| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,069,991 | G→A | 10.2% | Q296Q (CAG→CAA) | cfa → | cyclopropane fatty acyl phospholipid synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,069,991 | 0 | G | A | 10.2% | 115.5 / 4.3 | 39 | Q296Q (CAG→CAA) | cfa | cyclopropane fatty acyl phospholipid synthase |
| Reads supporting (aligned to +/- strand): ref base G (13/22); new base A (2/2); total (15/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.31e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01 | |||||||||||
AATGTTGATCCCTGGATTAATAAATATATTTTTCCGAACGGTTGCCTGCCCTCTGTACGCCAGATTGCTCAGTCCAGCGAACCCCACTTTGTGATGGAAGACTGGCATAACTTCGGTGCTGATTACGATAC > minE/1069929‑1070059 | aaTGTTGATCCCTGGATTAATAAATATATTTTTCCGAACGGTTGCCTGCCCTCTGTACGCCAGATTg < 1:524713/67‑1 (MQ=255)aaTGTTGATCCCTGGATTAATAAATATATTTTTCCGAACGGTTGCCTGCCCTCTGTACGCCAGATTg < 1:1406463/67‑1 (MQ=255)aaTGTTGATCCCTGGATTAATAAATATATTTTTCCGAACGGTTGCCTGCCCTCTGTACGCCAGATTg < 1:2112223/67‑1 (MQ=255)aaTGTTGATCCCTGGATTAATAAATATATTTTTCCGAACGGTTGCCTGCCCTCTGTACGCCAGATTGCt < 1:3137192/69‑1 (MQ=255)aaTGTTGATCCCTGGATTAATAAATATATTTTTCCGAACGGTTGCCTGCCCTCTGTACGCCAGATTGCt < 1:2382301/69‑1 (MQ=255)aaTGTTGATCCCTGGATTAATAAATATATTTTTCCGAACGGTTGCCTGCCCTCTGTACGCCAGATTGCt > 1:3598672/1‑69 (MQ=255) tGTTGATCCCTGGATTAATAAATATATTTTTCCGAACGGTTGCCTGCCCTCTGTACGCCAGATTGCTCAGt > 1:3589274/1‑71 (MQ=255) ttGATCCCTGGATTAATAAATATATTTTTCCGAACGGTTGCCTGCCCTCTGTACTCCAGATTGCTCAGTc > 1:1144035/1‑70 (MQ=255) ttGATCCCTGGATTAATAAATATATTTTTCCGAACGGTTGCCTGCCCTCTGTACGCCAGATTGCTCAGTcc < 1:2674974/71‑1 (MQ=255) ttGATCCCTGGATTAATAAATATATTTTTCCGAACGGTTGCCTGCCCTCTGTACGCCAGATTGCTCAGTcc < 1:3478050/71‑1 (MQ=255) ttGATCCCTGGATTAATAAATATATTTTTCCGAACGGTTGCCTGCCCTCTGTACGCCAGATTGCTCAGTcc < 1:3689329/71‑1 (MQ=255) ttGATCCCTGGATTAATAAATATATTTTTCCGAACGGTTGCCTGCCCTCTGTACGCCAGATTGCTCAGTcc < 1:1200514/71‑1 (MQ=255) ttGATCCCTGGATTAATAAATATATTTTTCCGAACGGTTGCCTGCCCTCTGTACGCCAGATTGCTCAGTc > 1:140588/1‑70 (MQ=255) tGATCCCTGGATTAATAAATATATTTTTCCGAACGGTTGCCTGCCCTCTGTACGCCAGATTGCTCAGTcc < 1:1472395/70‑1 (MQ=255) tGATCCCTGGATTAATAAATATATTTTTCCGAACGGTTGCCTGCCCTCTGTACGCCAAATTGCTCAGTc > 1:3501178/1‑69 (MQ=255) gATCCCTGGATTAATAAATATATTTTTCCGAACGGTTGCCTGCCCTCTGTACGCCAGATTGCTCAGTCCAg > 1:417075/1‑71 (MQ=255) gATCCCTGGATTAATAAATATATTTTTCCGAACGGTTGCCTGCCCTCTGTACGCCAAATTGCTCAGTCCa < 1:2691102/70‑1 (MQ=255) tGGATTAATAAATATATTTTTCCGAACGGTTGCCTGCCCTCTGTACGCCAGATTGCTCAGTCCa > 1:3625852/1‑64 (MQ=255) tGGATTAATAAATATATTTTTCCGAACGGTTGCCTGCCCTCTGTACGCCAGATTGCTCAGTCCAGCGAAcc > 1:2888989/1‑71 (MQ=255) gATTAATAAATATATTTTTCCGAACGGTTGCCTGCCCTCTGTACGCCAGATTGCTCAGTCCAGCGAAccc > 1:256486/1‑70 (MQ=255) gATTAATAAATATATTTTTCCGAACGGTTGCCTGCCCTCTGTACGCCAAATTGCTCAGTCCAGCGAAccc > 1:2618871/1‑70 (MQ=255) aataTATTTTTCCGAACGGTTGCCTGCCCTCTGTACGCCAGATTGCTCAGTCCAGCGAAGCCCACTTtgt < 1:857549/70‑1 (MQ=255) aataTATTTTTCCGAACGGTTGCCTGCCCTCTGTACGCCAGATTGCTCAGTCCAGCGAACCCCACTTtgt < 1:1618320/70‑1 (MQ=255) aataTATTTTTCCGAACGGTTGCCTGCCCTCTGTACGCCAGATTGCTCAGTCCAGCGAACCCCACTTtgt < 1:638818/70‑1 (MQ=255) aataTATTTTTCCGAACGGTTGCCTGCCCTCTGTACGCCAGATTGCTCAGTCCAGCGAACCCCACTTtgt < 1:3299365/70‑1 (MQ=255) tatTTTTCCGAACGGTTGCCTGCCCTCTGTACGCCAGATTGc < 1:523152/42‑1 (MQ=255) tttttCCGAACGGTTGCCTGCCCTCTGTACGCCAGATTGCTCAGTCCAGCGaa < 1:3198452/53‑1 (MQ=255) tttttCCGAACGGTTGCCTGCCCTCTGTACGCCAGATTGCTCAGTCCAGCGaa > 1:157595/1‑53 (MQ=255) tttttCCGAACGGTTGCCTGCCCTCTGTACGCCAGATTGCTCAGTCCAGCGAACCCCACTTTGTGATGGaa < 1:2241332/71‑1 (MQ=255) tttttCCGAACGGTTGCCTGCCCTCTGTACGCCAGATTGCTCAGTCCAGCGAACCCCACTTTGTGATGGaa < 1:2203055/71‑1 (MQ=255) tttttCCGAACGGTTGCCTGCCCTCTGTACGCCAGATTGCTCAGTCCAGCGAACCCCACTTTGTGATGGaa < 1:765835/71‑1 (MQ=255) tttttCCGAACGGTTGCCTGCCCTCTGTACGCCAAATTGCTCAGTCCAGCGAACCCCACTTTGTGATGGaa < 1:1432966/71‑1 (MQ=255) ccTCTGTACGCCAGATTGCTCAGTCCAGCGAACCCCACTTTGTGATGGAAGACTGGCATAACTTCGGTGCt < 1:2632528/71‑1 (MQ=255) tACGCCAGATTGCTCAGTCCAGCGAACCCCACTTTGTGATGGAAGACTGGCa > 1:3115183/1‑52 (MQ=255) tACGCCAGATTGCTCAGTCCAGCGAACCCCACTTTGTGATGGAAGACTGGCa > 1:509470/1‑52 (MQ=255) tACGCCAGATTGCTCAGTCCAGCGAACCCCACTTTGTGATGGAAGACTGGCATAACTTCGGTGCTGATTAc < 1:3191098/71‑1 (MQ=255) tACGCCAGATTGCTCAGTCCAGCGAACCCCACTTTGTGATGGAAGACTGGCATAACTTCGGTGCTGATTAc > 1:3714958/1‑71 (MQ=255) tACGCCAGATAGCTCAGTCCAGCGAACCCCACTTTGTGATGGAAGACTGGCa > 1:2505174/1‑52 (MQ=255) cAGATTGCTCAGTCCAGCGAACCCCACTTTGTGATGGAAGACTGGCATAACTTCGGTGCTGATTACGAtac < 1:518711/71‑1 (MQ=255) gATTTCTCAGTCCAGCGAACCCCACTTTGTGATgg < 1:2266942/35‑1 (MQ=255) gATTGCTCAGTCCAGCGAACCCCACTTTGTGATgg < 1:1227568/35‑1 (MQ=255) gATTGCTCAGTCCAGCGAACCCCACTTTGTGATgg < 1:1852031/35‑1 (MQ=255) gATTGCTCAGTCCAGCGAACCCCACTTTGTGATgg < 1:974162/35‑1 (MQ=255) | AATGTTGATCCCTGGATTAATAAATATATTTTTCCGAACGGTTGCCTGCCCTCTGTACGCCAGATTGCTCAGTCCAGCGAACCCCACTTTGTGATGGAAGACTGGCATAACTTCGGTGCTGATTACGATAC > minE/1069929‑1070059 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |