| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 138,172 | A→G | 24.7% | N665N (AAT→AAC) | htrE ← | predicted outer membrane usher protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 138,172 | 0 | A | G | 24.7% | 130.0 / 48.1 | 73 | N665N (AAT→AAC) | htrE | predicted outer membrane usher protein |
| Reads supporting (aligned to +/- strand): ref base A (26/29); new base G (2/16); total (28/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.99e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.24e-01 | |||||||||||
AGTCCACCGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAGCTGGCATAACCCCCAACA > minE/138107‑138235 | agtcCACCGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTg > 1:1322617/1‑70 (MQ=255)agtcAACCGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTg > 1:1024353/1‑70 (MQ=255) cACCGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCAGaa < 1:360727/71‑1 (MQ=255) cACCGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCAGaa < 1:1568412/71‑1 (MQ=255) cACCGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGa < 1:1004943/70‑1 (MQ=255) cACCGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGa < 1:4011949/70‑1 (MQ=255) cACCGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGa < 1:3447639/70‑1 (MQ=255) cACCGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGa < 1:2252189/70‑1 (MQ=255) cTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTGa < 1:909559/71‑1 (MQ=255) cTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTGa < 1:1949889/71‑1 (MQ=255) cTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTGa < 1:1021381/71‑1 (MQ=255) cTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGa < 1:3988662/71‑1 (MQ=255) cTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGa < 1:1666579/71‑1 (MQ=255) cTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGa < 1:2888979/71‑1 (MQ=255) aTGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGGCGGCTGTTATCGCTGTTTGCAGAAATTg < 1:3359115/68‑1 (MQ=255) aTGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTg < 1:3731516/68‑1 (MQ=255) aTGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTg < 1:2230944/68‑1 (MQ=255) aTGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTg < 1:2512131/68‑1 (MQ=255) tGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGa < 1:1462069/68‑1 (MQ=255) tGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGa < 1:1269570/68‑1 (MQ=255) tGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAAcc < 1:36881/71‑1 (MQ=255) gCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAAc > 1:480915/1‑69 (MQ=255) cAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAAc > 1:2224246/1‑68 (MQ=255) cAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAAc > 1:298784/1‑68 (MQ=255) aTACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTAt > 1:2995693/1‑51 (MQ=255) aTACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTAt > 1:3056014/1‑51 (MQ=255) aTACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTAt > 1:593930/1‑51 (MQ=255) aCAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGaaa > 1:3105584/1‑58 (MQ=255) cAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGc > 1:1383551/1‑71 (MQ=255) aaaCCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGt > 1:2946375/1‑71 (MQ=255) aaaCCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGt > 1:2444408/1‑71 (MQ=255) aaaCCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGt > 1:2481397/1‑71 (MQ=255) aaaCCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGt > 1:1089079/1‑71 (MQ=255) aaaCCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGt > 1:2449067/1‑71 (MQ=255) aaaCCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGt > 1:440664/1‑71 (MQ=255) accaccGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTc < 1:401499/71‑1 (MQ=255) accaccGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTc < 1:4110424/71‑1 (MQ=255) accaccGGCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTc < 1:3221799/71‑1 (MQ=255) caccGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTg < 1:3751406/45‑1 (MQ=255) accGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGa < 1:2422110/54‑1 (MQ=255) ccGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTACCCCa > 1:259752/1‑71 (MQ=255) cGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGCGTTCCCCa < 1:1517451/70‑1 (MQ=255) cGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGCGTTCCCCAt < 1:2984975/71‑1 (MQ=255) ggTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGt < 1:497823/70‑1 (MQ=255) cTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACt < 1:3005864/70‑1 (MQ=255) cTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACt < 1:826059/70‑1 (MQ=255) cTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACt < 1:126325/70‑1 (MQ=255) gagagaAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAAc > 1:1860855/1‑44 (MQ=255) gagagaAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAAc > 1:2024778/1‑44 (MQ=255) gagagaAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAAc > 1:2839821/1‑44 (MQ=255) gagagaAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAAc > 1:3359931/1‑44 (MQ=255) gagagaAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAAc > 1:3568417/1‑44 (MQ=255) agagaAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGa > 1:1424877/1‑65 (MQ=255) gagaAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAg < 1:1471033/71‑1 (MQ=255) gagaAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCCGCGTTCCCCATGGTGACTCAt < 1:1792978/69‑1 (MQ=255) agaAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAGc < 1:3382725/71‑1 (MQ=255) gaAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAGc < 1:3735753/70‑1 (MQ=255) aaaCTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAGCTg > 1:1004109/1‑71 (MQ=255) aaaCTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAGCTg > 1:2390268/1‑71 (MQ=255) aaaCTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAGCTg > 1:1489118/1‑71 (MQ=255) aaCTTGACGGCTGTTATCGCTATTTGCAGAAATTg < 1:1700463/35‑1 (MQ=255) aaCTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAGCTgg < 1:1809256/71‑1 (MQ=255) aaCTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAGCTgg < 1:1461584/71‑1 (MQ=255) gACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAGCTGGCAt < 1:1062092/69‑1 (MQ=255) gACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAGCTGGCAt < 1:3371099/69‑1 (MQ=255) gACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAGCTGGCAt < 1:2517512/69‑1 (MQ=255) aCGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAGCTGGCATaa < 1:3340995/70‑1 (MQ=255) aCGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAGCTGGCATaa < 1:3807190/70‑1 (MQ=255) aCGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAGCTGGCATaa < 1:2105360/70‑1 (MQ=255) cTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAGCTGGCATAAccccc < 1:1461360/71‑1 (MQ=255) tATCGCTGTTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAGCTGGCATAACCCCCAACa > 1:1541597/1‑71 (MQ=255) tATCGCTATTTGCAGAAATTGAACCTTCCAGCGTTCCCCATGGTGACTCATa < 1:2384224/52‑1 (MQ=255) aTCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAGCTGGCATAACCCCCAACa > 1:195522/1‑70 (MQ=255) | AGTCCACCGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAGCTGGCATAACCCCCAACA > minE/138107‑138235 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |