| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 656,044 | T→C | 15.7% | F44L (TTC→CTC) | ycbS → | predicted outer membrane usher protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 656,044 | 0 | T | C | 15.7% | 172.9 / 23.8 | 70 | F44L (TTC→CTC) | ycbS | predicted outer membrane usher protein |
| Reads supporting (aligned to +/- strand): ref base T (32/27); new base C (6/5); total (38/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGGTGGGCTGGTGGTGTTTGTGTCGGCAGCGTTCAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACAAAAAATAAC > minE/655974‑656105 | tggtggGCTGGTGGTGTTTGTGTCGGCAGCGTTCAATGCACAAGCTGAAACCTGGTTCGATCCTGCttttt > 1:452138/1‑71 (MQ=255) ggtggGCTGGTGGTGTTTGTGTCGGCAGCGTTCAATGCACAAGCTGAAACCTGGTTCGATCCTGCtttttt > 1:1394455/1‑71 (MQ=255) ggCTGGTGGTGTTTGTGTCGGCAGCGTTCAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCaaa < 1:2635972/71‑1 (MQ=255) ggCTGGTGGTGTTTGTGTCGGCAGCGTTCAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCaaa < 1:1232245/71‑1 (MQ=255) gCTGGTGGTGTTTGTGTCGGCAGCGTTCAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAg < 1:1010234/71‑1 (MQ=255) gtggtgTTTGTGTCGGCAGCGTTCAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTCTCAAAgatga < 1:3987055/71‑1 (MQ=255) tggtgTTTGTGTCGGCAGCGTTCAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTCTCAAAgatga < 1:3991792/70‑1 (MQ=255) tgtgTCGGCAGCGTTCAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTAAAAgatga > 1:1611878/1‑63 (MQ=255) tgtgTCGGCAGCGTTCAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTCTCAAAGATGATCCCTCaa > 1:3732593/1‑71 (MQ=255) tgtCGGCAGCGTTCAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAgat > 1:2662511/1‑59 (MQ=255) aGCGTTCAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCg < 1:3450474/71‑1 (MQ=255) aGCGTTCAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCg < 1:1808359/71‑1 (MQ=255) gCGTTCAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGa < 1:147080/71‑1 (MQ=255) gCGTTCAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGa < 1:353456/71‑1 (MQ=255) gTTCAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAgatgat > 1:2642447/1‑52 (MQ=255) gTTCAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAgatgat > 1:766648/1‑52 (MQ=255) gTTCAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTAAAAg > 1:658869/1‑47 (MQ=255) ttCAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAgatga < 1:758641/50‑1 (MQ=255) ttCAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAgatga < 1:2601405/50‑1 (MQ=255) ttCAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAgatga < 1:2547434/50‑1 (MQ=255) ttCAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGAttt > 1:199915/1‑71 (MQ=255) ttCAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGAttt > 1:531077/1‑71 (MQ=255) tCAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTg > 1:3112677/1‑71 (MQ=255) tCAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTg > 1:1079429/1‑71 (MQ=255) tCAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTg > 1:555768/1‑71 (MQ=255) cAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCaaaca > 1:381690/1‑43 (MQ=255) tGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAAtg < 1:3907903/55‑1 (MQ=255) tGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAAtg < 1:2720167/55‑1 (MQ=255) tGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAAtg < 1:1781869/55‑1 (MQ=255) tGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAAtg < 1:3325283/55‑1 (MQ=255) tGCACAAGCTGAAACCTGGTTAGATCCTGCTTTTTTCAAAGATGATCCCTCAAtg < 1:2464392/55‑1 (MQ=255) gCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCg > 1:1785644/1‑71 (MQ=255) cacaAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCaa > 1:3853179/1‑51 (MQ=255) cacaAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGt > 1:4076341/1‑71 (MQ=255) cacaAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGt > 1:3211061/1‑71 (MQ=255) cacaAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGt > 1:2629271/1‑71 (MQ=255) cacaAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGt > 1:2492239/1‑71 (MQ=255) caAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAgatg < 1:3357854/40‑1 (MQ=255) caAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAga > 1:3248628/1‑38 (MQ=255) caAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAga > 1:2900254/1‑38 (MQ=255) caAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAga > 1:359211/1‑38 (MQ=255) aaaCCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCg > 1:2618825/1‑51 (MQ=255) aaaCCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCg > 1:319940/1‑51 (MQ=255) aaaCCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCg > 1:3017924/1‑51 (MQ=255) aaaCCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCg > 1:2096777/1‑51 (MQ=255) aCCTGGTTCGATCCTGCTTTTCTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAg > 1:2667469/1‑70 (MQ=255) aCCTGGTTCGATCCTGCTTTTCTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAg > 1:641802/1‑70 (MQ=255) gTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACaaa < 1:3138061/71‑1 (MQ=255) gTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACaaa < 1:1164064/71‑1 (MQ=255) ttCGATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACaaa > 1:1172683/1‑70 (MQ=255) ttCGATCCTGCTTTTCTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACaa > 1:583104/1‑69 (MQ=255) gATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACAAAAAAt < 1:2566370/71‑1 (MQ=255) gATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACAAAAAAt < 1:985610/71‑1 (MQ=255) gATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACAAAAAAt < 1:3244796/71‑1 (MQ=255) gATCCTGCTTTTCTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACAAAAAAt < 1:43502/71‑1 (MQ=255) gATCCTGCTTTTCTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACAAAAAAt < 1:86235/71‑1 (MQ=255) aTCCTGCTTTTCTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACAAAAAAt < 1:3369900/70‑1 (MQ=255) tCCTGCTTTTCTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACAAAAAATaa > 1:4011646/1‑71 (MQ=255) tCCTGCTTTTCTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACAAAAAATaa > 1:2915307/1‑71 (MQ=255) ccTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGAtt > 1:1863833/1‑40 (MQ=255) ccTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACaaaaaa > 1:2358198/1‑67 (MQ=255) ccTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACaaaaaa > 1:1633171/1‑67 (MQ=255) cTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGAttt < 1:3643278/40‑1 (MQ=255) cTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGAttt < 1:841986/40‑1 (MQ=255) cTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGAttt < 1:834533/40‑1 (MQ=255) cTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGAttt < 1:629587/40‑1 (MQ=255) cTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACAAAAAATAAc < 1:3247018/70‑1 (MQ=255) cTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACAAAAAATAAc < 1:2997128/70‑1 (MQ=255) cTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACaaaaa > 1:1178994/1‑62 (MQ=255) cTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACaaaaa > 1:3436785/1‑62 (MQ=255) cTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACaaaaa > 1:521555/1‑62 (MQ=255) cTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACaaaaa > 1:3869410/1‑62 (MQ=255) | TGGTGGGCTGGTGGTGTTTGTGTCGGCAGCGTTCAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACAAAAAATAAC > minE/655974‑656105 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |