| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 843,375 | A→G | 8.9% | A80A (GCA→GCG) | galU → | glucose‑1‑phosphate uridylyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 843,375 | 0 | A | G | 8.9% | 223.5 / 11.8 | 79 | A80A (GCA→GCG) | galU | glucose‑1‑phosphate uridylyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (19/53); new base G (4/3); total (23/56) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.85e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01 | |||||||||||
GTGCTGGTTACACACTCATCTAAAAACTCTATTGAAAACCACTTTGATACCAGTTTTGAACTGGAAGCAATGCTGGAAAAACGTGTAAAACGTCAACTGCTTGATGAAGTGCAGTCTATTTGTCCACCGCA > minE/843307‑843437 | gtgCTGGTTACACACTCATCTAAAAACTCTATTGAAAACCACTTTGATACCAGTTTTGAACTGGAAGCAAt > 1:431206/1‑71 (MQ=255) tACACACTCATCTAAAAACTCTATTGAAAACCACTTTGATACCAGTTTTGAACTGGAAGCAATGCTGGaa > 1:3451898/1‑70 (MQ=255) acacacTCATCTAAAAACTCTATTGAAAACCACTTTGATACCAGTTTTGAACTGGAAGCAATGCTGGaaa < 1:2354200/70‑1 (MQ=255) acacacTCATCTAAAAACTCTATTGAAAACCACTTTGATACCAGTTTTGAACTGGAAGCAATGCTGGaaa < 1:3392178/70‑1 (MQ=255) acacacTCATCTAAAAACTCTATTGAAAACCACTTTGATACCAGTTTTGAACTGGAACCAATGCTGGaaa < 1:3730025/70‑1 (MQ=255) cacacTCATCTAAAAACTCTATTGAAAACCACTTTGATACCAGTTTTGAACTGGAAGCAATGCTGGaaaa < 1:1546445/70‑1 (MQ=255) cacacTCATCTAAAAACTCTATTGAAAACCACTTTGATACCAGTTTTGAACTGGAAGCAATGCTGGaaaa < 1:2621307/70‑1 (MQ=255) tCATCTAAAAACTCTATTGAAAACCACTTTGATACCAGTTTTGAACTGGAAGCAATGCTGGAAAAACgtg > 1:70131/1‑70 (MQ=255) cATCTAAAAACTCTATTGAAAACCACTTTGATACCAGTTTTGAACTGGAAGCAATGCTGGAAAAACgtgt > 1:1898037/1‑70 (MQ=255) cATCTAAAAACTCTATTGAAAACCACTTTGATACCAGTTTTGAACTGGAAGCAATGCTGGAAAAACgtgt > 1:1584228/1‑70 (MQ=255) cATCTAAAAACTCTATTGAAAACCACTTTGATACCAGTTTTGAACTGGAAGCAATGCTGGAAAAACgtgt > 1:3584288/1‑70 (MQ=255) cATCTAAAAACTCTATTGAAAACCACTTTGATACCAGTTTTGAACTGGAAGCAATGCTGGAAAAACgtgt > 1:297797/1‑70 (MQ=255) aTCTAAAAACTCTATTGAAAACCACTTTGATACCAGTTTTGAACTGGAAGCAATGCTGGAAAAACGTGTaa < 1:3664131/71‑1 (MQ=255) aTCTAAAAACTCTATTGAAAACCACTTTGATACCAGTTTTGAACTGGAAGCAATGCTGGAAAAACGTGTaa < 1:83389/71‑1 (MQ=255) tCTAAAAACTCTATTGAAAACCACTTTGATACCAGTTTTGAACTGGAAGCAATGCTTGaaa < 1:2252033/61‑1 (MQ=255) tCTAAAAACTCTATTGAAAACCACTTTGATACCAGTTTTGAACTGGAAGCAATGCTGGaaa < 1:247140/61‑1 (MQ=255) tCTAAAAACTCTATTGAAAACCACTTTGATACCAGTTTTGAACTGGAAGCAATGCTGGaaa < 1:3099747/61‑1 (MQ=255) tCTAAAAACTCTATTGAAAACCACTTTGATACCAGTTTTGAACTGGAAGCAATGCTGGaaa < 1:2349497/61‑1 (MQ=255) cTAAAAACTCTATTGAAAACCACTTTGATACCAGTTTTGAACTGGAAGCAATGCTGGaaa < 1:720358/60‑1 (MQ=255) tAAAAACTCTATTGAAAACCACTTTGATACCAGTTTTGAACTGGAAGCAATGCTGGAAAAACGTGTAAAAc > 1:3996312/1‑71 (MQ=255) tAAAAACTCTATTGAAAACCACTTTGATACCAGTTTTGAACTGGAAGCAATGCTGGAAAAACGTGTAAAAc > 1:465419/1‑71 (MQ=255) tAAAAACTCTATTGAAAACCACTTTGATACCAGTTTTGAACTGGAAGCAATGCTGGAAAAACGTGTAAAAc > 1:3665563/1‑71 (MQ=255) aaaaCTCTATTGAAAACCACTTTGATACCAGTTTTGAACTGGAAGCAATGCTGGAAAAACGTGTAAAACGt > 1:1538186/1‑71 (MQ=255) aaaaCTCTATTGAAAACCACTTTGATACCAGTTTTGAACTGGAAGCAATGCTGGAAAAACGTGTAAAACGt > 1:2393483/1‑71 (MQ=255) aCTCTATTGAAAACCACTTTGATACCAGTTTTGAACTGGAAGCAATGCTGGAAAAAc > 1:303252/1‑57 (MQ=255) tctATTGAAAACCACTTTGATACCAGTTTTGAACTGGAAGCaa > 1:3173670/1‑43 (MQ=255) ctATTGAAAACCACTTTGATACCAGTTTTGAACTGGAAGCGATGCTGGAAAAACGTGTAAAACGTCAACTg > 1:4107494/1‑71 (MQ=255) ctATTGAAAACCACTTTGATACCAGTTTTGAACTGGAAGCGATGCTGGAAAAACGTGTAAAACGTCAACTg > 1:1106985/1‑71 (MQ=255) ctATTGAAAACCACTTTGATACCAGTTTTGAACTGGAAGCGATGCTGGAAAAACGTGTAAAACGTCAACTg > 1:3581307/1‑71 (MQ=255) ctATTGAAAACCACTTTGATACCAGTTTTGAACTGGAAGCGATGCTGGAAAAACGTGTAAAACGTCAACTg > 1:3450354/1‑71 (MQ=255) tATTGAAAACCACTTTGATACCAGTTTTGAACTGGAAGCAATGCTGGaaa < 1:2420326/50‑1 (MQ=255) tATTGAAAACCACTTTGATACCAGTTTTGAACTGGAAGCAATGCTGGaaa < 1:1326835/50‑1 (MQ=255) tGAAAACCACTTTGATACCAGTTTTGAACTGGAAGCAATGCTGGAAAAAGGTGTAAAACGTCAACTGCtt > 1:3281755/1‑70 (MQ=255) tGAAAACCACTTTGATACCAGTTTTGAACTGGAAGCAATGCTGGAAAAACGTGTAAAACGTCAACTGCtt > 1:4106329/1‑70 (MQ=255) gAAAACCACTTTGATACCAGTTTTGAACTGGAAGAAATGCTGGAAAAACGTGTAAAACGTCAACTGCTtga > 1:3422388/1‑71 (MQ=255) aaaCCACTTTGATACCAGTTTTGAACTGGAAGCGATGCTGGAAAAACGTGTAAAACGTCAACTGCTtgatg < 1:621677/71‑1 (MQ=255) aaaCCACTTTGATACCAGTTTTGAACTGGAAGCAATGCTGGAACAACGTGTAAAACGTCAACTGCTtgatg < 1:1101615/71‑1 (MQ=255) aaaCCACTTTGATACCAGTTTTGAACTGGAAGCAATGCTGGAAAAACGTGTAAAACGTCAACTGCTtgatg < 1:2781185/71‑1 (MQ=255) aaaCCACTTTGATACCAGTTTTGAACTGGAAGCAATGCTGGAAAAACGTGTAAAACGTCAACTGCTtgatg < 1:4100367/71‑1 (MQ=255) aaaCCACTTTGATACCAGTTTTGAACTGGAAGCAATGCTGGAAAAACGTGTAAAACGTCAACTGCTtgatg < 1:2403270/71‑1 (MQ=255) aaaCCACTTTGATACCAGTTTTGAACTGGAAGCAATGCTGGAAAAACGTGTAAAACGTCAACTGCTtgatg < 1:1589393/71‑1 (MQ=255) aaaCCACTTTGATACCAGTTTTGAACTGGAAGCAATGCTGGAAAAACGTGTAAAACGTCAACTGCTtgatg < 1:1106036/71‑1 (MQ=255) aaCCACTTTGATACCAGTTTTGAACTGGAAGCAATGCTGGAAAAACGTGTAAAACGTCAACTGCTtgatga < 1:971783/71‑1 (MQ=255) aaCCACTTTGATACCAGTTTTGAACTGGAAGCAATGCTGGAAAAACGTGTAAAACGTCAACTGCTtgatga < 1:1364330/71‑1 (MQ=255) aCCACTTTGATACCAGTTTTGAACTGGAAGCAATGCTGGAAAAACGTGTAAAACGTCAACTGCTtgatga < 1:843633/70‑1 (MQ=255) aCTTTGATACCAGTTTTGAACTGGAAGCAATGCTGGAAAAACGTGTAAAACGTCAACTGCTTGATGAAGTg < 1:731133/71‑1 (MQ=255) aCTTTGATACCAGTTTTGAACTGGAAGCAATGCTGGAAAAACGTGTAAAACGTCAACTGCTTGATGAAGTg < 1:1401556/71‑1 (MQ=255) cTTTGATACCAGTTTTGAACTGGAAGCAATGCTGGAAAAACGTGTAAAACGTCAACTGCTtgatga < 1:3513569/66‑1 (MQ=255) cTTTGATACCAGTTTTGAACTGGAAGCAATGCTGGAAAAACGTGTAAAACGTCAACTGCTTGATGAAGTg < 1:2967145/70‑1 (MQ=255) tACCAGTTTTGAACTGGAAGCGATGCTGGAAAAACGTGTAAATCGTCAACTGCTTGATGAAGTGCAGTCTa < 1:3836433/71‑1 (MQ=255) tACCAGTTTTGAACTGGAAGCGATGCTGGAAAAACGTGTAAAACGTCAACTGCTTGATGAAGTGCAGTCTa < 1:342370/71‑1 (MQ=255) ccAGTTTTGAACTGGAAGCAATGCTGGAAAAACGTGTAAAACGTCAACTGCTTGATGAAGTGCAGTCTAt < 1:3017526/70‑1 (MQ=255) ccAGTTTTGAACTGGAAGCAATGCTGGAAAAACGTGTAAAACGTCAACTGCTTGATGAAGTGCAGTCTAt < 1:791292/70‑1 (MQ=255) ccAGTTTTGAACTGGAAGCAATGCTGGAAAAACGTGTAAAACGTCAACTGCTTGATGAAGTGCAGTCTAt < 1:2653594/70‑1 (MQ=255) ccAGTTTTGAACTGGAAGCAATGCTGGAAAAACGTGTAAAACGTCAACTGCTTGATGAAGTGCAGTCTAt < 1:2840771/70‑1 (MQ=255) aGTTTTGAACTGGAAGCAATGCTGGAAAAACGTGTAAAACGTCAACTGCTTGATGAAGTGCAGTCTATTTg < 1:1447278/71‑1 (MQ=255) aGTTTTGAACTGGAAGCAATGCTGGAAAAACGTGTAAAACGTCAACTGCTTGATGAAGTGCAGTCTATTTg < 1:2686272/71‑1 (MQ=255) aGTTTTGAACTGGAAGCAATGCTGGAAAAACGTGTAAAACGTCAACTGCTTGATGAAGTGCAGTCTATTTg < 1:3314617/71‑1 (MQ=255) aGTTTTGAACTGGAAGCAATGCTGGAAAAACGTGTAAAACGTCAACTGCTTGATGAAGTGCAGTCTATTTg < 1:2991011/71‑1 (MQ=255) ttttGAACTGGAAGCAATGCTGGAAAAACGTGTAAAACGTCAACTGCTTGATGAAGTGCAGTCTATTTGTc < 1:2686510/71‑1 (MQ=255) ttGAACTGGAAGCAATGCTGGAAAAACGTGTAAAACGTCAACTGCTTGATGAAGTGCAGTCTATTTGTCCa > 1:2851339/1‑71 (MQ=255) ttGAACTGGAAGCAATGCTGGAAAAACGTGTAAAACGTCAACTGCTTGATGAAGTGCAGTCTATTTGTCCa > 1:2564792/1‑71 (MQ=255) ttGAACTGGAAGCAATGCTGGAAAAACGTGTAAAACGTCAACTGCTTGATGAAGTGCAGTCTATTTGTCCa > 1:336650/1‑71 (MQ=255) aCTGGAAGCAATGCTGGAAAAACGTGTAAAACGTCAACTGCTTGATGAAGTGCAGTCTATTTGTCCACCGc < 1:3167805/71‑1 (MQ=255) aCTGGAAGCAATGCTGGAAAAACGTGTAAAACGTCAACTGCTTGATGAAGTGCAGTCTATTTGTCCACCGc < 1:2148758/71‑1 (MQ=255) cTGGAAGCAATGCTGGAAAAACGTGTAAAACGTCAACTGCTtgatg < 1:1242984/46‑1 (MQ=255) cTGGAAGCAATGCTGGAAAAACGTGTAAAACGTCAACTGCTtgatg < 1:3150843/46‑1 (MQ=255) cTGGAAGCAATGCTGGAAAAACGTGTAAAACGTCAACTGCTtgatg < 1:1189503/46‑1 (MQ=255) cTGGAAGCAATGCTGGAAAAACGTGTAAAACGTCAACTGCTtgatg < 1:159121/46‑1 (MQ=255) cTGGAAGCAATGCTGGAAAAACGTGTAAAACGTCAACTGCTtgatg < 1:4113189/46‑1 (MQ=255) cTGGAAGCAATGCTGGAAAAACGTGTAAAACGTCAACTGCTTGATGAAGTGCAGTCTATTTGTCCACCg < 1:2920010/69‑1 (MQ=255) cTGGAAGCAATGCTGGAAAAACGTGTAAAACGTCAACTGCTTGATGAAGTGCAGTCTATTTGTCCACCGCa < 1:1868228/71‑1 (MQ=255) cTGGAAGCAATGCTGGAAAAACGTGTAAAACGTCAACTGCTTGATGAAGTGCAGTCTATTTGTCCACCGCa < 1:1521556/71‑1 (MQ=255) tGGAAGCAATGCTGGAAAAACGTGTAAAACGTCAACTGCTTGATGAAGTGCAGTCTATTTGTCCACCGCa < 1:3431219/70‑1 (MQ=255) gCAATGCTGGAAAAACGTGTAAAACGTCAACTGCTtgatg < 1:4084853/40‑1 (MQ=255) gCAATGCTGGAAAAACGTGTAAAACGTCAACTGCTtgatg < 1:753995/40‑1 (MQ=255) gCAATGCTGGAAAAACGTGTAAAACGTCAACTGCTtgatg < 1:3759364/40‑1 (MQ=255) cAATGCTGGAAAAACGTGTAAAACGTCAACTGCtt < 1:1932205/35‑1 (MQ=255) cAATGCTGGAAAAACGTGTAAAACGTCAACTGCtt < 1:3815667/35‑1 (MQ=255) cAATGCTGGAAAAACGTGTAAAACGTCAACTGCtt < 1:2378435/35‑1 (MQ=255) aaTGCTGGAAAAACGTGTAAAACGTCAACTGCTTGATGAAg > 1:1871109/1‑41 (MQ=255) | GTGCTGGTTACACACTCATCTAAAAACTCTATTGAAAACCACTTTGATACCAGTTTTGAACTGGAAGCAATGCTGGAAAAACGTGTAAAACGTCAACTGCTTGATGAAGTGCAGTCTATTTGTCCACCGCA > minE/843307‑843437 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |