| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,073,671 | C→T | 24.4% | G50S (GGC→AGC) | ydhQ ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,073,671 | 0 | C | T | 24.4% | 83.3 / 25.8 | 45 | G50S (GGC→AGC) | ydhQ | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (14/20); new base T (6/5); total (20/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
AGCGTGCCATTGTTACCGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACTTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCTTCAGTA > minE/1073607‑1073735 | aGCGTGCCATTGTTACCGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACTTTCAACCAGCTAGccc > 1:2625274/1‑70 (MQ=255) gCGTGCCATTGTTACCGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACTTTCAACCAGCTAGcccc < 1:3333390/70‑1 (MQ=255) gCGTGCCATTGTTACCGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACTTTCAACCAGCTAGcccc < 1:1677669/70‑1 (MQ=255) gCGTGCCATTGTTACCGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACTTTCAACCAGCTAGcccc < 1:670067/70‑1 (MQ=255) tGTTACCGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACTTTCAACCAGCCAGCCCCCGTGACCACg > 1:3595290/1‑71 (MQ=255) gTTACCGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACTTTCAACCAGCCAGCCCCCGTGACCACgg > 1:3331846/1‑71 (MQ=255) gTTACCGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACTTTCAACCAGCCAGCCCCCGTGACCACgg > 1:2833418/1‑71 (MQ=255) ccGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACTTTCAACCAGCCAGCCCCCGTGACCATGGTAtt < 1:2573750/71‑1 (MQ=255) tgtgCATTTGGCGATCCCACCGGTTGCCACTTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGctc > 1:2271957/1‑71 (MQ=255) tgCATTTGGCGATCCCACCGGTTGCCACTTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGctct < 1:2173261/70‑1 (MQ=255) tttGGCGATCCCACCGGTTGCCACTTTCAACCAGCCAGCCCCCGTGAc < 1:2576868/48‑1 (MQ=255) tttGGCGATCCCACCGGTTGCCACTTTCAACCAGCCAGCCCCCGTGACCACGGTAtt < 1:1337583/57‑1 (MQ=255) tttGGCGATCCCACCGGTTGCCACTTTCAACCAGCCAGCCCCCGTGACCACGGTAtt < 1:3829716/57‑1 (MQ=255) tttGGCGATCCCACCGGTTGCCACTTTCAACCAGCCAGCCCCCGTGACCACGGTAtt < 1:3257521/57‑1 (MQ=255) tttGGCGATCCCACCGGTTGCCACTTTCAACCAGCCAGCCCCCGTGACCACGGTAtt < 1:2906338/57‑1 (MQ=255) tttGGCGATCCCACCGGTTGCCACTTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGc < 1:2782318/71‑1 (MQ=255) tGGCGATCCCACCGGTTGCCACTTTCAACCAGCCAGCCCCCGTGa > 1:2351355/1‑45 (MQ=255) tGGCGATCCCACCGGTTGCCACTTTCAACCAGCCAGCCCCCGTGa > 1:1126862/1‑45 (MQ=255) tGGCGATCCCACCGGTTGCCACTTTCAACCAGCCAGCCCCCGTGa > 1:2608161/1‑45 (MQ=255) tGGCGATCCCACCGGTTGCCACTTTCAACCAGCCAGCCCCCGTGa > 1:659245/1‑45 (MQ=255) tGGCGATCCCACCGGTTGCCACTTTCAACCAGCCAGCCCCCGTGACCACGGTAtttt > 1:2501768/1‑57 (MQ=255) tGGCGATCCCACCGGTTGCCACTTTCAACCAGCCAGCCCCCGTGACCACGGTAtttt > 1:1432946/1‑57 (MQ=255) gATCCCACCGGTTGCCACTTTCAACCAGCCAGCCCCCGTGACCACGGTAtt < 1:2750098/51‑1 (MQ=255) gATCCCACCGGTTGCCACTTTCAACCAGCCAGCCCCCGTGACCACGGTAtt < 1:342550/51‑1 (MQ=255) gATCCCACCGGTTGCCACTTTCAACCAGCCAGCCCCCGTGACCACGGTAtt < 1:2378052/51‑1 (MQ=255) gATCCCACCGGTTGCCACTTTCAACCAGCCAGCCCCCGTGACCACGGTAtt < 1:2289192/51‑1 (MQ=255) tCCCACCGGTTGCCACTTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTc > 1:1832922/1‑70 (MQ=255) cGGTTGCCACTTTCAACCAGCCAGCCCCCGTGACCACGGTAtt < 1:2284530/43‑1 (MQ=255) cGGTTGCCACTTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATc < 1:3514359/71‑1 (MQ=255) cTTTCAACCAGCTAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCt > 1:909830/1‑71 (MQ=255) cTTTCAACCAGCTAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCt > 1:1363319/1‑71 (MQ=255) cTTTCAACCAGCTAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCt > 1:565291/1‑71 (MQ=255) cTTTCAACCAGCTAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCt > 1:3641074/1‑71 (MQ=255) cTTTCAACCAGCTAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCt > 1:2257858/1‑71 (MQ=255) cTTTCAACCAGCCAGCCCCCGTGAGCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCt > 1:3440647/1‑71 (MQ=255) cTTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCt > 1:4102344/1‑71 (MQ=255) cTTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCt > 1:4085024/1‑71 (MQ=255) tCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGctct < 1:712814/40‑1 (MQ=255) tCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGctct < 1:2413712/40‑1 (MQ=255) tCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGctct < 1:2052061/40‑1 (MQ=255) tCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCtt < 1:3332576/69‑1 (MQ=255) cAACCAGCTAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCtt < 1:1033640/68‑1 (MQ=255) cAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCtt < 1:3949419/68‑1 (MQ=255) cAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCtt < 1:3776220/68‑1 (MQ=255) aCCAGCTAGCCCCCGTGACCACGCTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCTTCAGTa < 1:2557705/71‑1 (MQ=255) cagctagcCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATc < 1:2493597/54‑1 (MQ=255) cagctagcCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATc < 1:2799182/54‑1 (MQ=255) cagctagcCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATc < 1:634307/54‑1 (MQ=255) cagctagcCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATc < 1:2455946/54‑1 (MQ=255) cagctagcCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATc < 1:2705264/54‑1 (MQ=255) cagctagcCCCCGTGACCACGGTATTTACGGCTCTTCCGCCAGCTTCAACAATc < 1:3452078/54‑1 (MQ=255) | AGCGTGCCATTGTTACCGTACTGTGTGCATTTGGCGATCCCACCGGTTGCCACTTTCAACCAGCCAGCCCCCGTGACCACGGTATTTTCGGCTCTTCCGCCAGCTTCAACAATCAACTCGCCTTCAGTA > minE/1073607‑1073735 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |