| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 581,876 | G→A | 27.2% | P638P (CCG→CCA) | clpA → | ATPase and specificity subunit of ClpA‑ClpP ATP‑dependent serine protease, chaperone activity |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 581,876 | 0 | G | A | 27.2% | 139.4 / 57.5 | 81 | P638P (CCG→CCA) | clpA | ATPase and specificity subunit of ClpA‑ClpP ATP‑dependent serine protease, chaperone activity |
| Reads supporting (aligned to +/- strand): ref base G (31/28); new base A (10/12); total (41/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.24e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.46e-01 | |||||||||||
TCCATTGGTCTTATCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCAACCGACGTGATCCATCAGGT > minE/581808‑581944 | tCCATTGGTCTTATCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCgg > 1:2364668/1‑70 (MQ=255)tCCATTGGTCTTATCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCgg > 1:423341/1‑70 (MQ=255) tCTTATCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCGGAATTCCGTa < 1:886140/71‑1 (MQ=255) tCTTATCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCGGAATTCCGTa < 1:3329778/71‑1 (MQ=255) tCTTATCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCGGAATTCCGTa < 1:1134426/71‑1 (MQ=255) tCTTATCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCGGAATTCCGTa < 1:2832762/71‑1 (MQ=255) ttATCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCGGAATTCCGTAAc > 1:3784521/1‑71 (MQ=255) ttATCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCGGAATTCCGTAAc > 1:2638102/1‑71 (MQ=255) ttATCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCGGAATTCCGTAAc > 1:1176213/1‑71 (MQ=255) tATCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCAGAATTCCGTAAcc > 1:2523944/1‑71 (MQ=255) tATCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCAGAATTCCGTAAcc > 1:2215012/1‑71 (MQ=255) tATCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCAGAATTCCGTAAcc > 1:4121645/1‑71 (MQ=255) tATCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCAGAATTCCGTAAcc > 1:1476621/1‑71 (MQ=255) tATCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCAGAATTCCGTAAcc > 1:841620/1‑71 (MQ=255) aTCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCGGAATTCCGTaa < 1:1323300/68‑1 (MQ=255) aTCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCGGAATTCCGTaa < 1:1597852/68‑1 (MQ=255) aTCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCGGAATTCCGTAACCg > 1:2403995/1‑71 (MQ=255) aTCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCGGAATTCCGTAACCg > 1:788334/1‑71 (MQ=255) aTCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCGGAATTCCGTAACCg > 1:2727127/1‑71 (MQ=255) aTCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCGGAATTCCGTAACCg > 1:271362/1‑71 (MQ=255) aTCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCGGAATTCCGTAACCg > 1:2306650/1‑71 (MQ=255) gATAACAGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGacaa < 1:2775379/71‑1 (MQ=255) gATAACAGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGacaa < 1:3684780/71‑1 (MQ=255) gATAACAGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGacaa < 1:1115385/71‑1 (MQ=255) tAACAGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGacaaca > 1:1372004/1‑71 (MQ=255) tAACAGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGacaaca > 1:2467830/1‑71 (MQ=255) aGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGACAACATTa > 1:870286/1‑70 (MQ=255) aGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCAGAATTCCGTAACCGTCTCGACAACAtt > 1:3039718/1‑69 (MQ=255) aGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCAGAATTCCGTAACCGTCTCGACAACATTa > 1:1283954/1‑70 (MQ=255) aGCACCGATCCGATGGAAGAGATCAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGACAACATTAt > 1:2509357/1‑71 (MQ=255) attcgatgGAAGAGATCAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGttt > 1:3934865/4‑71 (MQ=255) atgcgatgGAAGAGATCAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGttt > 1:309094/1‑71 (MQ=255) atgcgatgGAAGAGATCAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGtt > 1:1001809/1‑70 (MQ=255) tgcgatgGAAGAGATCAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTg < 1:3945602/71‑1 (MQ=255) gcgatgGAAGAGATCAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGa > 1:2576235/1‑71 (MQ=255) gcgatgGAAGAGATCAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGa > 1:2612753/1‑71 (MQ=255) gcgatgGAAGAGATCAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGa > 1:3594868/1‑71 (MQ=255) ggAAGAGATCAAGAAGATCTTTACACCGGAATTCCGTAAcgg > 1:1146878/1‑40 (MQ=255) gAAGAGATCAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGatcatc < 1:3879333/70‑1 (MQ=255) gAAGAGATCAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGatcatc < 1:3884206/70‑1 (MQ=255) gAAGAGATCAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGatcatc < 1:3107460/70‑1 (MQ=255) gaTCAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCaa > 1:3687188/1‑71 (MQ=255) gaTCAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCaa > 1:3973295/1‑71 (MQ=255) tCAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCAAcc < 1:1333867/71‑1 (MQ=255) tCAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCAAcc < 1:3265015/71‑1 (MQ=255) tCAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCAAcc < 1:1126108/71‑1 (MQ=255) cAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCAAcc > 1:2916861/1‑70 (MQ=255) cAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCAAcc > 1:2995894/1‑70 (MQ=255) cAAGAAGATCTTTACACCAGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGatca > 1:1831712/1‑60 (MQ=255) cAAGAAGATCTTTACACCAGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGatca > 1:3504180/1‑60 (MQ=255) cAAGAAGATCTTTACACCAGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGatca > 1:2039432/1‑60 (MQ=255) aagaagATCTTTTCACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCAACCg < 1:1322041/70‑1 (MQ=255) aagaagATCTTTACACCAGAATTCCGTAACCGTCTCGacaac < 1:2541953/42‑1 (MQ=255) aagaagATCTTTACACCAGAATTCCGTAACCGTCTCGacaac < 1:1047795/42‑1 (MQ=255) aagaagATCTTTACACCAGAATTCCGTAACCGTCTCGacaac < 1:2116873/42‑1 (MQ=255) aagaagATCTTTACACCAGAATTCCGTAACCGTCTCGacaac < 1:2666879/42‑1 (MQ=255) aagaagATCTTTACACCAGAATTCCGTAACCGTCTCGacaac < 1:3288200/42‑1 (MQ=255) aagaagATCTTTACACCAGAATTCCGTAACCGTCTCGacaac < 1:741881/42‑1 (MQ=255) aagaagATCTTTACACCAGAATTCCGTAACCGTCTCGacaac < 1:2952326/42‑1 (MQ=255) agaagaTCTTTACACCAGAATTCCGTAACCGTCTCGACAACAt < 1:3647648/43‑1 (MQ=255) agaagaTCTTTACACCAGAATTCCGTAACCGTCTCGACAACAt < 1:1707045/43‑1 (MQ=255) agaagaTCTTTACACCAGAATTCCGTAACCGTCTCGACAACAt < 1:281539/43‑1 (MQ=255) gaagaTCTTTACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCAACCGACg > 1:833878/1‑71 (MQ=255) gaagaTCTTTACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCAACCGACg > 1:1014673/1‑71 (MQ=255) gaagaTCTTTACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCAACCGACg > 1:1889555/1‑71 (MQ=255) gaagaTCTTTACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCAACCGACg > 1:2032407/1‑71 (MQ=255) gaagaTCTTTACACCAGAATTCCGTAACCGTCTCGactac < 1:540290/40‑1 (MQ=37) gaagaTCTTTACACCAGAATTCCGTAACCGTCTCGACAACAt < 1:3815530/42‑1 (MQ=255) tCTTTACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCAACCGACGTGAt < 1:1462229/70‑1 (MQ=255) tCTTTACACCGGAATTCCGTAACCGGCTCGACAACATTATCTGGTTTGATCATCTGTCAACCGACGTGAt < 1:3306131/70‑1 (MQ=255) cTTTACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCAACCGACGTGATc > 1:2890856/1‑70 (MQ=255) cTTTACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCAACCGACGTGATc > 1:776886/1‑70 (MQ=255) cTTTACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTCTCAACCGACGTGATc > 1:1075716/1‑70 (MQ=255) tACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCAACCGACGTGATc < 1:2925252/67‑1 (MQ=255) tACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCAACCGACGTGATc < 1:841616/67‑1 (MQ=255) tACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCAACCGACGTGATCCAt < 1:3586503/70‑1 (MQ=255) tACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCAACCGACGTGATCCAt < 1:209646/70‑1 (MQ=255) tACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCAACCGACGTGATCCAt > 1:295653/1‑70 (MQ=255) acacCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCAACCGACGTGATCCATc < 1:1732472/70‑1 (MQ=255) acacCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCAACCGACGTGATCCATc < 1:1083591/70‑1 (MQ=255) acacCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCAACCGACGTGATCCATc < 1:887180/70‑1 (MQ=255) ccGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCAACCGACGTGATCCATCAggt < 1:3982788/71‑1 (MQ=255) ccGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCAACCGACGTGATCCATCAggt < 1:3912194/71‑1 (MQ=255) | TCCATTGGTCTTATCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCGGAATTCCGTAACCGTCTCGACAACATTATCTGGTTTGATCATCTGTCAACCGACGTGATCCATCAGGT > minE/581808‑581944 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |