| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 597,552 | C→T | 27.1% | intergenic (+133/‑106) | serS → / → dmsA | seryl‑tRNA synthetase, also charges selenocysteinyl‑tRNA with serine/dimethyl sulfoxide reductase, anaerobic, subunit A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 597,552 | 0 | C | T | 27.1% | 100.8 / 40.7 | 59 | intergenic (+133/‑106) | serS/dmsA | seryl‑tRNA synthetase, also charges selenocysteinyl‑tRNA with serine/dimethyl sulfoxide reductase, anaerobic, subunit A |
| Reads supporting (aligned to +/- strand): ref base C (21/22); new base T (8/8); total (29/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.93e-01 | |||||||||||
CAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGTTTGT > minE/597485‑597619 | cAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTTaaa > 1:3143179/1‑71 (MQ=255)cAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTTaaa > 1:2552731/1‑71 (MQ=255)cAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTTaaa > 1:193431/1‑71 (MQ=255) ttACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTTAAATTATTc < 1:3813080/71‑1 (MQ=255) ttACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTTAAATTATTc < 1:2285713/71‑1 (MQ=255) ttACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTc < 1:3809259/71‑1 (MQ=255) ttACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTc < 1:1330307/71‑1 (MQ=255) ctcACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTTAAATTATTCGATGta > 1:2225289/1‑71 (MQ=255) ctcACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGta > 1:3448410/1‑71 (MQ=255) ctcACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGta < 1:749427/71‑1 (MQ=255) ctcACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGta < 1:4113738/71‑1 (MQ=255) ctcACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGta < 1:3459538/71‑1 (MQ=255) tACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTTAAATTATTCGATGTATACAAg > 1:2212598/1‑71 (MQ=255) cGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTTAAATTATTCGATGTATACAAGCCta < 1:278580/71‑1 (MQ=255) cGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTTAAATTATTCGATGTATACAAGCCta < 1:128965/71‑1 (MQ=255) gTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTc > 1:3073716/1‑54 (MQ=255) gTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCta > 1:2953207/1‑70 (MQ=255) gTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCta > 1:1617581/1‑70 (MQ=255) gTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCta > 1:4044263/1‑70 (MQ=255) tAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCtata < 1:514005/71‑1 (MQ=255) tAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCtata < 1:904214/71‑1 (MQ=255) ctactTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGc > 1:3014599/1‑70 (MQ=255) actTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGaa > 1:1344032/1‑71 (MQ=255) actTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGa > 1:492416/1‑70 (MQ=255) ctTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAAc < 1:1038769/71‑1 (MQ=255) tttCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGta < 1:3748726/50‑1 (MQ=255) tCGAGTGAAAATCTACCTATCTCTTTGATTTTTAAATTATTCGATGTATACAAGCCTATATAGCGAAc < 1:1289952/68‑1 (MQ=255) tGAAAATCTACCTATCTCTTTGATTTTTAAATTATTCGATGta < 1:2526148/43‑1 (MQ=255) tGAAAATCTACCTATCTCTTTGATTTTTAAATTATTCGATGta < 1:750432/43‑1 (MQ=255) tGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATg < 1:2434760/41‑1 (MQ=255) tGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATg < 1:26185/41‑1 (MQ=255) tGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATg < 1:4098921/41‑1 (MQ=255) tGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATg < 1:3088674/41‑1 (MQ=255) tGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATg < 1:1112977/41‑1 (MQ=255) aaaaTCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGa > 1:1257684/1‑70 (MQ=255) aaaTCTACCTATCTCTTTGATTTTTAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGaaa > 1:221203/1‑71 (MQ=255) tACCTATCTCTTTGATTTTCAAATTATTCGATGta < 1:1043706/35‑1 (MQ=255) tctcTTTGATTTTTAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTacac < 1:4010155/69‑1 (MQ=255) tctTTGATTTTTAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAaataa > 1:1413646/1‑61 (MQ=255) tctTTGATTTTTAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATa > 1:405409/1‑71 (MQ=255) tctTTGATTTTCAAATTATTCGATGTATACAAGCCtata > 1:2761522/1‑39 (MQ=255) tctTTGATTTTCAAATTATTCGATGTATACAAGCCtata > 1:3724853/1‑39 (MQ=255) tctTTGATTTTCAAATTATTCGATGTATACAAGCCtata > 1:1306150/1‑39 (MQ=255) tctTTGATTTTCAAATTATTCGATGTATACAAGCCtata > 1:1869240/1‑39 (MQ=255) tctTTGATTTTCAAATTATTCGATGTATACAAGCCtata > 1:1944606/1‑39 (MQ=255) tGATTTTCAAATTATTCGATGTATACAAGCCtata > 1:248540/1‑35 (MQ=255) gATTTTCAAATTATTCGATGTATACAAGCCTATATAGTGAACTGCTATAGAAATAATTACACAATACGGtt > 1:817532/1‑71 (MQ=255) gATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGtt > 1:1156192/1‑71 (MQ=255) gATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGtt > 1:872371/1‑71 (MQ=255) gATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGtt > 1:17852/1‑71 (MQ=255) gATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGtt > 1:3712133/1‑71 (MQ=255) gATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGtt > 1:3126040/1‑71 (MQ=255) ttCAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGTTTGt < 1:388500/70‑1 (MQ=255) ttCAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGTTTGt < 1:2302970/70‑1 (MQ=255) ttCAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGTTTGt < 1:1674863/70‑1 (MQ=255) ttCAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGTTTGt < 1:1638214/70‑1 (MQ=255) ttCAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGTTTGt < 1:2507193/70‑1 (MQ=255) ttCAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGTTTGt < 1:3219015/70‑1 (MQ=255) tCAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGTTTGt < 1:3773617/69‑1 (MQ=255) | CAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGTTTGT > minE/597485‑597619 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |