| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,864,584 | C→T | 14.0% | A295A (GCC→GCT) | hflK → | modulator for HflB protease specific for phage lambda cII repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,864,584 | 0 | C | T | 14.0% | 174.0 / 17.1 | 64 | A295A (GCC→GCT) | hflK | modulator for HflB protease specific for phage lambda cII repressor |
| Reads supporting (aligned to +/- strand): ref base C (24/31); new base T (4/5); total (28/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCAACGAAGTTCAGCCGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAATATAAAGCCG > minE/2864514‑2864651 | ccAACGAAGTTCAGCCGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGcc < 1:2760352/71‑1 (MQ=255) aaCGAAGTTCAGCCGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCa < 1:2064772/71‑1 (MQ=255) cAGCCGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCt > 1:3344935/1‑71 (MQ=255) cAGCCGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCt > 1:1285499/1‑71 (MQ=255) ccGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCTgg > 1:3370000/1‑70 (MQ=255) ccGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCTgg > 1:2184930/1‑70 (MQ=255) ccGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCTgg > 1:1441571/1‑70 (MQ=255) tGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCTGGAAGCTc < 1:2158384/71‑1 (MQ=255) tGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCTGGAAGCTc < 1:370184/71‑1 (MQ=255) tGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCTGGAAGCTc < 1:4013714/71‑1 (MQ=255) tGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCTGGAAGCTc < 1:1995691/71‑1 (MQ=255) cGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCTGGAAGCTCa < 1:532261/70‑1 (MQ=255) cGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCTCAGACCATCCTGGAAGCTCAGGGTg < 1:4040995/71‑1 (MQ=255) cGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCTCAGACCATCCTGGAAGCTCAGGGTg < 1:1952255/71‑1 (MQ=255) cGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCTCAGACCATCCTGGAAGCTCAGGGTg < 1:1483321/71‑1 (MQ=255) cGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCTCAGACCATCCTGGAAGCTCAGGGTg < 1:1065807/71‑1 (MQ=255) cGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCTCAGACCATCCTGGAAGCTCAGGGTg < 1:2078050/71‑1 (MQ=255) cGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCTGGAAGCTCAGGGTg < 1:879235/71‑1 (MQ=255) ggTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGa < 1:1381319/71‑1 (MQ=255) tCAGGCGCAACGTATCCTCGAAGCGGCGCGTGCGTACAAGGCCCAGACCATCCTGGAAGCTCAgg > 1:715833/1‑65 (MQ=255) tCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCTGGAAGCTCAgg > 1:1068174/1‑65 (MQ=255) tCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCTGGAAGCTCAgg > 1:2490352/1‑65 (MQ=255) tCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCTGGAAGCTCAgg > 1:75109/1‑65 (MQ=255) tCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCTGGAAGCTCAgg > 1:1554845/1‑65 (MQ=255) tCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGAAg > 1:3232403/1‑71 (MQ=255) cGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCa > 1:2542778/1‑40 (MQ=255) cGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCTgg > 1:3103890/1‑46 (MQ=255) cGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCTGGAAGCTCAg > 1:2476451/1‑54 (MQ=255) cGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCTGGAAGCTCAGGGTg < 1:3111884/58‑1 (MQ=255) cGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCtt < 1:849785/71‑1 (MQ=255) cGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCtt < 1:3840120/71‑1 (MQ=255) cGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCtt < 1:2889028/71‑1 (MQ=255) cGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCtt < 1:4022322/71‑1 (MQ=255) cGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCtt < 1:2042699/71‑1 (MQ=255) tATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGaa > 1:2538457/1‑58 (MQ=255) tATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGaa > 1:3996691/1‑58 (MQ=255) tATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGaa > 1:3535501/1‑58 (MQ=255) tATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGaa > 1:1036279/1‑58 (MQ=255) tATCCTCGAAGAGGAGCGTGCGTACAAGGCCCAGACCATCCTGGAAGCTCAggg > 1:3844344/1‑54 (MQ=255) tCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGa < 1:1453353/55‑1 (MQ=255) tCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGa < 1:3643733/55‑1 (MQ=255) cTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCTCGCTTTGCTa < 1:1367011/70‑1 (MQ=255) cTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTa < 1:1707157/70‑1 (MQ=255) gAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAAc < 1:2678219/70‑1 (MQ=255) gAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACt < 1:4039567/71‑1 (MQ=255) gAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACt < 1:913597/71‑1 (MQ=255) gAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACt < 1:1057811/71‑1 (MQ=255) tgcgtACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGaa < 1:2401120/40‑1 (MQ=255) tgcgtACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGaa < 1:1633751/40‑1 (MQ=255) tgcgtACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGaa < 1:1641408/40‑1 (MQ=255) tgcgtACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGaa < 1:1662008/40‑1 (MQ=255) tgcgtACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGaa < 1:555550/40‑1 (MQ=255) tgcgtACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGaa < 1:1675678/40‑1 (MQ=255) tgcgtACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGaa < 1:2606053/40‑1 (MQ=255) tgcgtACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGaa < 1:3875039/40‑1 (MQ=255) tgcgtACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGaa < 1:3713176/40‑1 (MQ=255) tgcgtACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCtt > 1:3682442/1‑51 (MQ=255) tgcgtACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGaa > 1:2942485/1‑70 (MQ=255) cgtACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAata > 1:2418086/1‑70 (MQ=255) cAAGGCTCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAATATAAAg > 1:4053434/1‑71 (MQ=255) cAAGGCTCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAATATAAAg > 1:2926478/1‑71 (MQ=255) cAAGGCTCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAATATAAAg > 1:1190016/1‑71 (MQ=255) aaGGCTCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAATATAAAGc > 1:1839895/1‑71 (MQ=255) ggCCCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTg > 1:3664645/1‑44 (MQ=255) ggCCCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAATATAAAGCcg > 1:3517229/1‑71 (MQ=255) | CCAACGAAGTTCAGCCGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAATATAAAGCCG > minE/2864514‑2864651 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |