| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,687,159 | G→A | 26.2% | L374L (CTG→TTG) | pck ← | phosphoenolpyruvate carboxykinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,687,159 | 0 | G | A | 26.2% | 152.3 / 57.1 | 84 | L374L (CTG→TTG) | pck | phosphoenolpyruvate carboxykinase |
| Reads supporting (aligned to +/- strand): ref base G (29/33); new base A (12/10); total (41/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.23e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
CTCAGTACCGGCCAGTTTGGCGGTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAGGAAGATAACCTTAGTCGCGTGGCCCGC > minE/2687094‑2687228 | cTCAGTACCGGCCAGTTTGGCGGTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAgg > 1:36607/1‑67 (MQ=255)cTCAGTACCGGCCAGTTTGGCGGTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGc > 1:365804/1‑68 (MQ=255)cTCAGTACCGGCCAGTTTGGCGGTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGc > 1:3306874/1‑68 (MQ=255)cTCAGTACCGGCCAGTTTGGCGGTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGc > 1:2713568/1‑68 (MQ=255)cTCAGTACCGGCCAGTTTGGCGGTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGc > 1:232029/1‑68 (MQ=255)cTCAGTACCGGCCAGTTTGGCGGTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGc > 1:1385561/1‑68 (MQ=255) aGTACCGGCCAGTTTGGCGGTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGaa < 1:2293445/70‑1 (MQ=255) aGTACCGGCCAGTTTGGCGGTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGaa < 1:2288659/70‑1 (MQ=255) tACCGGCCAGTTTGGCGGTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAAcc < 1:549053/71‑1 (MQ=255) tACCGGCCAGTTTGGCGGTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAAcc < 1:185585/71‑1 (MQ=255) aCCGGCCAGTTTGGCGGTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACcg > 1:3498336/1‑71 (MQ=255) aCCGGCCAGTTTGGCGGTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACcg > 1:3673676/1‑71 (MQ=255) aGTTTGGCGGTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCaa < 1:124997/71‑1 (MQ=255) aGTTTGGCGGTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCaa < 1:2987925/71‑1 (MQ=255) aGTTTGGCGGTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCaa < 1:3052028/71‑1 (MQ=255) gTTTGGCGGTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAac < 1:116867/71‑1 (MQ=255) gTTTGGCGGTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAac < 1:2154011/71‑1 (MQ=255) gTTTGGCGGTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAac < 1:3083181/71‑1 (MQ=255) ttGGCGGTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAacac > 1:2625619/1‑71 (MQ=255) tGGCGGTGAAGCGAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAAGCGAGAAACCGGCGGCAACACg > 1:386554/1‑71 (MQ=255) tGGCGGTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAAGCGAGAAACCGGCGGCAACACg > 1:3084067/1‑71 (MQ=255) tGGCGGTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAAGCGAGAAACCGGCGGCAACACg > 1:1598359/1‑71 (MQ=255) tGGCGGTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAAGCGAGAAACCGGCGGCAACACg > 1:3048923/1‑71 (MQ=255) gCGGTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAac < 1:2241583/66‑1 (MQ=255) ggTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGa < 1:3964244/71‑1 (MQ=255) ggTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGa < 1:1324371/71‑1 (MQ=255) gTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGa < 1:1556651/70‑1 (MQ=255) gTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAAGCGAGAAACCGGCGGCAACACGCCGaa < 1:3898577/71‑1 (MQ=255) gTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAAGCGAGAAACCGGCGGCAACACGCCGaa < 1:2556903/71‑1 (MQ=255) gAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAg > 1:1539793/1‑71 (MQ=255) aaGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACcgg > 1:2713765/1‑53 (MQ=255) aaGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACcgg > 1:1088133/1‑53 (MQ=255) aaGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACcgg > 1:2313494/1‑53 (MQ=255) aaGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACcgg > 1:1739259/1‑53 (MQ=255) aaGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACcgg > 1:1926969/1‑53 (MQ=255) aaGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAAGCGAGAAAcc < 1:93029/51‑1 (MQ=255) aaGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAAGCGAGAAAcc < 1:204673/51‑1 (MQ=255) aaGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAAGCGAGAAAcc < 1:4113646/51‑1 (MQ=255) aaGCCAGAGAGGAAGTGATACTCGGTTTGATCGGCAGTCAAGCGAGAAAcc < 1:1866825/51‑1 (MQ=255) aGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAGCa > 1:2746050/1‑71 (MQ=255) aGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAGCa > 1:3998399/1‑71 (MQ=255) gCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAGCa > 1:964634/1‑70 (MQ=255) gCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAAGCGAGaaa > 1:2822131/1‑47 (MQ=255) gagagGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAGCATcag > 1:2505281/1‑70 (MQ=255) gagagGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAGCATcag > 1:433317/1‑70 (MQ=255) gagGAAGTGATACTGGGTTTGATCGGCAGTCAGGCgaga < 1:1706823/39‑1 (MQ=255) gagGAAGTGATACTGGGTTTGATCGGCAGTCAGGCgaga < 1:2311391/39‑1 (MQ=255) gagGAAGTGATACTGGGTTTGATCGGCAGTCAGGCgaga < 1:1323970/39‑1 (MQ=255) gagGAAGTGATACTGGGTTTGATCGGCAGTCAGGCgaga < 1:1752432/39‑1 (MQ=255) gagGAAGTGATACTGGGTTTGATCGGCAGTCAGGCcaga < 1:3085877/39‑1 (MQ=255) aGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAGCATcagc < 1:2054197/64‑1 (MQ=255) aGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAGCATcagc < 1:2285906/64‑1 (MQ=255) aGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAGCATcagc < 1:2276499/64‑1 (MQ=255) aGTGATACTGGGTTTGATCGGCAGTCAAGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAg > 1:384883/1‑70 (MQ=255) gTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAac < 1:2097657/46‑1 (MQ=255) gTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAac < 1:2409918/46‑1 (MQ=255) gTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAac < 1:3190513/46‑1 (MQ=255) tGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAgg > 1:3962615/1‑69 (MQ=255) tGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAgg > 1:299025/1‑69 (MQ=255) tGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAGGaa < 1:1311052/71‑1 (MQ=255) gATACTGGGTTTGATCGGCAGTCAAGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAgg > 1:123239/1‑68 (MQ=255) aTACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAGGAAGa < 1:233922/71‑1 (MQ=255) cTGGGTTTGATCGGCAGTCAAGCGAGAAACCGGCGGCAaca > 1:2929947/1‑41 (MQ=255) cTGGGTTTGATCGGCAGTCAAGCGAGAAACCGGCGGCAaca > 1:2628215/1‑41 (MQ=255) cTGGGTTTGATCGGCAGTCAAGCGAGAAACCGGCGGCAaca > 1:1296084/1‑41 (MQ=255) tGGGTTTGATCGGCAGTCAAGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAGGAAGATAAc > 1:576758/1‑71 (MQ=255) gggTTTGATCGGCAGTCAAGCGAGAAACCGGAGGCAACACGCCGAAAGCATAAGCAGTCAGGAAGATAAcc > 1:2299513/1‑71 (MQ=255) ggTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAGGAAGATAAcc < 1:775589/70‑1 (MQ=255) ggTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAGGAAGATAACCt < 1:2502153/71‑1 (MQ=255) gTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCTACACGCCGAAAGCATCAGCAGTCAGGAAGATAACCtt < 1:3221188/71‑1 (MQ=255) gTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAGGAAGATAAcc < 1:2051769/69‑1 (MQ=255) gTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAGGAAGATAAcc < 1:191082/69‑1 (MQ=255) tttGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAGGAAGATAACCt > 1:798649/1‑69 (MQ=255) tttGATCGGCAGTCAAGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAGGAAGATAACCTTa < 1:1546122/71‑1 (MQ=255) ttGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAGGAAGATAACCtt > 1:2857125/1‑69 (MQ=255) tGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAGGAAGATAACCTTAGt > 1:2947200/1‑71 (MQ=255) gCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAGGAAGa < 1:965060/55‑1 (MQ=255) tCAAGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAGGaa < 1:2501467/49‑1 (MQ=255) tCAAGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAGGaa < 1:2100417/49‑1 (MQ=255) tCAAGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAGGaa < 1:1179141/49‑1 (MQ=255) aGGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAGGAATATAACCTTAGTCGCGTGGCCCGc > 1:3276145/1‑71 (MQ=255) aGGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAGGAAGATAACCTTAGTCGCGTGGCCCg > 1:1060305/1‑70 (MQ=255) aGGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAGGAAGATAACCTTAGTCGCGTGGCCCGc > 1:3340338/1‑71 (MQ=255) aGGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAGGAAGATAACCTTAGTCGCGTGGCCCGc > 1:1904152/1‑71 (MQ=255) aGGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAGGAAGATAACCTTAGTCGCGTGGCCCGc > 1:1259279/1‑71 (MQ=255) | CTCAGTACCGGCCAGTTTGGCGGTGAAGCCAGAGAGGAAGTGATACTGGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAGCATCAGCAGTCAGGAAGATAACCTTAGTCGCGTGGCCCGC > minE/2687094‑2687228 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |