| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 405,935 | T→G | 36.4% | Q118P (CAG→CCG) | ybeA ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 405,935 | 0 | T | G | 36.4% | 1.5 / 25.9 | 22 | Q118P (CAG→CCG) | ybeA | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (9/5); new base G (6/2); total (15/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
GACTCTCTGCGACCAGCACGCGAACCAGCGGATGGGGGAGGGTAAGCGCCGACAGCGACCAGCTCTGCTCAGCCGCCGCTTTACAGGCAGGCGACAACCCTTCAGGCCCGCCAATCAGTAGACTGACGTCGC > minE/405870‑406001 | gACTCTCTGCGACCAGCACGCGAACCAGCGGATGGGGGAGGGTAAGCGCCGACAGCGACCAGCTCTGCt > 1:2242945/1‑69 (MQ=255) ccAGCACGCGAACCAGCGGATGGGGGAGGGTAAGCCCCGACAGCGACCAGCTCGGCTCAGCCGCCGCTTTa < 1:3116815/71‑1 (MQ=255) aGCACGCGAACCAGCGGATGGTGGAGGGTAAGCGCCGACAGCGACCAGCTCTGCTCAGCCGCCGCTTTa > 1:3876984/1‑69 (MQ=255) gCACGCGAACCAGCGGATGGGGGAGGGTAAGCGCCGACAGAGACCAGCTCTGCTCAGCCGCCGCTTTAcag > 1:1513961/1‑71 (MQ=255) gATGGGTGAGGGTAAGCGCCGACAGCGACCAGCTCTGCTCAGCCGCCGCTTTACAGGCAGGCGACAACCCt < 1:372281/71‑1 (MQ=255) tGGGGGAGGGTAAGCGCCGACAGCGACCAGCTCTGCTCAGCCGCCGCTTTACAGGCAGGCGACAACCCtt > 1:4064500/1‑70 (MQ=255) tGGGGGAGGGTAAGCGCCGACAGCGACCAGCTCTGCTCAGCCGCCGCTTTACAGGCAGGCGACAACCCtt > 1:2378948/1‑70 (MQ=255) tGGGGGAGGGTAAGCGCCGACAGCGACCAGCTCTGCTCAGCCGCCGCTTTACAGGCAGGCGACAACCCtt > 1:2595762/1‑70 (MQ=255) ggggAGGGTAAGCGCCGACAGCGACCAGCTCTGCTCAGCCGCCGCTTTACAGGCAGGCGACAACCCTTCAg > 1:3824203/1‑71 (MQ=255) ggggAGGGTAAGCGCCGACAGCGACCAGCTCTGCTCAGCCGCCGCTTTACAGGCAGGCGACAACCCTTCAg > 1:2642460/1‑71 (MQ=255) ggTAAGCGCCGACAGCGACCAGCTCGGCTCAGCCGCCGCTTTACAGGCAGGCGACAACCCTTCAGGCCCGc > 1:3734960/1‑71 (MQ=255) aaGCGCCGACAGCGACCAGCTCGGCTCAGCCGCCGCTTTACAGGCAGGCGACAACCCTTCAGGCCCGCCaa > 1:1327752/1‑71 (MQ=255) aaGCGCCGACAGCGACCAGCTCGGCTCAGCCGCCGCTTTACAGGCAGGCGACAACCCTTCAGGCCCGCCaa > 1:3225300/1‑71 (MQ=255) ccGACAGCGACCAGCTCTGCTCAGCCGCCGCTTTACAGGCAGGCGACAACCCTTCAGGCCCGCCAATCAg < 1:999914/70‑1 (MQ=255) cGACAGCGACCAGCTCTGCTCAGCCGCCGCTTTACAGGCAGGCGACAACCCTTCAGGCCCGCCAATCAGTa < 1:3707298/71‑1 (MQ=255) aGCGACCAGCTCGGCTCAGCCGCCGCTTTACAGGCAGGCGACAACCCTTCAGGCCCGCCAATCAGTAGa > 1:2496241/1‑69 (MQ=255) aGCGACCAGCTCGGCTCAGCCGCCGCTTTACAGGCAGGCGACAACCCTTCAGGCCCGCCAATCAGTAGa > 1:223371/1‑69 (MQ=255) aGCGACCAGCTCGGCTCAGCCGCCGCTTTACAGGCAGGCGACAACCCTTCAGGCCCGCCAATCAGTAGa > 1:760208/1‑69 (MQ=255) aGCGACCAGCTCGGCTCAGCCGCCGCTTTACAGGCAGGCGACAACCCTTCAGGCCCGCCAATCAGTAGACt < 1:1233238/71‑1 (MQ=255) gCGACCAGCTCTGCTCAGCCGCCGCTTTACAGGCAGGCGACAACCCTTCAGGCCCGCCAATCAGTAGACt > 1:3501742/1‑70 (MQ=255) cAGCTCTGCTCAGCCGCCGCTTTACAGGCAGGCGACAACCCTTCAGGCCCGcc < 1:594413/53‑1 (MQ=255) gCTCTGCTCAGCCGCCGCTTTACAGGCAGGCGACAACCCTTCAGGCCCGCCAATCAGTAGACTGACGTcgc < 1:1288524/71‑1 (MQ=255) | GACTCTCTGCGACCAGCACGCGAACCAGCGGATGGGGGAGGGTAAGCGCCGACAGCGACCAGCTCTGCTCAGCCGCCGCTTTACAGGCAGGCGACAACCCTTCAGGCCCGCCAATCAGTAGACTGACGTCGC > minE/405870‑406001 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |