| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 298,537 | C→T | 38.2% | L400L (CTG→TTG) | mdlA → | fused predicted multidrug transporter subunits and ATP‑binding components of ABC superfamily |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 298,537 | 0 | C | T | 38.2% | 91.0 / 114.0 | 102 | L400L (CTG→TTG) | mdlA | fused predicted multidrug transporter subunits and ATP‑binding components of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base C (33/30); new base T (23/16); total (56/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.45e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.68e-01 | |||||||||||
Only 100 of 110 total aligned reads displayed.
TTGTCGCTCATTCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTAGCCAGACGCCATTCCTTTTT > minE/298474‑298605 | ttgtCGCTCATTCAGCGTCATTTCGACGTCAGCGAGTGGGATATTCGCTTTCATGATATTCCTCTGACGa < 1:1101833/70‑1 (MQ=255)ttgtCGCTCATTCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGa < 1:4072596/70‑1 (MQ=255)ttgtCGCTCATTCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGa < 1:1600823/70‑1 (MQ=255)ttgtCGCTCATTCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGa < 1:1339979/70‑1 (MQ=255) tgtCGCTCATTCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGAAg > 1:777050/1‑71 (MQ=255) tgtCGCTCATTCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGAAg > 1:772310/1‑71 (MQ=255) gtCGCTCATTCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGAAg > 1:1071133/1‑70 (MQ=255) gtCGCTCATTCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGAAg > 1:1105478/1‑70 (MQ=255) gtCGCTCATTCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGAAg > 1:3152256/1‑70 (MQ=255) tCGCTCATTCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGa < 1:2887870/67‑1 (MQ=255) tCATTCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGAAGtt < 1:2073576/67‑1 (MQ=255) aTTCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGAAGTTa < 1:1618351/66‑1 (MQ=255) aTTCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGAAGTTa < 1:490096/66‑1 (MQ=255) aTTCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGAAGTTa < 1:2940681/66‑1 (MQ=255) ttCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGAAGTTACAACTc > 1:3315065/1‑71 (MQ=255) aGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTTTGACGAAGTTACAACTCGAt < 1:726111/71‑1 (MQ=255) aGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTTTGACGAAGTTACAACTCGAt < 1:3566382/71‑1 (MQ=255) gTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGAAGTTACAACTCGATAGc > 1:3051200/1‑71 (MQ=255) gTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGAAGTTACAACTCGATAGc > 1:1889932/1‑71 (MQ=255) tCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTTTGACGAAGTTAc < 1:2464587/59‑1 (MQ=255) cATTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTTTGACGAAGTTACAACTCGATAGCTg > 1:3585193/1‑71 (MQ=255) aTTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTTTGACGAAGTTACaa > 1:1313157/1‑59 (MQ=255) aTTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTTTGACGAAGTTACaa > 1:3110284/1‑59 (MQ=255) aTTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTTTGACGAAGTTACaa > 1:752362/1‑59 (MQ=255) aTTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTTTGACGAAGTTACaa > 1:3681458/1‑59 (MQ=255) aTTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTTTGACGAAGTTACaa > 1:3455183/1‑59 (MQ=255) aTTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGAAGTTACAACTCGATAGCTgg > 1:377416/1‑71 (MQ=255) aTTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGAAGTTACAACTCGATAGCTgg > 1:3009271/1‑71 (MQ=255) ttCGACTTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGa > 1:535816/1‑46 (MQ=255) ttCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGa > 1:1908321/1‑46 (MQ=255) ttCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGa > 1:212892/1‑46 (MQ=255) ttCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGa > 1:3923912/1‑46 (MQ=255) ttCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGa > 1:885513/1‑46 (MQ=255) ttCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGa > 1:972151/1‑46 (MQ=255) cGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTTTGACGAAGTTACAACTCGATAGCTGGc > 1:630770/1‑68 (MQ=255) cGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTTTGACGAAGTTACAACTCGATAGCTGGCg > 1:3200786/1‑69 (MQ=255) cGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTTTGACGAAGTTACAACTCGATAGCTGGCg > 1:3172585/1‑69 (MQ=255) cGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTTTGACGAAGTTACAACTCGATAGCTGGCg > 1:1259782/1‑69 (MQ=255) cGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTTTGACGAAGTTACAACTCGATAGCTGGCg > 1:2937739/1‑69 (MQ=255) gACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGAAGTTACAACTCGATAGCTGGCGTa < 1:3012542/70‑1 (MQ=255) cGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGAAGTTACAACTCGATAGCTGGCGTa < 1:2966718/68‑1 (MQ=255) tCAGCGAGGGGGATATTCGCTTTCATGATATTCCTTTGACGAAGTTACAACTCGATAGCTGGCGt > 1:3949/1‑65 (MQ=255) tCAGCGAGGGGGATATTCGCTTTCATGATATTCCTTTGACGAAGTTACAACTCGATAGCTGGCGTAgccgc > 1:1740881/1‑71 (MQ=255) tCAGCGAGGGGGATATTCGCTTTCATGATATTCCTTTGACGAAGTTACAACTCGATAGCTGGCGTAgccgc > 1:42607/1‑71 (MQ=255) tCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGAAGTTACAACTc < 1:4046826/53‑1 (MQ=255) tCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGAAGTTACAACTc < 1:2943754/53‑1 (MQ=255) tCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGAAGTTACAACTc < 1:3985201/53‑1 (MQ=255) tCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGAAGTTACAACTc < 1:120009/53‑1 (MQ=255) tCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGAAGTTACAACTc < 1:3367902/53‑1 (MQ=255) cAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGAAGTTACAACTCGATAGCTGGCGTAgccgc < 1:2019847/70‑1 (MQ=255) aGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGAAGTTACAACTCGAt < 1:1122169/54‑1 (MQ=255) aGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCt > 1:51976/1‑71 (MQ=255) gggATATTCGCTTTCATGATATTCCTCTGACGAAGTTACAACTCGATAg > 1:266634/1‑49 (MQ=255) gggATATTCGCTTTCATGATATTCCTCTGACGAAGTTACAACTCGATAGCTGGCGTAgc > 1:2863673/1‑59 (MQ=255) gggATATTCGCTTTCATGATATTCCTCTGACGAAGTTACAACTCGATAGCTGGCGTAgc > 1:25658/1‑59 (MQ=255) atatTCGCTTTCATGATATTCCTCTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGtt < 1:1671048/71‑1 (MQ=255) atTCGCTTTCATGATATTCCTCTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTAg > 1:4087526/1‑71 (MQ=255) atTCGCTTTCATGATATTCCTCTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTAg > 1:3467381/1‑71 (MQ=255) ttCGCTTTCATGATATTCCTTTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTgg < 1:946438/60‑1 (MQ=255) ttCGCTTTCATGATATTCCTTTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTgg < 1:2128297/60‑1 (MQ=255) ttCGCTTTCATGATATTCCTTTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTa > 1:1307218/1‑69 (MQ=255) ttCGCTTTCATGATATTCCTTTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTa > 1:103699/1‑69 (MQ=255) ttCGCTTTCATGATATTCCTTTGACGAAGTTACAACTCGATACCTGGCGTAGCCGCCTGGCGGTAGTTa > 1:3118623/1‑69 (MQ=255) ttCGCTTTCATGATATTCCTCTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTAGc < 1:3150811/71‑1 (MQ=255) cGCTTTCATGATATTCCTTTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCg > 1:3312828/1‑60 (MQ=255) cGCTTTCATGATATTCCTTTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTAGc > 1:2742199/1‑69 (MQ=255) cGCTTTCATGATATTCCTTTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTAGc > 1:2661535/1‑69 (MQ=255) cGCTTTCATGATATTCCTCTGACGAAGTTACAACTCGa > 1:1161401/1‑38 (MQ=255) cTTTCATGATATTCCTCTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTAGCCa < 1:3323711/69‑1 (MQ=255) tttCATGATATTCCTTTGACGAAGTTACAACTCGATAGCTgg < 1:2184029/42‑1 (MQ=255) tttCATGATATTCCTTTGACGAAGTTACAACTCGATAGCTgg < 1:2070414/42‑1 (MQ=255) cATGATATTCCTCTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTAGCCAGACGc > 1:2531056/1‑70 (MQ=255) cATGATATTCCTCTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTAGCCAGACGc > 1:1164300/1‑70 (MQ=255) cATGATATTCCTCTGACGAAGTCACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTAGCCAGACGc > 1:3448445/1‑70 (MQ=255) aTGATATTCCTCTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTAGCCAGACGCCa < 1:1659779/71‑1 (MQ=255) aTGATATTCCTCTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTAGCCAGACGCCa < 1:2026744/71‑1 (MQ=255) gATATTCCTTTGACGAAGTTACAACTCGATAGCTgg < 1:2073771/36‑1 (MQ=255) gATATTCCTTTGACGAAGTTACAACTCGATAGCTGGCGTa < 1:2736292/40‑1 (MQ=255) gATATTCCTTTGACGAAGTTACAACTCGATAGCTGGCGTa < 1:2713006/40‑1 (MQ=255) atatTCCTTTGACGAAGTTACAACTCGATAGCTGGCGTAgc > 1:440866/1‑41 (MQ=255) tatTCCTCTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGt < 1:103937/51‑1 (MQ=255) tatTCCTCTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGt < 1:3690677/51‑1 (MQ=255) tatTCCTCTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGt < 1:2596707/51‑1 (MQ=255) tatTCCTCTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGt < 1:365876/51‑1 (MQ=255) ttCCTTTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTAGCCAGACGCCATTCCtt < 1:166589/71‑1 (MQ=255) ttCCTTTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTAGCCAGACGCCATTCCtt < 1:1763249/71‑1 (MQ=255) ttCCTTTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTAGCCAGACGCCATTCCt < 1:3632530/70‑1 (MQ=255) ttCCTTTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTAGCCAGACGCCATTCCt < 1:2597927/70‑1 (MQ=255) ttCCTTTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTAGCCAGACGCCATTCCt < 1:3899996/70‑1 (MQ=255) ttCCTCTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTAGCCAGACGCCATTCCtt > 1:2938296/1‑71 (MQ=255) tCCTTTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTAGCCAGACGCCATTCCttt > 1:3290819/1‑71 (MQ=255) ccTCTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTAGCCAGACGCCATTCCtttt > 1:3520519/1‑71 (MQ=255) ctctGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTAGCCAGACGCCATTCCttttt < 1:784830/71‑1 (MQ=255) ctctGACGAAGTTACAACTCGATAGCTGGCGTACCCGCCTGGCGGTAGTTAGCCAGACGCCATTCCttttt < 1:406595/71‑1 (MQ=255) tctGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTAGCCAGACGc < 1:3302865/59‑1 (MQ=255) tctGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTAGCCAGACGc < 1:562397/59‑1 (MQ=255) tctGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTAGCCAGACGc < 1:2232581/59‑1 (MQ=255) tctGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTAGCCAGACGc < 1:2237308/59‑1 (MQ=255) tctGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTAGCCAGACGc < 1:2381613/59‑1 (MQ=255) tctGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTAGCCAGACGc < 1:1789377/59‑1 (MQ=255) | TTGTCGCTCATTCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTAGCCAGACGCCATTCCTTTTT > minE/298474‑298605 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |