| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,948,123 | T→C | 21.4% | T40A (ACC→GCC) | yjjB ← | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,948,123 | 0 | T | C | 21.4% | 114.5 / 28.3 | 56 | T40A (ACC→GCC) | yjjB | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (20/24); new base C (4/8); total (24/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.25e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTTTTACCGCCGAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGTACCGACCAGCATAG > minE/2948054‑2948188 | tttttACCGCCGAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTa < 1:518001/71‑1 (MQ=255)tttttACCGCCGAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTa < 1:496285/71‑1 (MQ=255)tttttACCGCCGAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTa < 1:411873/71‑1 (MQ=255) ttttACCGCCGAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTa < 1:2938057/70‑1 (MQ=255) ccgccgAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGCAAaca > 1:2661766/1‑69 (MQ=255) ccgccgAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGCAAACACt > 1:642868/1‑71 (MQ=255) aaaTCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGa < 1:3538970/71‑1 (MQ=255) ttGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGcc < 1:3212597/71‑1 (MQ=255) gCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCa < 1:1166526/70‑1 (MQ=255) gCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGCCCGCCACGGTAAACACTTTCGGATGCGCCa < 1:1372336/70‑1 (MQ=255) cGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGa < 1:3742606/71‑1 (MQ=255) gTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTa < 1:3684302/48‑1 (MQ=255) gTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTa < 1:2957219/48‑1 (MQ=255) tataAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCg < 1:4012228/57‑1 (MQ=255) tataAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCg < 1:1818886/57‑1 (MQ=255) tataAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATAc < 1:1711145/71‑1 (MQ=255) aaGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCa < 1:2952528/63‑1 (MQ=255) gCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGCAAACACTTTCGGATGCGCCAGATACCAgcg > 1:3875810/1‑71 (MQ=255) tatGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGAcc < 1:2816640/71‑1 (MQ=255) tatGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGAc > 1:3657785/1‑70 (MQ=255) tatGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGAc > 1:678507/1‑70 (MQ=255) tatGCCCGGGAACATAGGGATAACGGCCGCCACGGCAAACACTTTCGGATg > 1:3880376/1‑51 (MQ=255) tatGCCCGGGAACATAGGGATAACGGCCGCCACGGCAAACACTTTCGGATGCGCCAGATACCAGCGCGAcg < 1:1255878/71‑2 (MQ=255) tatGCCCGGGAACATAGGGATAACGGCCGCCACGGCAAACACTTTCGGATGCGCCAGATACCAGCGCGAcc < 1:3180287/71‑1 (MQ=255) cccGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTg < 1:882575/71‑1 (MQ=255) aaCATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATAc > 1:2833473/1‑70 (MQ=255) aTAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAAt < 1:2227611/71‑1 (MQ=255) aGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCaa > 1:3007944/1‑68 (MQ=255) gggATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCaa > 1:378934/1‑67 (MQ=255) gggATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATgg > 1:3961345/1‑70 (MQ=255) gggATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATgg > 1:1439498/1‑70 (MQ=255) gATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGTAc > 1:2155132/1‑71 (MQ=255) tAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGaa < 1:1078935/57‑1 (MQ=255) tAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGTAcc > 1:2578332/1‑70 (MQ=255) tAACGGCCGCCACGGCAAACACTTTCGGATGCGCCAGATAc < 1:779895/41‑1 (MQ=255) aCGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATa > 1:3480681/1‑57 (MQ=255) aCGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGTACCGAc < 1:2528021/71‑1 (MQ=255) aCGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGTACCGAc > 1:781231/1‑71 (MQ=255) aCGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGTACCGAc > 1:2266861/1‑71 (MQ=255) aCGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGTACCGAc < 1:557589/71‑1 (MQ=255) aCGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGTACCGAc < 1:74928/71‑1 (MQ=255) gccgccACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGTACCGACCAg > 1:57997/1‑71 (MQ=255) gccgccACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGTACCGACCAg < 1:3485719/71‑1 (MQ=255) gccgccACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGTACCGACCAg > 1:3307186/1‑71 (MQ=255) cgccACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGTACCGACCAGCa > 1:3155519/1‑71 (MQ=255) cgccACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGTACCGACCAGCa > 1:831791/1‑71 (MQ=255) gccACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGTACCGACCAGCa > 1:1964098/1‑70 (MQ=255) gccACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGTACCGACCAGCa > 1:3232811/1‑70 (MQ=255) gccACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGTACCGACCAGCa > 1:658575/1‑70 (MQ=255) cACGGCAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGTACCGACCAGCATAg < 1:1386669/71‑1 (MQ=255) cACGGCAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGTACCGACCAGCATAg < 1:3992100/71‑1 (MQ=255) cACGGCAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGTACCGACCAGCATAg < 1:2918722/71‑1 (MQ=255) cACGGCAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGTACCGACCAGCATAg < 1:2768833/71‑1 (MQ=255) cACGGCAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGTACCGACCAGCATAg < 1:2407658/71‑1 (MQ=255) aCGGTAAACACTTTCGGATGCGCCAGATACCATCGCGACCATTGAATACCAATGGTACCGACCAGCATAg > 1:1881178/1‑70 (MQ=255) cGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATAcc < 1:2078192/49‑1 (MQ=255) | TTTTTACCGCCGAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGTACCGACCAGCATAG > minE/2948054‑2948188 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |