| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,850,177 | G→A | 36.8% | Q286* (CAG→TAG) | yjeP ← | predicted mechanosensitive channel |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,850,177 | 0 | G | A | 36.8% | 51.3 / 58.7 | 57 | Q286* (CAG→TAG) | yjeP | predicted mechanosensitive channel |
| Reads supporting (aligned to +/- strand): ref base G (21/15); new base A (11/10); total (32/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.84e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.32e-01 | |||||||||||
TGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATT > minE/2850110‑2850244 | tGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTg > 1:3621087/1‑71 (MQ=255) gCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGc > 1:1075304/1‑71 (MQ=255) gCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGc > 1:490937/1‑71 (MQ=255) gCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGc > 1:1835419/1‑71 (MQ=255) gCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGc > 1:259373/1‑71 (MQ=255) gCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGc > 1:2488568/1‑71 (MQ=255) gTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTATTGCGAGGc > 1:560946/1‑70 (MQ=255) gTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTATTGCGAGGc > 1:190428/1‑70 (MQ=255) gTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTATTGCGAGGc > 1:3868450/1‑70 (MQ=255) ttCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGc < 1:1940961/69‑1 (MQ=255) ttCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCaa > 1:564117/1‑71 (MQ=255) ttCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTATTGCGAGGc < 1:1335028/69‑1 (MQ=255) ttCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTATTGCGAGGc < 1:549357/69‑1 (MQ=255) ttCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTATTGCGAGGc < 1:3584436/69‑1 (MQ=255) cACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAAc > 1:1581767/1‑70 (MQ=255) aCGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACg < 1:993786/70‑1 (MQ=255) aCGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTATTGCGAGGCAACga < 1:788153/71‑1 (MQ=255) aCGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTATTGCGAGGCAACga < 1:2577874/71‑1 (MQ=255) aCGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTATTGCGAGGCAACga < 1:3815338/71‑1 (MQ=255) aCGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTATTGCGAGGCAACga < 1:1691244/71‑1 (MQ=255) cAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTATTGCGAGGCAACGAGat < 1:436966/71‑1 (MQ=255) aGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTATTGCGAGGCAACGAGat < 1:150940/70‑1 (MQ=255) gCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGatcc < 1:744112/71‑1 (MQ=255) tATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGatc < 1:3831861/67‑1 (MQ=255) ttCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCg > 1:525007/1‑71 (MQ=255) cAACGCGTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCt > 1:530837/1‑71 (MQ=255) cAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGc > 1:2775253/1‑70 (MQ=255) cAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCt > 1:1812018/1‑71 (MQ=255) cAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCt > 1:3595580/1‑71 (MQ=255) cAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCt > 1:2981497/1‑71 (MQ=255) cAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCt > 1:2725512/1‑71 (MQ=255) cAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTATTGCGAGGCAACGAGATCCATCCGCt > 1:3671733/1‑71 (MQ=255) aCGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTgc < 1:625222/71‑1 (MQ=255) aCGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTgc < 1:2611159/71‑1 (MQ=255) aCGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTgc < 1:2165789/71‑1 (MQ=255) ggACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGa < 1:947085/71‑1 (MQ=255) aCCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGatc < 1:3205760/50‑1 (MQ=255) gTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCa > 1:1715366/1‑69 (MQ=255) gTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCa > 1:1983068/1‑69 (MQ=255) aCGTCTGGCTGGCAGCCTGACGCTATTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAgcc > 1:1001070/1‑71 (MQ=255) aCGTCTGGCTGGCAGCCTGACGCTATTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAgcc > 1:3857520/1‑71 (MQ=255) aCGTCTGGCTGGCAGCCTGACGCTATTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAgcc > 1:2411858/1‑71 (MQ=255) gTCTGGCTGGCAGCCTGACGCTATTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGAt > 1:2311450/1‑61 (MQ=255) tCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAgccgcc < 1:394436/71‑1 (MQ=255) tCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAgccgcc < 1:2399883/71‑1 (MQ=255) tCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAgccgcc < 1:8452/71‑1 (MQ=255) tCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAgccgcc < 1:814750/71‑1 (MQ=255) tCTGGCTGGCAGCCTGACGCTATTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAgccgcc < 1:3750766/71‑1 (MQ=255) tggctggcAGCCTGACGCTATTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAgccgc > 1:201801/1‑68 (MQ=255) gctggcAGCCTGACGCTGTTGCGAGGCAAGGAGATCCATCCGCTGCGCCTGTTGATTCAAAGccgccg < 1:417307/68‑1 (MQ=255) gctggcAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGccgccg < 1:3913805/68‑1 (MQ=255) ctggcAGCCTGACGCTGTTGCGAGGCAACGAGatccat > 1:2153457/1‑38 (MQ=255) gcAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGa > 1:2019684/1‑65 (MQ=255) gcAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGa > 1:3386023/1‑65 (MQ=255) gcAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGa > 1:2638596/1‑65 (MQ=255) gCTATTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAAtt > 1:3648183/1‑71 (MQ=255) gCTATTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAAtt > 1:3799361/1‑71 (MQ=255) | TGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATT > minE/2850110‑2850244 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |