BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,677,626	C→T	27.0%	A102T (GCA→ACA)	yhgA ←	predicted transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,677,626	0	C	T	27.0%	305.5 / 126.4	174	A102T (GCA→ACA)	yhgA	predicted transposase
Reads supporting (aligned to +/- strand): ref base C (50/76); new base T (20/27); total (71/103)
Fisher's exact test for biased strand distribution p-value = 7.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 176 total aligned reads displayed.

CCGTGGTAAAACAACAATGGCACCACCATCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGCGGCAATAGCGTAACGCATCATGCGAAATGCGATCAGTTTGTTTGAGGTGCTTTGATGTTCAATC  >  minE/2677556‑2677690

  gTGGTAAAACAACAATGGCACCACCATCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGTgg                                                                >  1:1620108/1‑71 (MQ=255)

  gTGGTAAAACAACAATGGCACCACCATCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGTgg                                                                >  1:2550496/1‑71 (MQ=255)

   tGGTAAAACAACAATGGCACCACCATCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGCGGc                                                               <  1:2533258/71‑1 (MQ=255)

     gTAAAACAACAATGGCACCACCATCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGTGGCa                                                              <  1:1461346/70‑1 (MQ=255)

      tAAAACAACAATGGCACCACCATCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGCGGCAAt                                                            <  1:2177297/71‑1 (MQ=255)

      tAAAACAACAATGGCACCACCATCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGCGGCAAt                                                            <  1:2208535/71‑1 (MQ=255)

        aaaCAACAATGGCACCACCATCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGCGGCaa                                                             <  1:2400599/68‑1 (MQ=255)

        aaaCAACAATGGCACCACCATCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGCGGCaa                                                             <  1:2679646/68‑1 (MQ=255)

        aaaCAACAATGGCACCACCATCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGCGGCAATAg                                                          >  1:459626/1‑71 (MQ=255)

           caacaaTGGCACCACCATCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGTGGCAATAGCGt                                                       <  1:3743855/71‑1 (MQ=255)

           caacaaTGGCACCACCATCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGTGGCAATAGCGt                                                       <  1:1761133/71‑1 (MQ=255)

           caacaaTGGCACCACCATCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGTGGCAATAGCGt                                                       <  1:3730314/71‑1 (MQ=255)

           caacaaTGGCACCACCATCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGTGGCAATAGCGt                                                       <  1:1085981/71‑1 (MQ=255)

           caacaaTGGCACCACCATCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGCGGCAATAGCGt                                                       <  1:3486160/71‑1 (MQ=255)

             acaaTGGCACCACCATCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGCGGCAATAGCGTaa                                                     >  1:897336/1‑71 (MQ=255)

             acaaTGGCACCACCATCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGCGGCAATAGCGTa                                                      >  1:793122/1‑70 (MQ=255)

                aTGGCACCACCATCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGCGGCAATAGCGTAACGc                                                  <  1:2477451/71‑1 (MQ=255)

                aTGGCACCACCATCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGCGGCAATAGCGTAACGc                                                  <  1:2994559/71‑1 (MQ=255)

                aTGGCACCACCATCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGCGGCAATAGCGTAACGc                                                  <  1:2027495/71‑1 (MQ=255)

                   gCACCACCATCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGTGGCAATAGCGTAACGcat                                                <  1:3251722/70‑1 (MQ=255)

                   gCACCACCATCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGTGGCAATAGCGTAACGcat                                                <  1:2341935/70‑1 (MQ=255)

                   gCACCACCATCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGCGGCAATAGCGTAACGcat                                                <  1:2358699/70‑1 (MQ=255)

                   gCACCACCATCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGCGGCAATAGCGTAACGcat                                                <  1:52436/70‑1 (MQ=255)

                   gCACCACCATCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGCGGCAATAGCGTAACGcat                                                <  1:2142771/70‑1 (MQ=255)

                   gCACCACCATCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGCGGCAATAGCGTAACGcat                                                <  1:702426/70‑1 (MQ=255)

                        tccaTCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATtgctgc                                                               <  1:3890696/49‑1 (MQ=255)

                        accaTCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGTGGCAATAGCGTAACGCATCATg                                            >  1:2665733/1‑69 (MQ=255)

                        accaTCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGTGGCAATAGCGTAACGCATCATg                                            >  1:2168052/1‑69 (MQ=255)

                         ccaTCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGCGGCAATAGCGTAACGCATCATGCGa                                         <  1:1248563/71‑1 (MQ=255)

                          caTCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGCGGCAATAGCGTAACGCATCATGCGaa                                        <  1:509083/71‑1 (MQ=255)

                           aTCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGCGGCAATAGCGTAACGcatca                                              >  1:4044130/1‑64 (MQ=255)

                           aTCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGCGGCAATAGCGTAACGCATCATGCGa                                         <  1:3100550/69‑1 (MQ=255)

                            tCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGCGGCAATAGCGTAACGCATCATGCGAAAt                                      >  1:3797124/1‑71 (MQ=255)

                            tCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGCGGCAATAGCGTAACGCATCATGCGAAAt                                      >  1:4113660/1‑71 (MQ=255)

                              ggCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGTGGCAATAGCGTAACGCATCATGCGAAATGc                                    >  1:1136314/1‑71 (MQ=255)

                              ggCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGTGGCAATAGCGTAACGCATCATGCGAAATGc                                    >  1:114425/1‑71 (MQ=255)

                                  aCGTTTTGTTTCCAGCATCAAGGTGATTTTGCATTGCGGCaa                                                             >  1:872843/1‑42 (MQ=255)

                                  aCGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGCGGCaa                                                             >  1:1886260/1‑42 (MQ=255)

                                  aCGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGCGGCaa                                                             >  1:1252069/1‑42 (MQ=255)

                                  aCGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGCGGCaa                                                             >  1:940770/1‑42 (MQ=255)

                                  aCGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGCGGCaa                                                             >  1:2518318/1‑42 (MQ=255)

                                  aCGTTTTGTATACAGCATCAAGGTGATTTTGCATTGCGGCaa                                                             >  1:720384/1‑42 (MQ=255)

                                     ttttGTATCCAGCATCAAGGTGATTTTGCATTGTGGc                                                               <  1:2041737/37‑1 (MQ=255)

                                     ttttGTATCCAGCATCAAGGTGATTTTGCATTGTGGc                                                               <  1:3495631/37‑1 (MQ=255)

                                     ttttGTATCCAGCATCAAGGTGATTTTGCATTGTGGc                                                               <  1:561966/37‑1 (MQ=255)

                                     ttttGTATCCAGCATCAAGGTGATTTTGCATTGTGGCAATAGCGt                                                       <  1:937482/45‑1 (MQ=255)

                                     ttttGTATCCAGCATCAAGGTGATTTTGCATTGTGGCAATAGCGt                                                       <  1:2229269/45‑1 (MQ=255)

                                     ttttGTATCCAGCATCAAGGTGATTTTGCATTGCGGCAATAGCGTAACGCATCATGCGAAATGCGATCAgt                             <  1:3510786/71‑1 (MQ=255)

                                     ttttGTATCCAGCATCAAGGTGATTTTGCATTGCGGCAATAGCGTAACGCATCATGCGAAATGCGATCAgt                             <  1:3661560/71‑1 (MQ=255)

                                     ttttGTATCCAGCATCAAGGTGATTTTGCATTGCGGCAATAGCGTAACGCATCATGCGAAATGCGATCAgt                             <  1:562248/71‑1 (MQ=255)

                                     ttttGTATCCAGCATCAAGGTGATTTTGCATTGCGGCAATAGCGTAACGCATCATGCGAAATGCGATCAgt                             <  1:3846388/71‑1 (MQ=255)

                                     ttttGTATCCAGCATCAAGGTGATTTTGCATTGCGGCAATAGCGTAACGCATCATGCGAAATGCGATCAgt                             <  1:805743/71‑1 (MQ=255)

                                      tttGTATCCAGCATCAAGGTGATTTTGCATTGTGGCAATAGCGTAACGCATCATGCGAAATGCGATCAgtt                            >  1:3093669/1‑71 (MQ=255)

                                      tttGTATCCAGCATCAAGGTGATTTTGCATTGTGGCAATAGCGTAACGCATCATGCGAAATGCGATCAgtt                            >  1:3944337/1‑71 (MQ=255)

                                       ttGTATCCAGCATCAAGGTGATTTTGCATTGCGGCAATAGCGTAACGCATCATGCGAAATGCGATCAgttt                           >  1:2615665/1‑71 (MQ=255)

                                       ttGTATCCAGCATCAAGGTGATTTTGCATTGCGGCAATAGCGTAACGCATCATGCGAAATGCGATCAgttt                           >  1:1489387/1‑71 (MQ=255)

                                         gTATCCAGCATCAAGGTGATTTTGCATTGCGGCAATAGCGTAACGCATCATg                                            >  1:3306705/1‑52 (MQ=255)

                                         gTATCCAGCATCAAGGTGATTTTGCATTGCGGCAATAGCGTAACGCATCATGCGAAATGCGATCAgtttgt                         >  1:2458217/1‑71 (MQ=255)

                                         gTATCCAGCATCAAGGTGATTTTGCATTGCGGCAATAGCGTAACGCATCATGCGAAATGCGATCAgtttg                          <  1:486209/70‑1 (MQ=255)

                                         gTATCCAGCATCAAGGTGATTTTGCATTGCGGCAATAGCGTAACGCATCATGCGAAATGCGATCAgtttg                          <  1:3991562/70‑1 (MQ=255)

                                         gTATCCAGCATCAAGGTGATTTTGCATTGCGGCAATAGCGTAACGCATCATGCGAAATGCGATCAgtttg                          <  1:115726/70‑1 (MQ=255)

                                            tCCAGCATCAAGGTGATTTTGCATTGCGGCAATAGCGTAACGc                                                  <  1:1996564/43‑1 (MQ=255)

                                            tCCAGCATCAAGGTGATTTTGCATTGCGGCAATAGCGTAACGc                                                  <  1:3661031/43‑1 (MQ=255)

                                            tCCAGCATCAAGGTGATTTTGCATTGCGGCAATAGCGTAACGc                                                  <  1:3115439/43‑1 (MQ=255)

                                            tCCAGCATCAAGGTGATTTTGCATTGCGGCAATAGCGTAACGCATCATGCGAAATGCGATCAGtttgtttg                      <  1:1264382/71‑1 (MQ=255)

                                            tCCAGCATCAAGGTGATTTTGCATTGCGGCAATAGCGTAACGCATCATGCGAAATGCGATCAGtttgtttg                      <  1:3550619/71‑1 (MQ=255)

                                            tCCAGCATCAAGGTGATTTTGCATTGCGGCAATAGCGTAACGCATCATGCGAAATGCGATCAGtttgtttg                      <  1:1588567/71‑1 (MQ=255)

                                              cAGCATCAAGGTGATTTTGCATTGCGGCAATAGCGTAACGCATCATGCGAAATGCGATCAGTTTGTTTGAg                    >  1:1000146/1‑71 (MQ=255)

                                                  aTCAAGGTGATTTTGCATTGCGGCAATAGCGTAACGCATCATGCGAAATGCGATCAGTTTGTTTGAGGTGc                >  1:1386652/1‑71 (MQ=255)

                                                  aTCAAGGTGATTTTGCATTGCGGCAATAGCGTAACGCATCATGCGAAATGCGATCAGTTTGTTTGAGGTGc                >  1:224569/1‑71 (MQ=255)

                                                  aTCAAGGTGATTTTGCATTGCGGCAATAGCGTAACGCATCATGCGAAATGCGATCAGTTTGTTTGAGGTGc                >  1:3855288/1‑71 (MQ=255)

                                                   tCAAGGTGATTTTGCATTGCGGCAATAGCGTAACGCATCATGCGAAATGCGATCAGTTTGTTTGAg                    >  1:3038934/1‑66 (MQ=255)

                                                     aaGGTGATTTTGCATTGCGGCAATAGCGTAAGGCATCATGCGAAATGCGATCAGTTTGTTTGAGGTGCttt             <  1:2817590/71‑1 (MQ=255)

                                                     aaGGTGATTTTGCATTGCGGCAATAGCGTAACGCATCATGCGAAATGCGATCAGTTTGTTTGAGGTGCttt             <  1:3202547/71‑1 (MQ=255)

                                                     aaGGTGATTTTGCATTGCGGCAATAGCGTAACGCATCATGCGAAATGCGATCAGTTTGTTTGAGGTGCttt             <  1:2591318/71‑1 (MQ=255)

                                                      aGGTGATTTTGCATTGTGGCAATAGCGTAACGCATCATGCGAAATGCGATCAGTTTGTTTGAGGTGCTTTg            <  1:3615682/71‑1 (MQ=255)

                                                      aGGTGATTTTGCATTGCGGCAATAGCGTAACGCATCATGCGAAATGCGATCAgtttgttt                       <  1:1174991/60‑1 (MQ=255)

                                                      aGGTGATTTTGCATTGCGGCAATAGCGTAACGCATCATGCGAAATGCGATCAGTTTGTTTGAGGTGCTTTg            <  1:2730314/71‑1 (MQ=255)

                                                      aGGTGATTTTGCATTGCGGCAATAGCGTAACGCATCATGCGAAATGCGATCAGTTTGTTTGAGGTGCTTTg            <  1:1011505/71‑1 (MQ=255)

                                                      aGGTGATTTTGCATTGCGGCAATAGCGTAACGCATCATGCGAAATGCGATCAGTTTGTTTGAGGTGCTTTg            <  1:3138046/71‑1 (MQ=255)

                                                        gTGATTTTGCATTGCGGCAATAGCGTAACGCATCATGCGAAATGCGATCAGTTTGTTTGa                     >  1:622679/1‑60 (MQ=255)

                                                        gTGATTTTGCATTGCGGCAATAGCGTAACGCATCATGCGAAATGCGATCAGTTTGTTTGa                     >  1:2257150/1‑60 (MQ=255)

                                                        gTGATTTTGCATTGCGGCAATAGCGTAACGCATCATGCGAAATGCGATCAGTTTGTTTGa                     >  1:2480581/1‑60 (MQ=255)

                                                           aTTTTGCATTGTGGCAATAGCGTAACGCATCATGCGAAATGCGATCAGTTTGTTTGAGGTGCTTTGATGtt       <  1:2926743/71‑1 (MQ=255)

                                                           aTTTTGCATTGTGGCAATAGCGTAACGCATCATGCGAAATGCGATCAGTTTGTTTGAGGTGCTTTGATGtt       <  1:202103/71‑1 (MQ=255)

                                                           aTTTTGCATTGCGGCAATAGCGTAACGCATCATGCGAAATGCGATCAGTTTGTTTGAGGTGCTTTGATGtt       >  1:3299268/1‑71 (MQ=255)

                                                            ttttGCATTGTGGCAATAGCGTAACGCATCATGCGAAATGCGATCAgtttg                          <  1:4093469/51‑1 (MQ=255)

                                                             tttGCATTGTGGCAATAGCGTAACGCATCATGCGAAATGCGATCAGTTTGTTTGAGGTGCTTTGATGTTc      >  1:2853785/1‑70 (MQ=255)

                                                             tttGCATTGTGGCAATAGCGTAACGCATCATGCGAAATGCGATCAGTTTGTTTGAGGTGCTTTGATGTTc      >  1:142214/1‑70 (MQ=255)

                                                              ttGCATTGTGGCAATAGCGTAACGCATCATGCGAAATGCGATCAGTTTGTTTGAGGTGCTTTGATGTTCaa    >  1:658378/1‑71 (MQ=255)

                                                              ttGCATTGCGGCAATAGCGTAACGCATCATGCGAAATGCGATCAGTTTGTTTGAgg                   >  1:1939228/1‑56 (MQ=255)

                                                              ttGCATTGCGGCAATAGCGTAACGCATCATGCGAAATGCGATCAGTTTGTTTGAgg                   >  1:1758652/1‑56 (MQ=255)

                                                              ttGCATTGCGGCAATAGCGTAACGCATCATGCGAAATGCGATCAGTTTGTTTGAGGTGCTTTGATGTTCaa    >  1:1868978/1‑71 (MQ=255)

                                                                 cATTGTGGCAATAGCGTAACGCATCATGCGAAATGCGATCAGTTTGTTTGAGGTGCTTTGATGTTCAATc  <  1:1809109/70‑1 (MQ=255)

                                                                   ttGCGGCAATAGCGTAACGCATCATGCGAAATGCGATCAGTTTGTTTGAGGTGCTTTGATGTTCAAt   <  1:2359631/67‑1 (MQ=255)

                                                                   ttGCGGCAATAGCGTAACGCATCATGCGAAATGCGATCAGTTTGTTTGAGGTGCTTTGATGTTCAAt   <  1:2316300/67‑1 (MQ=255)

                                                                   ttGCGGCAATAGCGTAACGCATCATGCGAAATGCGATCAGTTTGTTTGAGGTGCTTTGATGTTCAAt   <  1:1830773/67‑1 (MQ=255)

                                                                    tGCGGCAATAGCGTAACGCATCATGCGAAATGCGATCAGTTTGTTTGAgg                   >  1:2082538/1‑50 (MQ=255)

                                                                    tGCGGCAATAGCGTAACGCATCATGCGAAATGCGATCAGTTTGTTTGAgg                   >  1:3367625/1‑50 (MQ=255)

                                                                    tGCGGCAATAGCGTAACGCATCATGCGAAATGCGATCAGTTTGTTTGAgg                   >  1:769702/1‑50 (MQ=255)

CCGTGGTAAAACAACAATGGCACCACCATCGGCAACGTTTTGTATCCAGCATCAAGGTGATTTTGCATTGCGGCAATAGCGTAACGCATCATGCGAAATGCGATCAGTTTGTTTGAGGTGCTTTGATGTTCAATC  >  minE/2677556‑2677690

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`