| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 696,779 | C→T | 32.0% | P262L (CCC→CTC) | appB → | cytochrome bd‑II oxidase, subunit II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 696,779 | 0 | C | T | 32.0% | 64.5 / 41.8 | 50 | P262L (CCC→CTC) | appB | cytochrome bd‑II oxidase, subunit II |
| Reads supporting (aligned to +/- strand): ref base C (21/13); new base T (4/12); total (25/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.22e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01 | |||||||||||
CAATCCGTTAATGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGA > minE/696714‑696838 | cAATCCGTTAATGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCTCGttt > 1:534274/1‑71 (MQ=255)cAATCCGTTAATGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGttt > 1:3736118/1‑71 (MQ=255) aaTGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATc < 1:1072101/70‑1 (MQ=255) aaTGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATc < 1:2951636/70‑1 (MQ=255) aaTGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCt > 1:1902396/1‑71 (MQ=255) ggtggCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCTCGTTTTGTGGATCTTCCCgctgc > 1:803713/1‑71 (MQ=255) ggtggCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCTCGTTTTGTGGATCTTCCCgctgc > 1:647421/1‑71 (MQ=255) ggtggCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCgctgc > 1:3813715/1‑71 (MQ=255) ggtggCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCAgctgc > 1:3311174/1‑71 (MQ=255) tggCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCTCGTTTTGTGGATCTTCCCGctgctg < 1:3131834/71‑1 (MQ=255) tggCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCTCGTTTTGTGGATCTTCCCGctgctg < 1:4068272/71‑1 (MQ=255) aGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGAt > 1:2897874/1‑71 (MQ=255) gTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCTCGTTTTGTgg < 1:336652/51‑1 (MQ=255) gTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCTCGTTTTGTgg < 1:3053666/51‑1 (MQ=255) gTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCTCGTTTTGTgg < 1:2440492/51‑1 (MQ=255) gCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGAttct > 1:3340159/1‑71 (MQ=255) gCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGAttct > 1:3142521/1‑71 (MQ=255) gCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGAttct > 1:3152997/1‑71 (MQ=255) cTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCTCCCGTTTTGTGGATCTTCCCGCTGCTGGGAttct < 1:914322/70‑1 (MQ=255) cTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGAttctt > 1:1386705/1‑71 (MQ=255) cTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGAttctt > 1:451505/1‑71 (MQ=255) ccTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCgctgc > 1:2537145/1‑58 (MQ=255) ccTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTg > 1:2500965/1‑71 (MQ=255) ccTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTg > 1:1903010/1‑71 (MQ=255) ccTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTg > 1:1312747/1‑71 (MQ=255) tGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTtg > 1:1146965/1‑40 (MQ=255) ggTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGcc > 1:50426/1‑70 (MQ=255) gTGCCTGGATGAATAATTTTGTCGAGTCGCTCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGcc < 1:3163249/69‑1 (MQ=255) gTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGccc < 1:330891/70‑1 (MQ=255) gTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGccc < 1:3733673/70‑1 (MQ=255) gTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGccc < 1:2294894/70‑1 (MQ=255) gTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCa > 1:2723198/1‑71 (MQ=255) tGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGccc < 1:3150156/69‑1 (MQ=255) tGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCAt < 1:2673520/71‑1 (MQ=255) ccTGGATGAATAATTTTGTCGAGTCGCTCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTg < 1:385774/71‑1 (MQ=255) ccTGGATGAATAATTTTGTCGAGTCGCTCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTg < 1:747000/71‑1 (MQ=255) tGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTgg > 1:2153649/1‑36 (MQ=255) tGGATGAATAATTTTGTCGAGTCCCCCGTTTTGTgg > 1:3966245/1‑36 (MQ=255) gATGAATAATTTTGTCGAGTCGCTCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCt < 1:2472040/69‑1 (MQ=255) gATGAATAATTTTGTCGAGTCGCTCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCt < 1:1472773/69‑1 (MQ=255) aataatTTTGTCGAGTCGCTCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGt < 1:3504232/71‑1 (MQ=255) aataatTTTGTCGAGTCGCTCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGt < 1:475390/71‑1 (MQ=255) aataatTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGt < 1:1625947/71‑1 (MQ=255) taatTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCt < 1:3172848/63‑1 (MQ=255) atTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATg > 1:547371/1‑71 (MQ=255) ttGTCGAGTCGCTCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGc > 1:2141396/1‑57 (MQ=255) gTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGa < 1:12716/70‑1 (MQ=255) gAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGAttcttc > 1:1420662/1‑42 (MQ=255) cGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGt < 1:3790078/55‑1 (MQ=255) cGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGt < 1:1744993/55‑1 (MQ=255) | CAATCCGTTAATGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGA > minE/696714‑696838 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |