| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,732,642 | C→A | 25.2% | Q238K (CAA→AAA) | yheO → | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,732,642 | 0 | C | A | 25.2% | 309.3 / 66.8 | 123 | Q238K (CAA→AAA) | yheO | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (49/43); new base A (6/25); total (55/68) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.48e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.65e-01 | |||||||||||
Only 100 of 123 total aligned reads displayed.
CATCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCAACAGGCGAGTA > minE/2732581‑2732707 | cATCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGt < 1:2981059/71‑1 (MQ=255)cATCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGt < 1:3311196/71‑1 (MQ=255)cATCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGt < 1:2032671/71‑1 (MQ=255)cATCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGt < 1:3649291/71‑1 (MQ=255) aTCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAg > 1:565243/1‑69 (MQ=255) aTCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGt > 1:15885/1‑70 (MQ=255) aTCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGt > 1:737119/1‑70 (MQ=255) ccAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGAAAGATAAGTAATGc > 1:1622950/1‑71 (MQ=255) cAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCg > 1:1032502/1‑71 (MQ=255) cAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCg > 1:1575593/1‑71 (MQ=255) aaCACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGtt < 1:4013334/71‑1 (MQ=255) acacacTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGttt < 1:3374529/71‑1 (MQ=255) acacacTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGttt < 1:269994/71‑1 (MQ=255) cacacTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAg < 1:246867/55‑1 (MQ=255) cacacTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAg < 1:2134761/55‑1 (MQ=255) cacacTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAg < 1:3665366/55‑1 (MQ=255) cacacTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAg < 1:2673847/55‑1 (MQ=255) cacacTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAg < 1:3018267/55‑1 (MQ=255) cacacTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAg < 1:1029325/55‑1 (MQ=255) cacacTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAg < 1:1090735/55‑1 (MQ=255) cacacTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAg < 1:133019/55‑1 (MQ=255) cacacTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGt < 1:2937503/61‑1 (MQ=255) acacTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGt < 1:490406/60‑1 (MQ=255) cacTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTaa > 1:2569979/1‑61 (MQ=255) acTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGt < 1:2424768/58‑1 (MQ=255) acTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGAAAGATAAGTAATGCGTTTTGcc < 1:871681/71‑1 (MQ=255) tCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCg > 1:1539235/1‑71 (MQ=255) tCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCg > 1:3462550/1‑71 (MQ=255) cTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTaa > 1:2685286/1‑55 (MQ=255) cTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTaa > 1:3554560/1‑55 (MQ=255) cTATCTCTACATACGCCAGTTCAAGAGAGGTGATTTCAAGGGGCAAg > 1:131325/1‑47 (MQ=255) tctcTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCa < 1:2647344/42‑1 (MQ=255) tctcTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCa < 1:331444/42‑1 (MQ=255) tctcTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCa < 1:1492347/42‑1 (MQ=255) tctcTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCgtggt > 1:2337703/1‑71 (MQ=255) tctcTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCgtggt > 1:634627/1‑71 (MQ=255) tctcTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCgt < 1:1386101/68‑1 (MQ=255) tctcTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCgt < 1:925224/68‑1 (MQ=255) tctcTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCgt < 1:2513328/68‑1 (MQ=255) tctcTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCgt < 1:3661756/68‑1 (MQ=255) tctACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGa > 1:3786448/1‑71 (MQ=255) tctACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGa > 1:3771394/1‑71 (MQ=255) tctACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGa > 1:1883879/1‑71 (MQ=255) tACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCgt < 1:573842/64‑1 (MQ=255) tACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCgt < 1:578603/64‑1 (MQ=255) tACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCgt < 1:4019528/64‑1 (MQ=255) tACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGAcc > 1:1751125/1‑71 (MQ=255) cATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGc > 1:82993/1‑56 (MQ=255) cATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGc > 1:2894421/1‑56 (MQ=255) cATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGc > 1:1476020/1‑56 (MQ=255) cATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCgg > 1:234318/1‑71 (MQ=255) cATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCgg > 1:3200817/1‑71 (MQ=255) cATCCGCCAGTTAAAGAGCGGTGATTTCCAGGGGCAAg > 1:317090/1‑38 (MQ=255) ccGCAAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATATGTAATGCTTTTTGCCATCgt < 1:2027913/59‑1 (MQ=255) cGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATaa > 1:3958651/1‑38 (MQ=255) cGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATaa > 1:4076715/1‑38 (MQ=255) cGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATaa > 1:150529/1‑38 (MQ=255) cGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATaa > 1:332245/1‑38 (MQ=255) cGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATaa > 1:3275630/1‑38 (MQ=255) gCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAg < 1:3190136/71‑1 (MQ=255) cAGTTCAAGAGCGGTGATTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCa < 1:2121230/71‑1 (MQ=255) cAGTTCAAGAGCGGTGATTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCa < 1:958249/71‑1 (MQ=255) gTTCAAGAGCGGTGATTTACAGGGGAAAGATAAGTAATGCGTTTTGCCATCgt < 1:1263685/53‑1 (MQ=255) ttCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCgg < 1:3025540/61‑1 (MQ=255) cAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGcc > 1:3148239/1‑45 (MQ=255) cAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACgg > 1:2787227/1‑71 (MQ=255) cAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACgg > 1:2256889/1‑71 (MQ=255) cAAGAGCGGTGATTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACgg > 1:2772987/1‑71 (MQ=255) cAAGAGCGGTGATTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACgg > 1:3913832/1‑71 (MQ=255) aaGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACgg < 1:2578238/70‑1 (MQ=255) aaGAGCGGTGATTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGt < 1:683657/71‑1 (MQ=255) aaGAGCGGTGATTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGt < 1:960870/71‑1 (MQ=255) aaGAGCGGTGATTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGt < 1:965631/71‑1 (MQ=255) aaGAGCGGTGATTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGt < 1:2233162/71‑1 (MQ=255) aaGAGCGGTGATTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGt < 1:2541079/71‑1 (MQ=255) aaGAGCGGTGATTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGt < 1:344930/71‑1 (MQ=255) agagCGGTGATTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGt < 1:2865868/70‑1 (MQ=255) cGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCa < 1:2397275/71‑1 (MQ=255) cGGTGATTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCa < 1:1692960/71‑1 (MQ=255) ggTGATTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCa < 1:13637/70‑1 (MQ=255) gTGATTTCCAGGGGCAAGATAAGTATTGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCaa > 1:516603/1‑70 (MQ=255) gTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCaa > 1:1617698/1‑70 (MQ=255) gTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCaa > 1:1168214/1‑70 (MQ=255) gTGATTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCAAc < 1:3730519/71‑1 (MQ=255) gTGATTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCAAc < 1:4082849/71‑1 (MQ=255) tGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATa < 1:490011/59‑1 (MQ=255) gATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATc > 1:3456275/1‑38 (MQ=255) gATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCAACAg > 1:2852991/1‑71 (MQ=255) aTTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACgg < 1:835565/60‑1 (MQ=255) aTTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCAACAgg > 1:2733862/1‑71 (MQ=255) aTTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCAACAg > 1:3094202/1‑70 (MQ=255) aTTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCACCATACgg < 1:113564/60‑1 (MQ=255) ttCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTa > 1:3962326/1‑60 (MQ=255) ttCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTa > 1:2781763/1‑60 (MQ=255) tCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCAACAGGc > 1:1742456/1‑69 (MQ=255) tCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCAACAGGc > 1:2904462/1‑69 (MQ=255) cAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCAACAGGCGag < 1:881378/70‑1 (MQ=255) aGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATa < 1:1768020/51‑1 (MQ=255) aGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCAACAGGCGagta < 1:1928465/71‑1 (MQ=255) aGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCAACAGGCGagta < 1:1849283/71‑1 (MQ=255) | CATCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCAACAGGCGAGTA > minE/2732581‑2732707 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |