 1:2090178/71‑1 (MQ=255) ccAGAAGCGACTGGAATACTAAGCACATCATAAAAATTATCATCACATGGTATATCTGAAACACACGAAAg < 1:1378146/71‑1 (MQ=255) cAGAAGCGACTGGAATACTAAGCACATCATAAAAATTATCATCACATGGTATATCTGAAACACACGa < 1:3806718/67‑1 (MQ=255) cAGAAGCGACTGGAATACTAAGCACATCATAAAAATTATCATCACATGGTATATCTGAAACACACGa < 1:415985/67‑1 (MQ=255) aGAAGCGACTGGAGTACTAAGCACATCATAAAAATTATCATCACATGGTATATCTGAAACACACGAAAGCa < 1:3435548/71‑1 (MQ=255) aGAAGCGACTGGAGTACTAAGCACATCATAAAAATTATCATCACATGGTATATCTGAAACACACGAAAGCa < 1:3603101/71‑1 (MQ=255) aGAAGCGACTGGAATACTAAGCACATCATAAAAATTATCATCACATGGTATATCTGAAACACACGAAAGCa > 1:4097921/1‑71 (MQ=255) gAAGCGACTGGAATACTAAGCACATCATAAAAATTATCATCACATGGTATATCTGa < 1:3877102/56‑1 (MQ=255) gAAGCGACTGGAATACTAAGCACATCATAAAAATTATCATCACATGGTATATCTGa < 1:3343184/56‑1 (MQ=255) gAAGCGACTGGAATACTAAGCACATCATAAAAATTATCATCACATGGTATATCTGAAACACACGAAAGct > 1:1753123/1‑69 (MQ=255) gAAGCGACTGGAATACTAAGCACATCATAAAAATTATCATCACATGGTATATCTGAAACACACGAAAGCa > 1:1096040/1‑70 (MQ=255) gAAGCGACTGGAATACTAAGCACATCATAAAAATTATCATCACATGGTATATCTGAAACACACGAAAGCa > 1:701396/1‑70 (MQ=255) gAAGCGACTGGAATACTAAGCACATCATAAAAATTATCATCACATGGTATATCTGAAACACACGAAAGCa > 1:2650140/1‑70 (MQ=255) gAAGCGACTGGAATACTAAGCACATCATAAAAATTATCATCACATGGTATATCTGAAACACACGAAAGCAt < 1:3689813/71‑1 (MQ=255) gAAGCGACTGGAATACTAAGAACATCATAAAAATTATCATCACATGGTATATCTGa < 1:1975077/56‑1 (MQ=255) aaGCGACTGGAGTACTAAGCACATCATAAAAATTATCATCACATGGTATATCTGAAACACACGAAAGCa < 1:1954542/69‑1 (MQ=255) aaGCGACTGGAATACTAAGCACATCATAAAAATTATCATCACATGGTATATCTGa < 1:1235585/55‑1 (MQ=255) aGCGACTGGAATACTAAGCACATCATAAAAATTATCATCACATGGTatat > 1:2224499/1‑50 (MQ=255) aGCGACTGGAATACTAAGCACATCATAAAAATTATCATCACATGGTatat > 1:1394075/1‑50 (MQ=255) aGCGACTGGAATACTAAGCACATCATAAAAATTATCATCACATGGTatat > 1:2556686/1‑50 (MQ=255) aGCGACTGGAATACTAAGCACATCATAAAAATTATCATCACATGGTATATCTGaaa > 1:2790714/1‑56 (MQ=255) aGCGACTGGAATACTAAGCACATCATAAAAATTATCATCACATGGTATATCTGaaa > 1:3155539/1‑56 (MQ=255) aGCGACTGGAATACTAAGCACATCATAAAAATTATCATCACATGGTATATCTGAAACACACGAAAGCATAc > 1:3764308/1‑71 (MQ=255) gCGACTGGAATACTAAGCACATCATAAAAATTATCATCACATGGtat > 1:3458593/1‑47 (MQ=255) tGGAATACTAAGCACATCATAAAAATTATCATCACATGGTATATCTGAACCACACGAAAGCATACCTaaa > 1:2323171/1‑70 (MQ=255) tGGAATACTAAGCACATCATAAAAATTATCATCACATGGTATATCTGAAACACACGAAAGCATACCTAAAg > 1:1757028/1‑71 (MQ=255) tGGAATACTAAGCACATCATAAAAATTATCATCACATGGTATATCTGAAACACACGAAAGCATACCTAAAg > 1:3187121/1‑71 (MQ=255) tGGAATACTAAGCACATCATAAAAATTATCATCACATGGTATATCTGAAACACACGAAAGCATACCTAAAg > 1:891169/1‑71 (MQ=255) tGGAATACTAAGCACATCATAAAAATTATCATCACATGGTATATCTGAAACACACGAAAGCATACCTAAAg > 1:2869563/1‑71 (MQ=255) gAATACTAAGCACATCATAAAAATTATGTTCACATGTTATATCTGAAACACACGAAAGCATTCCTAAAg < 1:1014412/69‑1 (MQ=255) gAATACTAAGCACATCATAAAAATTATCATCACATTGTATATCTGAAACACACGAAAGCATACCTAAAGt < 1:2525824/70‑1 (MQ=255) gAATACTAAGCACATCATAAAAATTATCATCACATGGTATATCTGAAACACACGAAAGCATACCTAAAGt < 1:1671510/70‑1 (MQ=255) gAATACTAAGCACATCATAAAAATTATCATCACATGGTATATCTGAAACACACGAAAGCATACCTAAAGt < 1:3816326/70‑1 (MQ=255) gAATACTAAGCACATCATAAAAATTATCATCACATGGTATATCTGAAACACACGAAAGCATACCTAAAGt < 1:234620/70‑1 (MQ=255) gAATACTAAGCACATCATAAAAATTATCATCACATGGTATATCTGAAACACACGAAAGCATACCTAAAGt < 1:2475129/70‑1 (MQ=255) gAATACTAAGCACATCATAAAAATTATCATCACATGGTATATCTGAAACACACGAAAGCATACCTAAAGt > 1:3108988/1‑70 (MQ=255) gAATACTAAGCACATCATAAAAATTATCATCACATGGTATATCTGAAACACACGAAAGCATACCTAAAGt > 1:1122148/1‑70 (MQ=255) gAATACTAAGCACATCATAAAAATTATCATCACATGGTATATCTGAAACACACGAAAGCATACCTAAAGt < 1:2681067/70‑1 (MQ=255) aaTACTAAGCACATCATAAAAATTATCATCACATGGTATATCTGAAACACACGAAAGCATACCTAAAGTTa < 1:3169737/71‑1 (MQ=255) aaTACTAAGCACATCATAAAAATTATCATCACATGGTATATCTGAAACACACGAAAGCATACCTAAAGTTa < 1:2947569/71‑1 (MQ=255) aaTACTAAGCACATCATAAAAATTATCATCACATGGTATATCTGAAACACACGAAAGCATACCTAAAGTTa < 1:2895163/71‑1 (MQ=255) | GCCATCTATTTATAAATGTGTACAACGGGGATTATTTTTACATTGCATATTATTACCAGAAGCGACTGGAATACTAAGCACATCATAAAAATTATCATCACATGGTATATCTGAAACACACGAAAGCATACCTAAAGTTA > minE/2572488‑2572627 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |