| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 507,425 | C→A | 15.1% | A71D (GCT→GAT) | ybhH → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 507,425 | 0 | C | A | 15.1% | 388.8 / 31.7 | 125 | A71D (GCT→GAT) | ybhH | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (52/54); new base A (11/8); total (63/62) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.19e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.97e-01 | |||||||||||
Only 100 of 127 total aligned reads displayed.
ACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATACCACGCCTAACTGCGGC > minE/507356‑507495 | aCGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCt < 1:2023637/71‑1 (MQ=255) cGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCt < 1:2533271/70‑1 (MQ=255) ggTATTGGCGGCGGTAATCCACTGCCCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGa > 1:442034/1‑71 (MQ=255) ttGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTg < 1:670289/66‑1 (MQ=255) ttGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTg < 1:429403/66‑1 (MQ=255) ttGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTg < 1:1556989/66‑1 (MQ=255) ttGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTg < 1:1525544/66‑1 (MQ=255) ggcggcGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATTTCGa > 1:871863/1‑71 (MQ=255) ggcggcGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCg < 1:2562027/70‑1 (MQ=255) ggcggcGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCg < 1:2630038/70‑1 (MQ=255) gcggTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTAt < 1:2131832/71‑1 (MQ=255) cggTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGATGATGTCGATTATc < 1:3935792/71‑1 (MQ=255) cggTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGATGATGTCGATTATc < 1:3217989/71‑1 (MQ=255) cggTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGATGATGTCGATTATc < 1:3737020/71‑1 (MQ=255) cACTGACCAGTAAAGTCGCCATTATTATCCGTTCCAGCGATCCGCGTGATGATGTCGATTATCTGTTTgcg > 1:331150/1‑71 (MQ=255) cACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTgcg > 1:1574295/1‑71 (MQ=255) cACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTgcg > 1:3805851/1‑71 (MQ=255) cACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTgcg > 1:1238991/1‑71 (MQ=255) cACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGATGATGTCGATTATCTGTTTgcg > 1:916036/1‑71 (MQ=255) cACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGATGATGTCGATTATCTGTTTgcg > 1:1260924/1‑71 (MQ=255) cTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGa < 1:162413/56‑1 (MQ=255) cTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGa < 1:2964827/56‑1 (MQ=255) cTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGa < 1:3166086/56‑1 (MQ=255) tGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTg > 1:890070/1‑62 (MQ=255) tGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTg > 1:3465042/1‑62 (MQ=255) tAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCt < 1:1521541/40‑1 (MQ=255) tAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCt < 1:2139029/40‑1 (MQ=255) aaGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGt < 1:3571978/70‑1 (MQ=255) aaGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGATGATGTCGATTATCTGTTTGCGCAGGTAATCGtt > 1:3366486/1‑71 (MQ=255) aaGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGATGATGTCGATTATCTGTTTGCGCAGGTAATCGtt > 1:767836/1‑71 (MQ=255) gTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCt < 1:3753835/36‑1 (MQ=255) gTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCt < 1:4029550/36‑1 (MQ=255) tCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCAt > 1:128410/1‑71 (MQ=255) tCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTAACTGTTTGCGCAGGTAATCGTTCAt > 1:1675538/1‑71 (MQ=255) cGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCAt > 1:2375792/1‑70 (MQ=255) gCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGa < 1:3348520/41‑1 (MQ=255) cATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGc < 1:2243138/71‑1 (MQ=255) cATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGc < 1:4113740/71‑1 (MQ=255) cATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGc < 1:2156502/71‑1 (MQ=255) cATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGc < 1:17674/71‑1 (MQ=255) cATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGc < 1:493326/71‑1 (MQ=255) attattAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTa < 1:2174606/57‑1 (MQ=255) attattAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTa < 1:1427106/57‑1 (MQ=255) attattAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGc < 1:4113236/70‑1 (MQ=255) attattAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGc > 1:202426/1‑70 (MQ=255) ttattaGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTa < 1:707194/40‑1 (MQ=255) ttattaGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTa < 1:1988523/40‑1 (MQ=255) ttattaGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTa < 1:29839/40‑1 (MQ=255) ttattaGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTa < 1:701981/40‑1 (MQ=255) ttattaGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTa < 1:1244530/40‑1 (MQ=255) ttattaGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTa < 1:3916810/40‑1 (MQ=255) ttattaGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTa < 1:4095671/40‑1 (MQ=255) ttattaGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTa < 1:672147/40‑1 (MQ=255) tattaGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAAt > 1:3793048/1‑57 (MQ=255) tattaGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAAt > 1:4082154/1‑57 (MQ=255) tattaGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAAt > 1:1857778/1‑57 (MQ=255) attaGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGtt > 1:3312995/1‑44 (MQ=255) attaGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGtt > 1:3171849/1‑44 (MQ=255) attaGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGtt > 1:624931/1‑44 (MQ=255) gCCGTTCCAGCGATCCGCGTGATGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTc < 1:2280849/71‑1 (MQ=255) cGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGa < 1:2343483/71‑1 (MQ=255) cGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGa < 1:2434325/71‑1 (MQ=255) ttCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGt < 1:3286748/51‑1 (MQ=255) ttCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGt < 1:3525883/51‑1 (MQ=255) ttCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGt < 1:853930/51‑1 (MQ=255) ttCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGt < 1:1185196/51‑1 (MQ=255) ttCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCAt < 1:564898/55‑1 (MQ=255) ccAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGa > 1:1284586/1‑55 (MQ=255) ccAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGa > 1:2942900/1‑55 (MQ=255) ccAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGa > 1:366821/1‑55 (MQ=255) ccAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGa > 1:520648/1‑55 (MQ=255) ccAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATAc > 1:2667772/1‑70 (MQ=255) ccAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATAc > 1:1359107/1‑70 (MQ=255) ccAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATAc > 1:3784684/1‑70 (MQ=255) ccAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATAc > 1:3120224/1‑70 (MQ=255) ccAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATAc > 1:1228897/1‑70 (MQ=255) ccAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATAc > 1:2722436/1‑70 (MQ=255) ccAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATAc > 1:1103808/1‑70 (MQ=255) ccAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATAc > 1:2352911/1‑70 (MQ=255) ccAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATAc > 1:832473/1‑70 (MQ=255) cAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATACCa > 1:1055382/1‑71 (MQ=255) cAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATACCa > 1:3761747/1‑71 (MQ=255) cAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATACCa > 1:1007480/1‑71 (MQ=255) cAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATACCa > 1:1215797/1‑71 (MQ=255) cAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATACCa > 1:1643015/1‑71 (MQ=255) cAGCGATCAGCGTGATGATGTCGATTATCTGTTTGCGCAGGTAAt < 1:1629887/45‑1 (MQ=37) cAGCGATCAGCGTGATGATGTCGATTATCTGTTTGCGCAGGTAAt < 1:1805776/45‑1 (MQ=37) aGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTc < 1:2228346/49‑1 (MQ=255) aGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTc < 1:2079674/49‑1 (MQ=255) aGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTc < 1:3806404/49‑1 (MQ=255) cGATCCGCGTGATGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATAcc > 1:502257/1‑67 (MQ=255) cGATCCGCGTGATGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATAcaa > 1:1581502/1‑66 (MQ=255) cGATCCGCGTGATGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATACCa > 1:3631187/1‑68 (MQ=255) gATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAAc > 1:2500787/1‑55 (MQ=255) gATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACg > 1:3557071/1‑56 (MQ=255) gATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACg > 1:1298333/1‑56 (MQ=255) gATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACg > 1:370173/1‑56 (MQ=255) tGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATACCACGCCTaa > 1:2281652/1‑66 (MQ=255) tGCTGAAGTCGATTATCTGTTTGCGGAGGTAATCGTTCATGAGCAACGTGTCGATACCACGCCTaa > 1:942281/1‑66 (MQ=255) tGCAGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATACCACGCCTaa > 1:2584446/1‑66 (MQ=255) | ACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATACCACGCCTAACTGCGGC > minE/507356‑507495 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |