| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 297,667 | C→T | 15.8% | R110C (CGT→TGT) | mdlA → | fused predicted multidrug transporter subunits and ATP‑binding components of ABC superfamily |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 297,667 | 0 | C | T | 15.8% | 144.6 / 17.9 | 57 | R110C (CGT→TGT) | mdlA | fused predicted multidrug transporter subunits and ATP‑binding components of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base C (19/29); new base T (6/3); total (25/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.61e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01 | |||||||||||
AACTGGCTGTTGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCC > minE/297599‑297732 | aaCTGGCTGTTGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGt < 1:560159/71‑1 (MQ=255) aCTGGCTGTTGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGTGTc < 1:2196591/71‑1 (MQ=255) aCTGGCTGTTGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTc < 1:486918/71‑1 (MQ=255) aCTGGCTGTTGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTc < 1:3329687/71‑1 (MQ=255) cTGGCTGTTGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCa < 1:1325912/71‑1 (MQ=255) cTGGCTGTTGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCa < 1:3862720/71‑1 (MQ=255) cTGGCTGTTGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCa < 1:146100/71‑1 (MQ=255) cTGGCTGTTGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCa < 1:2584838/71‑1 (MQ=255) cTGGCTGTTGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCa < 1:1957394/71‑1 (MQ=255) gCTGTTGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCAt < 1:2527946/69‑1 (MQ=255) cTGTTGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTc < 1:1013974/66‑1 (MQ=255) cTGTTGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGc < 1:2530256/71‑1 (MQ=255) gTTGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCAc > 1:1094273/1‑71 (MQ=255) gTTGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCAc > 1:901631/1‑71 (MQ=255) gTTGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCAc > 1:2286773/1‑71 (MQ=255) gTTGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCAc > 1:193534/1‑71 (MQ=255) ttGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCAcc < 1:1926936/71‑1 (MQ=255) ttGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCAcc < 1:3070388/71‑1 (MQ=255) ttGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCAcc < 1:691202/71‑1 (MQ=255) ttGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCAcc < 1:2844772/71‑1 (MQ=255) tGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGTGTCATCGCACCg > 1:3845195/1‑71 (MQ=255) tGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGTGTCATCGCACCg > 1:3519494/1‑71 (MQ=255) tGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGTGTCATCGCACCg > 1:2532622/1‑71 (MQ=255) gAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCgg > 1:3473139/1‑71 (MQ=255) aaCTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGt < 1:331015/71‑1 (MQ=255) aaCTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGt < 1:551212/71‑1 (MQ=255) aaCTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGt < 1:2905320/71‑1 (MQ=255) aaCTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGt < 1:1516770/71‑1 (MQ=255) aCTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGt < 1:2777866/70‑1 (MQ=255) cTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGTGTCATCGCACCGGTg < 1:3062186/70‑1 (MQ=255) aaGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATg > 1:3695406/1‑71 (MQ=255) aGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAAt < 1:850028/71‑1 (MQ=255) aGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAAt < 1:1951483/71‑1 (MQ=255) cTAAGCCGGCAGCATCCTGAGTTTTACCTGTGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGa < 1:3539429/71‑1 (MQ=255) gCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAAt < 1:3633436/65‑1 (MQ=255) gcagcaTCCTGAGTTTTACCTGTGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGAt > 1:1533423/1‑70 (MQ=255) gcagcaTCCTGAGTTTTACCTGTGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATc > 1:1473785/1‑71 (MQ=255) atacTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGcgtc > 1:1677628/4‑71 (MQ=255) aTCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCg > 1:2821918/1‑46 (MQ=255) aTCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCg > 1:2213107/1‑46 (MQ=255) aTCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCg > 1:1464008/1‑46 (MQ=255) aTCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGcgtc > 1:2358287/1‑71 (MQ=255) aTCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGcgtc > 1:1842132/1‑71 (MQ=255) tCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGAc < 1:1447252/51‑1 (MQ=255) tCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGAc < 1:1193009/51‑1 (MQ=255) tCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGAc < 1:227928/51‑1 (MQ=255) cTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATg > 1:1344157/1‑38 (MQ=255) cTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGAt > 1:3699166/1‑62 (MQ=255) cTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGAt > 1:539088/1‑62 (MQ=255) cTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGAt > 1:1201554/1‑62 (MQ=255) cTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGAt > 1:670170/1‑62 (MQ=255) cTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCgtg > 1:700888/1‑71 (MQ=255) cTGAGTTTTACATGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGAt > 1:1637804/1‑62 (MQ=255) ttttACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGAcc < 1:3563669/45‑1 (MQ=255) ttttACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGAcc < 1:3256329/45‑1 (MQ=255) ttttACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGAcc < 1:2512436/45‑1 (MQ=255) aCCTGTGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTgccgcc > 1:2017491/1‑71 (MQ=255) | AACTGGCTGTTGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCC > minE/297599‑297732 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |