| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 623,375 | C→T | 34.0% | T19I (ACC→ATC) | msbA → | fused lipid transporter subunits and membrane component and ATP‑binding component of ABC superfamily |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 623,375 | 0 | C | T | 34.0% | 60.6 / 48.6 | 53 | T19I (ACC→ATC) | msbA | fused lipid transporter subunits and membrane component and ATP‑binding component of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base C (25/10); new base T (7/11); total (32/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.70e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.91e-01 | |||||||||||
TGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTAATCCTCAACGCAGCCAGCGATAC > minE/623309‑623444 | tGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCAtt > 1:3295314/1‑71 (MQ=255) ttttGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGcc > 1:65021/1‑71 (MQ=255) ttttGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGcc > 1:2205538/1‑71 (MQ=255) tGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCa > 1:1051990/1‑69 (MQ=255) tGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCa > 1:1207181/1‑69 (MQ=255) tGCATAACGACAAAGATCTCTCTACGTGGCAGAAATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCa > 1:3667909/1‑69 (MQ=255) cATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAATCATTGCGCCTTTCAAAGc < 1:1829105/71‑1 (MQ=255) aCGATAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAATCATTGCGCCTTTCAAAGCGGGt > 1:1324718/1‑71 (MQ=255) aCGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAATCATTGCGCCTTTCAAAGCGGGt > 1:3067776/1‑71 (MQ=255) aCGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAATCATTGCGCCTTTCAAAGCGGGt > 1:3641639/1‑71 (MQ=255) aCGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAATCATTGCGCCTTTCAAAGCGGGt > 1:2345073/1‑71 (MQ=255) aCGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAATCATTGCGCCTTTCAAAGCGGGt > 1:1482690/1‑71 (MQ=255) aCGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCAt > 1:1626316/1‑52 (MQ=255) aCGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCaa < 1:1789794/64‑1 (MQ=255) aCGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCaa < 1:3170154/64‑1 (MQ=255) aCGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGt > 1:736708/1‑71 (MQ=255) aCGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGt > 1:3352112/1‑71 (MQ=255) aCGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGt > 1:3433670/1‑71 (MQ=255) aCAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTg < 1:267851/71‑1 (MQ=255) aCAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTg < 1:2991263/71‑1 (MQ=255) cAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTg < 1:2074722/70‑1 (MQ=255) aaaGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAATCATTGCGCCTTTCAAAGCGGGTCTGAt > 1:3516795/1‑71 (MQ=255) aaaGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGAt > 1:2370310/1‑71 (MQ=255) cGTGGCAGACATTCCGCCGACTGTGGCCAATCATTGCGCCTTTCAAAGCGGGTCTGATCGTggcgggcg > 1:3920280/1‑69 (MQ=255) cGTGGCAGACATTCCGCCGACTGTGGCCAATCATTGCGCCTTTCAAAGCGGGTCTGATCGTggcgggcg < 1:3610841/69‑1 (MQ=255) gCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGt > 1:697069/1‑71 (MQ=255) cAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGGGGGTCTGATCGTGGCGGGCGTAGCGtt < 1:647461/71‑1 (MQ=255) cAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGtt < 1:3942862/71‑1 (MQ=255) cAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGtt < 1:2053666/71‑1 (MQ=255) gACATTCCGCCGACTGTGGCCAATCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTaa < 1:1940094/71‑1 (MQ=255) aCATTCCGCCGACTGTGGCCAATCATTGCGCCTTTCa < 1:172855/37‑1 (MQ=255) aTTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCgg > 1:3800968/1‑41 (MQ=255) aTTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTAATcc > 1:1959516/1‑71 (MQ=255) aTTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTAATcc > 1:1095769/1‑71 (MQ=255) ccgccgACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTgg > 1:1051118/1‑50 (MQ=255) ccgccgACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTgg > 1:519143/1‑50 (MQ=255) ccgccgACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTAATCCTc > 1:3559908/1‑70 (MQ=255) ccgccgACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTAATCCTc > 1:582054/1‑70 (MQ=255) cgccgACTGTGGCCAATCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTAATCCTCaa < 1:3978728/71‑1 (MQ=255) aCTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTg < 1:2155493/37‑1 (MQ=255) tgtgGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTAATCCTCAACGcagc > 1:3281181/1‑70 (MQ=255) ccAATCATTGCGCCTTTCAAAGCGGTTCTGATCGTGGCGGGCGTAGCGTTAATCCTc < 1:3499282/57‑1 (MQ=255) ccAATCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTAATCCTc < 1:888139/57‑1 (MQ=255) ccAATCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTAATCCTc < 1:331259/57‑1 (MQ=255) ccAATCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTAATCCTc < 1:3690694/57‑1 (MQ=255) ccAATCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTAATCCTc < 1:1341624/57‑1 (MQ=255) ccAATCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTAATCCTc < 1:3040820/57‑1 (MQ=255) cAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTAATCCTCAACGCAGCCAGCGAt < 1:2326224/71‑1 (MQ=255) aaCCATTGCGCCTTTCAAAGCGGGTCTGATCGTggc > 1:3981305/1‑36 (MQ=255) aaCCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTAATCCTCAACGCAGCCAGCGATa > 1:462861/1‑71 (MQ=255) aaCCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTAATCCTCAACGCAGCCAGCGATa > 1:3786231/1‑71 (MQ=255) aCCATTGCGCCTTTCAAAGCGGGTCTGATCGTggcg > 1:2438095/1‑36 (MQ=255) aCCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTAATCCTCAACGCAGCCAGCGATAc > 1:128272/1‑71 (MQ=255) ccATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTAATCCTCaa > 1:1689574/1‑55 (MQ=255) ccATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTAATCCTCaa > 1:2200208/1‑55 (MQ=255) | TGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTAATCCTCAACGCAGCCAGCGATAC > minE/623309‑623444 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |