| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,027,446 | A→G | 20.8% | N134S (AAC→AGC) | malX → | fused maltose and glucose‑specific PTS enzyme IIBC components |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,027,446 | 0 | A | G | 20.8% | 212.7 / 53.1 | 101 | N134S (AAC→AGC) | malX | fused maltose and glucose‑specific PTS enzyme IIBC components |
| Reads supporting (aligned to +/- strand): ref base A (46/34); new base G (14/7); total (60/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.18e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
Only 100 of 102 total aligned reads displayed.
GTAAACTTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCAGTCGATCGATACCGGGATCCTCGGTGCGGTGATCGCCGGTATTATC > minE/1027379‑1027516 | gTAAACTTCTTGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACAt < 1:2146784/71‑1 (MQ=255)gTAAACTTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACAt < 1:3500241/71‑1 (MQ=255)gTAAACTTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACAt < 1:2151589/71‑1 (MQ=255)gTAAACTTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACAt < 1:1768887/71‑1 (MQ=255) tAAGCTTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATc > 1:1076485/1‑71 (MQ=255) tAAACTTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACAt < 1:3734330/70‑1 (MQ=255) cTTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATc > 1:2074653/1‑67 (MQ=255) cTTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCa > 1:220155/1‑69 (MQ=255) cTTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCa > 1:2863630/1‑69 (MQ=255) cTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAGCATCCagag < 1:375168/69‑1 (MQ=255) cTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAGCATCCAGAGCa > 1:3318627/1‑71 (MQ=255) cTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAGCATCCAGAGCa > 1:1893643/1‑71 (MQ=255) cTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCa > 1:3641825/1‑71 (MQ=255) gTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAGCATCCAGAGCa < 1:123633/68‑1 (MQ=255) gTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCAtt < 1:2080760/70‑1 (MQ=255) ttGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCt > 1:1066950/1‑71 (MQ=255) ttGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCt > 1:1821297/1‑71 (MQ=255) gACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCAtt > 1:1843799/1‑67 (MQ=255) gACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCAtt > 1:2119556/1‑67 (MQ=255) gACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCAtt > 1:1373222/1‑67 (MQ=255) gACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCAtt > 1:3590419/1‑67 (MQ=255) gACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAAAATCCAGAGCAtt > 1:2682606/1‑67 (MQ=255) aTAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATc > 1:752795/1‑71 (MQ=255) tAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTggg < 1:489758/67‑1 (MQ=255) tAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTggg < 1:3466470/67‑1 (MQ=255) tAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTggg < 1:3549622/67‑1 (MQ=255) aaaGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATc > 1:1933741/1‑69 (MQ=255) aaGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAGCATCCAGAGCATTCTTGGGATCCa > 1:3286947/1‑70 (MQ=255) aaGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCAg > 1:1994273/1‑71 (MQ=255) aaGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCAg > 1:2605766/1‑71 (MQ=255) aaGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCAg > 1:1065972/1‑71 (MQ=255) aaGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCAg > 1:3830685/1‑71 (MQ=255) aTTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCAtt < 1:327981/54‑1 (MQ=255) ttCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAGCATCCAGAGCATTc < 1:2424142/54‑1 (MQ=255) ttCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAGCATCCAGAGCATTc < 1:1300849/54‑1 (MQ=255) ttCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCagag < 1:2677142/49‑1 (MQ=255) ttCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCagag < 1:1726121/49‑1 (MQ=255) ttCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCAGtcgatc < 1:3975504/71‑1 (MQ=255) cTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAAc > 1:1965050/1‑39 (MQ=255) cTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAAc > 1:1431968/1‑39 (MQ=255) cTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAAc > 1:2517240/1‑39 (MQ=255) cTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAAc > 1:3913346/1‑39 (MQ=255) cTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCaga > 1:308277/1‑46 (MQ=255) cTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCaga > 1:2426152/1‑46 (MQ=255) cTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCaga > 1:1770142/1‑46 (MQ=255) cTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCaga > 1:4082340/1‑46 (MQ=255) cTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCAtt > 1:1296096/1‑51 (MQ=255) cTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCAGtcgatcga > 1:1671289/1‑71 (MQ=255) cTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCAGtcgatcg > 1:381892/1‑70 (MQ=255) cTGCAAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCAtt > 1:3093014/1‑51 (MQ=255) aaCCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCAGTCGATCGATACCg > 1:3848404/1‑71 (MQ=255) aaCCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCAGTCGATCGATACCg > 1:2701454/1‑71 (MQ=255) aaCCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCAGTCGATCGATACCg > 1:731095/1‑71 (MQ=255) aCCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTg < 1:2529009/49‑1 (MQ=255) aCCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTg < 1:2573156/49‑1 (MQ=255) aCCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTg < 1:1552304/49‑1 (MQ=255) aCCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTg < 1:2765221/49‑1 (MQ=255) aCCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCAGtcgatc < 1:1520344/63‑1 (MQ=255) aCCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCAGtcgatc < 1:2824325/63‑1 (MQ=255) aCCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCAGtcgatc < 1:1697674/63‑1 (MQ=255) aCCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCAGTCGATCGATAc < 1:1939034/68‑1 (MQ=255) aCCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCAGTCGATCGATAc < 1:2769180/68‑1 (MQ=255) cACGGATTCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTgcg > 1:3486517/1‑47 (MQ=39) cACGGATGCCGCGGTTCTGAAAGCCAATAGCATCCAGAGCATTCTTGTGATCCAGTCGATCGATa > 1:2998438/1‑65 (MQ=255) cACGGATGCCGCGGTTCTGAAAGCCAATAGCATCCAGAGCATTCTTGGGATCCAGTCGATCGATa > 1:751445/1‑65 (MQ=255) cACGGATGCCGCGGTTCTGAAAGCCAATAGCATCCAGAGCATTCTTGGGATCCAGTCGATCGATa > 1:3673246/1‑65 (MQ=255) cACGGATGCCGCGGTTCTGAAAGCCAATAGCATCCAGAGCATTCTTGGGATACAGTCGATCGATa > 1:3200216/1‑65 (MQ=255) cACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTggg > 1:2950142/1‑49 (MQ=255) cACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTggg > 1:680156/1‑49 (MQ=255) cACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTggg > 1:1125007/1‑49 (MQ=255) cACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTggg > 1:1260421/1‑49 (MQ=255) cACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTggg > 1:1439863/1‑49 (MQ=255) cACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCAGtcgatcga > 1:2709746/1‑63 (MQ=255) cACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCAGTCGATCGATACCGGGa < 1:2869304/71‑1 (MQ=255) ccGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCAGTCGATCGATACCGGGATCCTcggt > 1:771534/1‑71 (MQ=255) ccGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCAGTCGATCGATACCGGGATCCTcggt > 1:3354006/1‑71 (MQ=255) ccGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCAGTCGATCGATACCGGGATCCTcggt > 1:272333/1‑71 (MQ=255) cgGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCAGTCGATCGATACCGGGATCCTcggtgcg < 1:1667436/71‑1 (MQ=255) ttCTGAAAGCCAATAGCATCCAGAGCATTCTTGGGATCCAGTCGATCGATACCGGGATCCTcggtgcggtg < 1:3438840/71‑1 (MQ=255) cTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCAGTCGATCGATACCGGGATCCTCGGTGCGGTGAt > 1:3583548/1‑71 (MQ=255) cTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCAGTCGATCGATACCGGGATCCTCGGTGCGGTGAt > 1:2964947/1‑71 (MQ=255) cTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCAGTCGATCGATACCGGGATCCTCGGTGCGGTGAt > 1:1219479/1‑71 (MQ=255) gCCAATAACATCCAGAGCATTCTTGGGATCCAGtcgatc < 1:1843949/39‑1 (MQ=255) gCCAATAACATCCAGAGCATTCTTGGGATCCAGtcgatc < 1:1137630/39‑1 (MQ=255) gCCAATAACATCCAGAGCATTCTTGGGATCCAGTCGATCGATACCGGGATCCTCGGTGCGGTGATCTCCgg < 1:407818/71‑1 (MQ=255) gCCAATAACATCCAGAGCATTCTTGGGATCCAGTCGATCGATACCGGGATCCTCGGTGCGGTGATCGCCgg < 1:1978624/71‑1 (MQ=255) ccAATAACATCCAGAGCATTCTTGGGATCCAGTCGATCGATACCGGGATCCTCGGTGCGGTGATCGCCgg < 1:2644478/70‑1 (MQ=255) cAATAGCATCCAGAGCATTCTTGGGATCCAGTCGATCGATa > 1:124930/1‑41 (MQ=255) cAATAGCATCCAGAGCATTCTTGGGATCCAGTCGATCGATa > 1:1117235/1‑41 (MQ=255) cAATAGCATCCAGAGCATTCTTGGGATCCAGTCGATCGATa > 1:3398201/1‑41 (MQ=255) aaTAGCATCCAGAGCATTCTTGGGATCCAGTCGATCGATACCGGGATCCTCGGTGCGGTGATCGCCGGtat > 1:1459015/1‑71 (MQ=255) aaTAGCATCCAGAGCATTCTTGGGATCCAGTCGATCGATACCGGGATCCTCGGTGCGGTGATCGCCGGtat > 1:4073052/1‑71 (MQ=255) aaTAGCATCCAGAGCATTCTTGGGATCCAGTCGATCGATACCGGGATCCTCGGTGCGGTGATCGCCGGtat > 1:621791/1‑71 (MQ=255) aaTAGCATCCAGAGCATTCTTGGGATCCAGTCGATCGATACCGGGATCCTCGGTGCGGTGATCGCCGGtat > 1:905992/1‑71 (MQ=255) aTAGCATCCAGAGCATTCTTGGGATCCAGTCGATCGATACCGGGATCCTCGGTGCGGTGATCGCCGGtatt < 1:1516185/71‑1 (MQ=255) tAACATCCAGAGCATTCTTGGGATCCAGTcgatcgat < 1:242257/37‑1 (MQ=255) tAACATCCAGAGCATTCTTGGGATCCAGTcgatcgat < 1:613755/37‑1 (MQ=255) tAACATCCAGAGCATTCTTGGGATCCAGTcgatcgat < 1:2989040/37‑1 (MQ=255) tAACATCCAGAGCATTCTTGGGATCCAGTcgatcgat < 1:1575193/37‑1 (MQ=255) aCATCCAGAGCATTCTTGGGATCCAGTCGATCGATACCGGGATCCTCGGTGCGGTGATCGCCGGTATTATc < 1:188925/71‑1 (MQ=255) | GTAAACTTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCAGTCGATCGATACCGGGATCCTCGGTGCGGTGATCGCCGGTATTATC > minE/1027379‑1027516 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |