| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 498,441 | C→T | 44.2% | G24S (GGT→AGT) | galE ← | UDP‑galactose‑4‑epimerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 498,441 | 0 | C | T | 44.2% | 74.7 / 221.8 | 165 | G24S (GGT→AGT) | galE | UDP‑galactose‑4‑epimerase |
| Reads supporting (aligned to +/- strand): ref base C (55/37); new base T (39/34); total (94/71) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.33e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.77e-01 | |||||||||||
Only 100 of 165 total aligned reads displayed.
AACGCTCGATAACAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAACCAGAACTCTCA > minE/498372‑498509 | aaCGCTCGATAACAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCg > 1:2160358/1‑71 (MQ=255)aaCGCTCGATAACAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCg > 1:2577727/1‑71 (MQ=255) cGCTCGATAACAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGtt > 1:126373/1‑71 (MQ=255) cGCTCGATAACAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGtt > 1:2088244/1‑71 (MQ=255) cGCTCGATAACAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGtt > 1:3536634/1‑71 (MQ=255) cGCTCGATAACAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGtt > 1:1429114/1‑71 (MQ=255) cGCTCGATAACAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGtt > 1:175166/1‑71 (MQ=255) gATAACAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGtttt > 1:1645550/1‑68 (MQ=255) gATAACAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTg < 1:3995892/69‑1 (MQ=255) tAACAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGt < 1:2112318/71‑1 (MQ=255) tAACAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGt < 1:1291462/71‑1 (MQ=255) ggCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTg < 1:2292429/71‑1 (MQ=255) ggCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTg < 1:2633337/71‑1 (MQ=255) cAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCAGTAATTGca < 1:633857/71‑1 (MQ=255) tACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGc < 1:1596124/58‑1 (MQ=255) tACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGc < 1:2251060/58‑1 (MQ=255) tACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGc < 1:890884/58‑1 (MQ=255) cGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCAGTAATTGcacaca < 1:2405054/70‑1 (MQ=255) cGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCAGTAATTGCACACAg > 1:3721998/1‑71 (MQ=255) cGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCAGTAATTGCACACAg > 1:417390/1‑71 (MQ=255) cGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCAGTAATTGCACACAg > 1:3434153/1‑71 (MQ=255) cGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTCTTTTGCAGTAATTGcacaca > 1:4089371/1‑70 (MQ=255) cGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAg > 1:355464/1‑58 (MQ=255) cGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAg > 1:3345689/1‑71 (MQ=255) cGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAg > 1:3822364/1‑71 (MQ=255) cGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAg > 1:2781695/1‑71 (MQ=255) cGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAg > 1:2273852/1‑71 (MQ=255) cGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAg > 1:3464470/1‑71 (MQ=255) cGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAg > 1:3165846/1‑71 (MQ=255) cGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAg > 1:4122374/1‑71 (MQ=255) cGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAg > 1:924377/1‑71 (MQ=255) cTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAgg > 1:61689/1‑70 (MQ=255) cTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAgg > 1:3868221/1‑70 (MQ=255) tGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAg > 1:1932635/1‑68 (MQ=255) tGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAg > 1:1774318/1‑68 (MQ=255) tGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAg > 1:3575131/1‑68 (MQ=255) cgcTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCATTAATTGCACACAGGTAt < 1:3903595/70‑1 (MQ=255) cgcTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCAGTAATTGCACACAGGTag < 1:3694701/70‑2 (MQ=255) cgcTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCAGTAATTGCACACAGGTAt < 1:2066966/70‑1 (MQ=255) cgcTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCAGTAATTCCACACAGGTAt < 1:2730034/70‑1 (MQ=255) ttACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACt > 1:3649735/1‑71 (MQ=255) tACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGt < 1:349986/50‑1 (MQ=255) tACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGt < 1:2902507/50‑1 (MQ=255) tACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTaa > 1:1402023/1‑52 (MQ=255) tACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACtt > 1:1357231/1‑71 (MQ=255) ttACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAAt > 1:1383546/1‑48 (MQ=255) ttACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCa < 1:135127/69‑1 (MQ=255) ttATCAAGAATGATGACATCATGACTGTTTTGCAGTAATTGCACACAgg < 1:3613899/49‑1 (MQ=255) ttATCAAGAATGATGACATCATGACTGTTTTGCAGTAATTGCACACAgg < 1:1420250/49‑1 (MQ=255) ttATCAAGAATGATGACATCATGACTGTTTTGCAGTAATTGCACACAgg < 1:3390177/49‑1 (MQ=255) ttATCAAGAATGATGACATCATGACTGTTTTGCAGTAATTGCACACAgg < 1:1087656/49‑1 (MQ=255) ttATCAAGAATGATGACATCATGACTGTTTTGCAGTAATTGCACACAGGt < 1:1561475/50‑1 (MQ=255) ttATCAAGAATGATGACATCATGACTGTTTTGCAGTAATTGCACACAGGt < 1:2579780/50‑1 (MQ=255) ttATCAAGAATGATGACATCATGACTGTTTTGCAGTAATTGCACACAGGt < 1:1978954/50‑1 (MQ=255) ttATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCt < 1:270508/71‑1 (MQ=255) tATCAAGAATGATGACATCATGACTGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTa > 1:216991/1‑71 (MQ=255) tATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTa > 1:3011637/1‑71 (MQ=255) tCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAgg < 1:3912066/46‑1 (MQ=255) tCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAgg < 1:53468/46‑1 (MQ=255) tCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAgg < 1:104065/46‑1 (MQ=255) tCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAgg < 1:3310179/46‑1 (MQ=255) cAAGAATGATGACATCATGACTGTTTTGCAGTAATTGCACACAGGTATGa > 1:1033957/1‑50 (MQ=255) cAAGAATGATGACATCATGACTGTTTTGCAGTAATTGCACACAGGTATGa > 1:2562978/1‑50 (MQ=255) cAAGAATGATGACATCATGACTGTTTTGCAGTAATTGCACACAGGTATGa > 1:4060922/1‑50 (MQ=255) cAAGAATGATGACATCATGACTGTTTTGCAGTAATTGCACACAGGTATGa > 1:480962/1‑50 (MQ=255) cAAGAATGATGACATCATGACTGTTTTGCAGTAATTGCACACAGGTATGa > 1:896736/1‑50 (MQ=255) cAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTacca > 1:2719310/1‑71 (MQ=255) aaGAATGATGACATCATGACTGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTaccac > 1:2382722/1‑71 (MQ=255) aaGAATGATGACATCATGACTGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTacaac > 1:698838/1‑71 (MQ=255) gAATGATGACATCATGACTGTTTTGCAGTAATTGCACACAGGTAt < 1:478365/45‑1 (MQ=255) gAATGATGACATCATGACTGTTTTGCAGTAATTGCACACAGGTAt < 1:4122702/45‑1 (MQ=255) gAATGATGACATCATGACTGTTTTGCAGTAATTGCACACAGGTAt < 1:2819689/45‑1 (MQ=255) gAATGATGACATCATGACTGTTTTGCAGTAATTGCACACAGGTAt < 1:2688626/45‑1 (MQ=255) gAATGATGACATCATGACTGTTTTGCAGTAATTGCACACAGGTAt < 1:248899/45‑1 (MQ=255) gAATGATGACATCATGACTGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCg > 1:2270549/1‑71 (MQ=255) atgatgACATCATGACTGTTTTGCAGTAATTGCACACAGGTATGa > 1:108765/1‑45 (MQ=255) atgatgACATCATGACTGTTTTGCAGTAATTGCACACAGGTATGa > 1:40599/1‑45 (MQ=255) atgatgACATCATGACTGTTTTGCAGTAATTGCACACAGGTATGa > 1:3974016/1‑45 (MQ=255) atgatgACATCATGACTGTTTTGCAGTAATTGCACACAGGTATGa > 1:3809451/1‑45 (MQ=255) atgatgACATCATGACTGTTTTGCAGTAATTGCACACAGGTATGa > 1:2684505/1‑45 (MQ=255) atgatgACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTac > 1:1903798/1‑64 (MQ=255) atgatgACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTac > 1:1991399/1‑64 (MQ=255) tgatgaCATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGt > 1:3502698/1‑70 (MQ=255) gatgaCATCATGACTGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTac > 1:2999695/1‑62 (MQ=255) gatgaCATCATGACTGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTac > 1:3256588/1‑62 (MQ=255) gatgaCATCATGACTGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTac > 1:607262/1‑62 (MQ=255) gatgaCATCATGACTGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTac > 1:2209930/1‑62 (MQ=255) gatgaCATCATGACTGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTac > 1:2227845/1‑62 (MQ=255) gatgaCATCATGACTGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTac > 1:2796695/1‑62 (MQ=255) gatgaCATCATGACTGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTac > 1:3745841/1‑62 (MQ=255) gatgaCATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTaa > 1:2885873/1‑71 (MQ=255) gatgaCATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTaa > 1:2349935/1‑71 (MQ=255) gatgaCATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTaa > 1:114707/1‑71 (MQ=255) atgaCATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAAc < 1:2645054/71‑1 (MQ=255) aCATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAAc > 1:668353/1‑68 (MQ=255) aCATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAACCAg < 1:3053754/71‑1 (MQ=255) catcatGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAACCAg < 1:3388272/70‑1 (MQ=255) tcatGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTa < 1:803793/47‑1 (MQ=255) tcatGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTa < 1:3244146/47‑1 (MQ=255) tcatGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTac < 1:2908182/55‑1 (MQ=255) | AACGCTCGATAACAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAACCAGAACTCTCA > minE/498372‑498509 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |