| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,986,542 | A→G | 26.9% | L19L (TTG→CTG) | arcA ← | DNA‑binding response regulator in two‑component regulatory system with ArcB or CpxA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,986,542 | 0 | A | G | 26.9% | 153.8 / 68.4 | 93 | L19L (TTG→CTG) | arcA | DNA‑binding response regulator in two‑component regulatory system with ArcB or CpxA |
| Reads supporting (aligned to +/- strand): ref base A (31/37); new base G (23/2); total (54/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.66e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.20e-01 | |||||||||||
ATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCT > minE/2986477‑2986607 | aTGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAGCgtgt > 1:3349668/1‑71 (MQ=255)aTGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACgt < 1:127093/69‑1 (MQ=255)aTGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACgt < 1:2437159/69‑1 (MQ=255) gCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTg > 1:730495/1‑71 (MQ=255) gCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTg > 1:3133693/1‑71 (MQ=255) gCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTg > 1:1530949/1‑71 (MQ=255) cATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGc < 1:3864833/71‑1 (MQ=255) cATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGc < 1:3440147/71‑1 (MQ=255) tttCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCgt < 1:748866/71‑1 (MQ=255) tttCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCgt < 1:1915803/71‑1 (MQ=255) tttCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCgt < 1:2086428/71‑1 (MQ=255) tCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCgtgt > 1:2146222/1‑71 (MQ=255) tCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCgtgt > 1:3460198/1‑71 (MQ=255) tCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCgtgt > 1:4034063/1‑71 (MQ=255) tCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCgtgt > 1:2922255/1‑71 (MQ=255) tCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCgtg > 1:3328155/1‑70 (MQ=255) gcCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTAcc < 1:122861/70‑1 (MQ=255) ccATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCg < 1:480628/62‑1 (MQ=255) ccATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCg < 1:654377/62‑1 (MQ=255) aTCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTg < 1:288654/58‑1 (MQ=255) aTCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTg < 1:3238334/58‑1 (MQ=255) aTCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTg < 1:3000963/58‑1 (MQ=255) aTCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTg < 1:3650283/58‑1 (MQ=255) aTCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTg < 1:604737/58‑1 (MQ=255) tCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAAc < 1:33637/51‑1 (MQ=255) tCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAAc < 1:113461/51‑1 (MQ=255) tCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACgt < 1:591691/53‑1 (MQ=255) cTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCg > 1:1435736/1‑58 (MQ=255) cTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCg > 1:2960818/1‑58 (MQ=255) cTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTT‑AACGTGTTGCg > 1:589909/1‑57 (MQ=255) tGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAGCGTGTTGCGTGTTACCAACTCGt > 1:1619536/1‑71 (MQ=255) tGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAGCGTGTTGCGTGTTACCAACTCGt > 1:2072116/1‑71 (MQ=255) tGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAGCGTGTTGCGTGTTACCAACTCGt > 1:2906887/1‑71 (MQ=255) tGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAGCGTGTTGCGTGTTACCAACTCGt > 1:2109062/1‑71 (MQ=255) tGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAGCGTGTTGCGTGTTACCAACTCGt > 1:529350/1‑71 (MQ=255) tGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAGCGTGTTGCGTGTTACCAACTCGt > 1:3270173/1‑71 (MQ=255) tGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCgtgt > 1:1425516/1‑60 (MQ=255) tGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGt > 1:3901645/1‑71 (MQ=255) tGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGt > 1:1499442/1‑71 (MQ=255) ttCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACgt < 1:3962713/45‑1 (MQ=255) cGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACg > 1:320246/1‑42 (MQ=255) cGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAAc > 1:2695856/1‑70 (MQ=255) cGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAAc > 1:2741717/1‑70 (MQ=255) gAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAAc < 1:33475/69‑1 (MQ=255) aaCATCATAGCCTTCCGCTTCGAAAATACTTTTCAGCGTGTTGCGTGTTACCAACTCGTCTTCAACGAt > 1:1422590/1‑69 (MQ=255) aaCATCATAGCCTTCCGCTTCGAAAATACTTTTCAGCGTGTTGCGTGTTACCAACTCGTCTTCAACGATaa < 1:373144/71‑1 (MQ=255) aaCATCATAGCCTTCCGCTTCGAAAATACTTTTCAGCGTGTTGCGTGTTACCAACTCGTCTTCAACGATaa < 1:2104695/71‑1 (MQ=255) aCATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAg < 1:3392294/71‑1 (MQ=255) aCATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAg < 1:597251/71‑1 (MQ=255) catcatAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCgtgt < 1:2688503/46‑1 (MQ=255) catcatAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCgtgt < 1:108758/46‑1 (MQ=255) catcatAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCgtgt < 1:2683655/46‑1 (MQ=255) catcatAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCgtgt < 1:1400561/46‑1 (MQ=255) atcatAGCCTTCCGCTTCGAAAATACTTTTCAGCGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGaa > 1:801585/1‑71 (MQ=255) atcatAGCCTTCCGCTTCGAAAATACTTTTCAGCGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGaa > 1:929452/1‑71 (MQ=255) tcatAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAAc < 1:2116392/51‑1 (MQ=255) tcatAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAAc < 1:1467486/51‑1 (MQ=255) tcatAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAAc < 1:3438864/51‑1 (MQ=255) atAGCCTTCCGCTTCGAAAATACTTTTCAGCGTGTTGCGTGTTACCAACTCGTCTTCAACGAt > 1:1396040/1‑63 (MQ=255) atAGCCTTCCGCTTCGAAAATACTTTTCAGCGTGTTGCGTGTTACCAACTCGTCTTCAACGAt > 1:3727011/1‑63 (MQ=255) atAGCCTTCCGCTTCGAAAATACTTTTCAGCGTGTTGCGTGTTACCAACTCGTCTTCAACGAt > 1:4101962/1‑63 (MQ=255) atAGCCTTCCGCTTCGAAAATACTTTTCAGCGTGTTGCGTGTTACCAACTCGTCTTCAACGAt > 1:2562240/1‑63 (MQ=255) atAGCCTTCCGCTTCGAAAATACTTTTCAGCGTGTTGCGTGTTACCAACTCGTCTTCAACGAt > 1:1922181/1‑63 (MQ=255) atAGCCTTCCGCTTCGAAAATACTTTTCAGCGTGTTGCGTGTTACCAACTCGTCTTCAACGAt > 1:238395/1‑63 (MQ=255) atAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCaa > 1:3426422/1‑48 (MQ=255) atAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTc > 1:933471/1‑51 (MQ=255) atAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTc > 1:1689070/1‑51 (MQ=255) tCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGAt < 1:561701/56‑1 (MQ=255) cTTCGAAAATACTTTTCAGCGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCt > 1:2190445/1‑69 (MQ=255) cTTCGAAAATACTTTTCAGCGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTg > 1:146945/1‑70 (MQ=255) ttCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGATtgt < 1:3895908/59‑1 (MQ=255) ttCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAAtgt < 1:1660249/59‑1 (MQ=255) ttCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAAtgt < 1:4016047/59‑1 (MQ=255) ttCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAAtgt < 1:3458697/59‑1 (MQ=255) tCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCAt > 1:1566480/1‑71 (MQ=255) cGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATg > 1:1882984/1‑71 (MQ=255) gAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGt < 1:1574541/71‑1 (MQ=255) gAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGt < 1:1750901/71‑1 (MQ=255) aaaaTACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGt < 1:920523/70‑1 (MQ=255) aaTACTTTTCAGCGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGttt > 1:3036402/1‑70 (MQ=255) aaTACTTTTCAGCGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGttt > 1:3811780/1‑70 (MQ=255) aaTACTTTTCAGCGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTg > 1:1776614/1‑71 (MQ=255) aaTACTTTTCAGCGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTg > 1:1234199/1‑71 (MQ=255) aaTACTTTTCAGCGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTg > 1:504077/1‑71 (MQ=255) aaTACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTg > 1:784277/1‑71 (MQ=255) aaTACTGTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTg > 1:7519/1‑71 (MQ=255) aCTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTa > 1:3934187/1‑71 (MQ=255) ttCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCa > 1:3444732/1‑58 (MQ=255) ttCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCa > 1:764521/1‑58 (MQ=255) ttCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCt > 1:1390384/1‑70 (MQ=255) ttCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCt > 1:1385249/1‑70 (MQ=255) ttCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCt > 1:3790528/1‑70 (MQ=255) ttCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGCGTCTGCa > 1:3435459/1‑58 (MQ=255) aaCGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAg < 1:3577145/38‑1 (MQ=255) | ATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCT > minE/2986477‑2986607 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |