Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 447,412 G→A 58.3% R102C (CGC→TGC)  kdpD ← fused sensory histidine kinase in two‑component regulatory system with KdpE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE447,4120GA58.3% ‑0.1 / 10.8 12R102C (CGC→TGC) kdpDfused sensory histidine kinase in two‑component regulatory system with KdpE
Reads supporting (aligned to +/- strand):  ref base G (3/2);  new base A (5/2);  total (8/4)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.21e-01

TTGGGATGACGGGAACCTGGCGCATTACTGTGCGCCAGTTCGTCCATTAAGATCAGCGCCGGGCGGCGGGCGAGGGCGGCATCGAGATCAAACTCGCTGATATGCCGCCCACGGTACGCCT  >  minE/447348‑447468
                                                                |                                                        
ttGGGATGACGGGAACCTGGCGCATTACTGTGCGCCAGTTCGTCCATTAAGATCAGCGCCGGGCagcgggc                                                    >  1:2253563/1‑71 (MQ=255)
ttGGGATGACGGGAACCTGGCGCATTACTGTGCGCCAGTTCGTCCATTAAGATCAGCGCCGGGCagcgggc                                                    >  1:2324220/1‑71 (MQ=255)
ttGGGATGACGGGAACCTGGCGCATTACTGTGCGCCAGTTCGTCCATTAAGATCAGCGCCGGGCagcgggc                                                    >  1:3975892/1‑71 (MQ=255)
   ggATGACGGGAACCTGGCGCATTACTGTGCGCCAGTTCGTCCATTAAGATCAGCGCCGGGCGGCGGGCGAg                                                 <  1:3668930/71‑1 (MQ=255)
                          aCTGTGCGCCAGTTCGTCCATTAAGATCAGCGCCGGGCAGCGGGCGAGGGCGGCATCGAGATCAAACTCGc                          >  1:1038098/1‑71 (MQ=255)
                          aCTGTGCGCCAGTTCGTCCATTAAGATCAGCGCCGGGCAGCGGGCGAGGGCGGCATCGAGATCAAACTCGc                          >  1:1645731/1‑71 (MQ=255)
                          aCTGTGCGCCAGTTCGTCCATTAAGATCAGCGCCGGGCAGCGGGCGAGGGCGGCATCGAGATCAAACTCGc                          >  1:209273/1‑71 (MQ=255)
                              tgCGCCAGTTCGTCCATTAAGATCAGCGCCGGGCGGCGGGCGAGGGCGGCATCGAGATCAAACTCGCTGa                       <  1:20463/70‑1 (MQ=255)
                              tgCGCCAGTTCGTCCATTAAGATCAGCGCCGGGCAGCGGGCGAGGGCGGCATCGAGATCAAACTCGCTg                        <  1:3135729/69‑1 (MQ=255)
                                 gcCAGTTCGTCCATTAAGATCAGCGCCGGGCAGCGGGCGAGGGCGGCa                                          <  1:1993264/48‑1 (MQ=255)
                                               tAAGATCAGCGCCGGGCGGCGGGCGAGGGCGGCATCGAGATCaaa                               >  1:80889/1‑45 (MQ=255)
                                                aaGATCAGCGCCGGGCGGCGGGCGAGGGCGGCATCGAGATCAAACTCGCTGATATGCCGCCCACGGTACg     >  1:1977259/1‑70 (MQ=255)
                                                aaGATCAGCGCCGGGCGGCGGGCGAGGGCGGCATCGAGATCAAACTCGCTGATATGCCGCCCACGGTACGc    >  1:1820025/1‑71 (MQ=255)
                                                aaGATCAGCGCCGGGCAGCGGGCGAGGGCGGCATCGAGATCAAACTCGCTGATATg                   >  1:3441616/1‑56 (MQ=255)
                                                  gATCAGCGCCGGGCAGCGGGCGAGGGCGGCATCGAGATCAAACTCGCTGATATGCCGCCCACGGTACGCCt  >  1:1126652/1‑71 (MQ=255)
                                                                |                                                        
TTGGGATGACGGGAACCTGGCGCATTACTGTGCGCCAGTTCGTCCATTAAGATCAGCGCCGGGCGGCGGGCGAGGGCGGCATCGAGATCAAACTCGCTGATATGCCGCCCACGGTACGCCT  >  minE/447348‑447468

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑