| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,026,348 | T→C | 25.7% | E175G (GAG→GGG) | malI ← | DNA‑binding transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,026,348 | 0 | T | C | 25.7% | 118.5 / 48.0 | 70 | E175G (GAG→GGG) | malI | DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base T (25/27); new base C (9/9); total (34/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01 | |||||||||||
GCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAACT > minE/1026279‑1026412 | gCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTc > 1:2289952/1‑71 (MQ=255) cGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGt > 1:3802429/1‑71 (MQ=255) cGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGt > 1:3809990/1‑71 (MQ=255) cGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGt > 1:1681176/1‑71 (MQ=255) ttAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTcaac < 1:9010/71‑1 (MQ=255) ttAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTcaac < 1:3332150/71‑1 (MQ=255) ttAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTcaac < 1:1193827/71‑1 (MQ=255) aGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACtgt > 1:5905/1‑71 (MQ=255) ggAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACtgtg < 1:1783477/71‑1 (MQ=255) aCTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAg > 1:1585760/1‑71 (MQ=255) aCTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAg > 1:2862742/1‑71 (MQ=255) ttGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCt > 1:2912141/1‑71 (MQ=255) ttGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCt > 1:3073712/1‑71 (MQ=255) ttGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCt > 1:2341898/1‑71 (MQ=255) ttGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCt > 1:3199929/1‑71 (MQ=255) tGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTcaacaa > 1:3973546/1‑59 (MQ=255) tGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTcaacaa > 1:4021642/1‑59 (MQ=255) cTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCCCCGTCAACAACTGTGCAg < 1:2792432/63‑1 (MQ=255) ccagccagGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCAtgttg < 1:1249739/71‑1 (MQ=255) agccagGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTcaaca > 1:1860546/1‑49 (MQ=255) agccagGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTcaaca > 1:2671044/1‑49 (MQ=255) agccagGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTcaaca > 1:3566905/1‑49 (MQ=255) agccagGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTcaaca > 1:2215361/1‑49 (MQ=255) agccagGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTcaaca > 1:810807/1‑49 (MQ=255) agccagGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTcaaca > 1:2639166/1‑49 (MQ=255) agGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTcaaca < 1:570329/45‑1 (MQ=255) agGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTcaaca < 1:3685970/45‑1 (MQ=255) ggCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTcaac < 1:1117262/43‑1 (MQ=255) ggCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTcaac < 1:3529230/43‑1 (MQ=255) ggCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTcaac < 1:738691/43‑1 (MQ=255) ggCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTcaac < 1:2669062/43‑1 (MQ=255) ggCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTcaac < 1:1158154/43‑1 (MQ=255) ggCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTcaac < 1:1920745/43‑1 (MQ=255) ggCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTcaac < 1:1994345/43‑1 (MQ=255) gCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTcaac < 1:2036313/42‑1 (MQ=255) gCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTcaac < 1:3281870/42‑1 (MQ=255) ccGCTGATGCCCATTGCGAATGAGATGCCCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCg > 1:173059/1‑71 (MQ=255) cGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCAGCATGTTGTCCGGGCGAACCg > 1:3718180/1‑70 (MQ=255) cGCTGATGCCCATTGCGAATGAGATGCCCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCg > 1:1018610/1‑70 (MQ=255) aTGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATc < 1:273679/69‑1 (MQ=255) aTTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAAcatcat > 1:3878281/1‑71 (MQ=255) ttGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGa < 1:4087396/54‑1 (MQ=255) ttGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGa < 1:2835834/54‑1 (MQ=255) ttGCGAATGAGATGCTCCGTCAACAACTGTGCAGACTGCATGTTGTCCGGGCGa < 1:526350/54‑1 (MQ=255) tGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCaa > 1:26263/1‑63 (MQ=255) gCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACatcatc > 1:1219760/1‑69 (MQ=255) gCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACatcatc > 1:156952/1‑69 (MQ=255) gCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGG‑‑GAACCGTATCAACATCATCAAg < 1:3796741/70‑1 (MQ=255) gCGAATGAGATGCCCCGTCAACAACTGTGCAGCCTGCAtgttgt > 1:1268166/1‑44 (MQ=255) gCGAATGAGATGCCCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACatcatc > 1:2766519/1‑69 (MQ=255) gCGAATGAGATGCCCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACatcatc > 1:1524614/1‑69 (MQ=255) gCGAATGAGATGCCCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACatcatc > 1:3111235/1‑69 (MQ=255) gCGAATGAGATGCCCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACatcatc > 1:2329281/1‑69 (MQ=255) gCGAATGAGATGCCCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACatcatc > 1:3263402/1‑69 (MQ=255) cGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAg < 1:2520512/71‑1 (MQ=255) cGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAg < 1:2455631/71‑1 (MQ=255) cGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAg < 1:2136558/71‑1 (MQ=255) cGAATGAGATGCCCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAg < 1:3597414/71‑1 (MQ=255) cGAATGAGATGCCCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAg < 1:2328823/71‑1 (MQ=255) cGAATGAGATGCCCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAg < 1:3515791/71‑1 (MQ=255) gAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCAtgttg < 1:1271128/41‑1 (MQ=255) aaTGAGATGCCCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGAt < 1:491190/71‑1 (MQ=255) aaTGAGATGCCCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGAt < 1:3088400/71‑1 (MQ=255) aaTGAGATGCCCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGAt < 1:884375/71‑1 (MQ=255) aaTGAGATGCCCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGAt < 1:260589/71‑1 (MQ=255) aTGAGATGCCCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGAt > 1:2467470/1‑70 (MQ=255) tGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATaa > 1:4037015/1‑71 (MQ=255) agaTGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAAc < 1:590456/70‑1 (MQ=255) agaTGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAAc < 1:3582209/70‑1 (MQ=255) agaTGCCCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAACt < 1:552990/71‑1 (MQ=255) | GCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTGCATGTTGTCCGGGCGAACCGTATCAACATCATCAAGATAACT > minE/1026279‑1026412 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |