| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,912,429 | C→T | 35.9% | A135T (GCG→ACG) | pyrI ← | aspartate carbamoyltransferase, regulatory subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,912,429 | 0 | C | T | 35.9% | 97.1 / 92.3 | 92 | A135T (GCG→ACG) | pyrI | aspartate carbamoyltransferase, regulatory subunit |
| Reads supporting (aligned to +/- strand): ref base C (34/25); new base T (23/10); total (57/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.73e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 101 total aligned reads displayed.
CCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAG > minE/2912360‑2912493 | ccAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAgcgc < 1:2064041/70‑1 (MQ=255) aCCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGTGata > 1:964516/1‑71 (MQ=255) aCCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGTGata > 1:3590656/1‑71 (MQ=255) ttaattGGCCAGCACCACATTATGGGAAAACTCTTTTTCACATTATTTGCATTTGAGCGCGatat < 1:1260040/65‑1 (MQ=255) ttaattGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGTGATATCAt < 1:1616209/68‑1 (MQ=255) ttaattGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGTGATATCAt < 1:2290086/68‑1 (MQ=255) ttaattGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGTGATATCAt < 1:2191697/68‑1 (MQ=255) ttaattGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATc < 1:1139186/66‑1 (MQ=255) ttaattGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTgg > 1:329704/1‑71 (MQ=255) ttaattGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTgg > 1:324886/1‑71 (MQ=255) ttaattGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTgg > 1:2904254/1‑71 (MQ=255) ttaattGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTgg > 1:1385635/1‑71 (MQ=255) taattGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGgc > 1:4117360/1‑71 (MQ=255) aattGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGTGATATCATTg > 1:522943/1‑68 (MQ=255) aattGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGTGATATCATTg > 1:2707443/1‑68 (MQ=255) aattGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGTGATATCATTg > 1:2551459/1‑68 (MQ=255) aattGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCAt > 1:2678439/1‑66 (MQ=255) aattGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCAt > 1:1157822/1‑66 (MQ=255) aattGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTAGCATTTGAGCGTGATATCATTg > 1:1061596/1‑68 (MQ=255) ggCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCTATATCATTGgcgc > 1:2017634/1‑68 (MQ=255) ggCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGtt > 1:2437108/1‑71 (MQ=255) ggCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGtt > 1:2879982/1‑71 (MQ=255) ggCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGtt > 1:2445943/1‑71 (MQ=255) gCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGata < 1:3152953/57‑1 (MQ=255) gCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGata < 1:3675213/57‑1 (MQ=255) gCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGata < 1:632041/57‑1 (MQ=255) cAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGTGATATCAt < 1:854705/59‑1 (MQ=255) gCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCg > 1:1304614/1‑70 (MQ=255) accacATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATc < 1:1988123/53‑1 (MQ=255) ccacATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAgcgc < 1:1808550/46‑1 (MQ=255) cacaTTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAgcgc < 1:3590284/45‑1 (MQ=255) cacaTTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAgcgc < 1:383145/45‑1 (MQ=255) cacaTTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAgcgc < 1:2709613/45‑1 (MQ=255) cacaTTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAgcgc < 1:400074/45‑1 (MQ=255) cacaTTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAgcgc < 1:3322964/45‑1 (MQ=255) cacaTTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAgcgc < 1:1368700/45‑1 (MQ=255) cacaTTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAgcgc < 1:970115/45‑1 (MQ=255) acaTTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGTGATATCATTGGCGCGTTTTCGCACGGc < 1:138714/71‑1 (MQ=255) acaTTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACgg > 1:784327/1‑70 (MQ=255) acaTTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACgg > 1:2588901/1‑70 (MQ=255) acaTTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACgg > 1:1129937/1‑70 (MQ=255) acaTTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACgg > 1:1138894/1‑70 (MQ=255) acaTTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACgg > 1:1931202/1‑70 (MQ=255) attattGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGatat < 1:1529221/47‑1 (MQ=255) aTTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGTGa < 1:645232/44‑1 (MQ=255) ttATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCAtt < 1:1642185/50‑1 (MQ=255) ttATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCAtt < 1:1778403/50‑1 (MQ=255) ttATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCAtt < 1:308301/50‑1 (MQ=255) tATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGTGata > 1:8942/1‑44 (MQ=255) tATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGTGata > 1:3990477/1‑44 (MQ=255) tATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGTGata > 1:1831537/1‑44 (MQ=255) ggAAAACTCTTTTTCACAGTATTTGCATTTGAGCGTGATATCa > 1:3623444/1‑43 (MQ=255) ggAAAACTCTTTTTCACAGTATTTGCATTTGAGCGTGATATCa > 1:3257843/1‑43 (MQ=255) gAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGa > 1:3705553/1‑37 (MQ=255) gAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGa > 1:287165/1‑37 (MQ=255) aaaCTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGtttt > 1:2347275/1‑52 (MQ=255) aaCTCTTTTTCACATTATTTGCATTTGAGCGTGATATc < 1:441861/38‑1 (MQ=38) aCTCTTTTTCACAGTATTTGCATTTGAGCGTGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGATGaa < 1:3686885/71‑1 (MQ=255) ctctTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGATGaaa < 1:3308653/71‑1 (MQ=255) ctTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGa > 1:2990516/1‑64 (MQ=255) ctTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGa > 1:2252724/1‑64 (MQ=255) tttttCACAGTATTTGCATTTGAGCGTGATATCATTGGCGCGTTTTCGc > 1:2409409/1‑49 (MQ=255) tttttCACAGTATTTGCATTTGAGCGTGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGATGAAACCg > 1:3317074/1‑71 (MQ=255) tttttCACAGTATTTGCATTTGAGCGTGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGATGAAACCg > 1:3508241/1‑71 (MQ=255) tttCACAGTATTTGCATTTGAGCGTGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGATGAAACCgg < 1:3618520/70‑1 (MQ=255) tttCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGATGAAACCgg < 1:1191959/70‑1 (MQ=255) caGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGATGAAACCGGTTCGGc < 1:2010960/71‑1 (MQ=255) tATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGATGAAACCGGTTCgg < 1:1538575/67‑1 (MQ=255) tATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGATGAAACCGGTTCgg < 1:1408860/67‑1 (MQ=255) tATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGATGAAACCGGTTCGGCAt < 1:4030871/70‑1 (MQ=255) tATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGATGAAACCGGTTCGGCAt < 1:1588314/70‑1 (MQ=255) tATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGATGAAACCGGTTCGGCAt < 1:3820367/70‑1 (MQ=255) aTTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCg < 1:872847/37‑1 (MQ=255) aTTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCg < 1:2702091/37‑1 (MQ=255) tttGCATTTGAGCGTGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGATGAAACCGGTTCGGCATGGc > 1:1472507/1‑71 (MQ=255) tttGCATTTGAGCGTGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGATGAAACCGGTTCGGCATGGc > 1:1140248/1‑71 (MQ=255) tttGCATTTGAGCGTGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGATGAAACCGGTTCGGCATGGc > 1:3161124/1‑71 (MQ=255) tttGCATTTGAGCGTGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGATGAAACCGGTTCGGCATGGc > 1:1872261/1‑71 (MQ=255) tttGCATTTGAGCGTGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGATGAAACCGGTTCGGCATGGc > 1:3451941/1‑71 (MQ=255) tttGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGATGAAACCGGTTCGGCATGGc > 1:1362155/1‑71 (MQ=255) tttGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGATGAAACCGGTTCGGCATGGc > 1:2331646/1‑71 (MQ=255) ttGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCa > 1:3048840/1‑37 (MQ=255) ttGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCa > 1:3354939/1‑37 (MQ=255) tGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGATGAAACCGGTTCGGCATGGCt < 1:1212955/70‑1 (MQ=255) tGCATTTGAGCGCGATATCATTGGCGCCTTTTCGCACGGC‑AAAGCTGGATGAAACCGGTTCGGCATGGCt < 1:3864400/70‑1 (MQ=255) tttGAGCGTGATATCATTGGCGCGTTTTCGCACGGC‑AAAGc < 1:2263400/41‑1 (MQ=255) tttGAGCGCGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTg < 1:3900055/43‑1 (MQ=255) tttGAGCGCGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGATGAAACCGGTTCGGCATGGCTGATAc < 1:2954653/71‑1 (MQ=255) tttGAGCGCGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGATGAAACCGGTTCGGCATGGCTGATAc < 1:1523787/71‑1 (MQ=255) ttGAGCGTGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGATGAAACCGGTTCGGCATGGCTGATa > 1:492105/1‑69 (MQ=255) ttGAGCGTGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGATGAAACCGGTTCGGCATGGCTGATa > 1:3705256/1‑69 (MQ=255) ttGAGCGTGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGATGAAACCGGTTCGGCATGGCTGATa > 1:71025/1‑69 (MQ=255) ttGAGCGTGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGATGAAACCGGTTCGGCATGGCTGATa > 1:3491964/1‑69 (MQ=255) ttGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAAGCTGGATGAAACCGGTTCGGCATg > 1:1706521/1‑63 (MQ=255) ttGAGCGCGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGATGAAACCGGTTCGGCATGGCTGATa > 1:2189622/1‑69 (MQ=255) tGAGCGCGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGATGAAACCgg > 1:801604/1‑52 (MQ=255) tGAGCGCGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGATGAAACCgg > 1:2096147/1‑52 (MQ=255) tGAGCGCGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGATGAAACCgg > 1:3580398/1‑52 (MQ=255) tGAGCGCGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGATGAAACCgg > 1:3886516/1‑52 (MQ=255) tGAGCGCGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAg > 1:1338453/1‑71 (MQ=255) | CCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAG > minE/2912360‑2912493 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |