| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,082,141 | C→T | 14.1% | D10N (GAT→AAT) | ydhY ← | predicted 4Fe‑4S ferridoxin‑type protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,082,141 | 0 | C | T | 14.1% | 233.5 / 25.7 | 85 | D10N (GAT→AAT) | ydhY | predicted 4Fe‑4S ferridoxin‑type protein |
| Reads supporting (aligned to +/- strand): ref base C (41/32); new base T (5/7); total (46/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.70e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.35e-01 | |||||||||||
GGGCAATTGTTAGACCAGCAAGGCCTTTTCCTGATATACGCAAGAATTCTAATCGCGTTAAGCCAATATCTAATAGTGGACGATCAACCGGGTTCATTTATTTCGCATCCTCTTCGCAAACACAGTGCTGACAATTCAG > minE/1082072‑1082210 | gggCAATTGTTAGACCAGCAAGGCCTTTTCCTGATATACGCAAGAATTCTAATCGCGTTAAGCCAATATCt < 1:3804766/71‑1 (MQ=255)gggCAATTGTTAGACCAGCAAGGCCTTTTCCTGATATACGCAAGAATTCTAATCGCGTTAAGCCAATATCt < 1:2851478/71‑1 (MQ=255) gCAATTGTTAGACCAGCAAGGCCTTTTCCTGATATACGCAAGAATTCTAATCGCGTTAAGCCAATATCTaa < 1:1994131/71‑1 (MQ=255) gCAATTGTTAGACCAGCAAGGCCTTTTCCTGATATACGCAAGAATTCTAATCGCGTTAAGCCAATATCTaa < 1:3820272/71‑1 (MQ=255) tGTTAGACCAGCAAGGCCTTTTCCTGATATACGCAAGAATTCTAATCGCGTTAAGCCAATATCTAAt > 1:2804225/1‑67 (MQ=255) gTTAGACCAGCAAGGCCTTTTCCTGATATACGCAAGAATTCTAATCGCGTTAAGCCAATATCTAATAGTg > 1:349029/1‑70 (MQ=255) gTTAGACCAGCAAGGCCTTTTCCTGATATACGCAAGAATTCTAATCGCGTTAAGCCAATATCTAATAGTg > 1:3599477/1‑70 (MQ=255) ttAGACCAGCAAGGCCTTTTCCTGATATACGCAAGAATTCTAATCGCGTTAAGCCAATATCTAATAGTgg < 1:3353392/70‑1 (MQ=255) tAGACCAGCAAGGCCTTTTCCTGATATACGCAAGAATTCTAATCGCGTTAAGCCAATATCTAATAGTGGAc > 1:1861018/1‑71 (MQ=255) gACCAGCAAGGCCTTTTCCTGATATACTCAAGAATTCTAATCTCGTTAAGCCAATATCTAATAGTGGACg < 1:3146443/70‑1 (MQ=255) gACCAGCAAGGCCTTTTCCTGATATACGCAAGAATTCTAATCGCGTTAAGCCAATATCTAATAGTGGACg < 1:4018236/70‑1 (MQ=255) gACCAGCAAGGCCTTTTCCTGATATACGCAAGAATTCTAATCGCGTTAAGCCAATATCTAATAGTGGACg < 1:355057/70‑1 (MQ=255) gACCAGCAAGGCCTTTTCCTGATATACGCAAGAATTCTAATCGCGTTAAGCCAATATCTAATAGTGGACg < 1:2544462/70‑1 (MQ=255) gACCAGCAAGGCCTTTTCCTGATATACGCAAGAATTCTAATCGCGTTAAGCCAATATCTAATAGTGGACg < 1:1881879/70‑1 (MQ=255) ccAGCATGGCCTTTTCCTGATATACGCAAGAATTCTAATCGCGTTAAGCCAATATCTAATAGTGGACGATc < 1:3352619/71‑1 (MQ=255) cAGCAAGGCCTTTTCCTGATATACGCAAGAATTCTAATCGCGTTAAGCCAATATCTAATAGTGGACGATc > 1:607753/1‑70 (MQ=255) cAGCAAGGCCTTTTCCTGATATACGCAAGAATTCTAATCGCGTTAAGCCAATATCTAATAGTGGACGATCa < 1:1311815/71‑1 (MQ=255) cAGCAAGGCCTTTTCCTGATATACGCAAGAATTCTAATCGCGTTAAGCCAATATCTAATAGTGGACGATCa < 1:3845706/71‑1 (MQ=255) aGCAAGGCCTTTTCCTGATATACGCAAGAATTCTAATCGCGTTAAGCCAATATCTAAt < 1:4007652/58‑1 (MQ=255) aGCAAGGCCTTTTCCTGATATACGCAAGAATTCTAATCGCGTTAAGCCAATATCTAAt < 1:3997754/58‑1 (MQ=255) gCAAGGCCTTTTCCTGATATACGCAAGAATTCTAATCGCGTTAAGCCAATATCTAATAGTGGACGATCAAc > 1:3246963/1‑71 (MQ=255) ccTTTTCCTGATATACGCAAGAATTCTAATCGCGTTAAGCCAATATTTAATa < 1:3217444/52‑1 (MQ=255) ccTTTTCCTGATATACGCAAGAATTCTAATCGCGTTAAGCCAATATTTAATa < 1:1580808/52‑1 (MQ=255) ccTTTTCCTGATATACGCAAGAATTCTAATCGCGTTAAGCCAATATTTAATa < 1:3860631/52‑1 (MQ=255) ccTTTTCCTGATATACGCAAGAATTCTAATCGCGTTAAGCCAATATTTAATa < 1:2901919/52‑1 (MQ=255) ccTTTTCCTGATATACGCAAGAATTCTAATCGCGTTAAGCCAATATTTAATa < 1:696025/52‑1 (MQ=255) ccTTTTCCTGATATACGCAAGAATTCTAATCGCGTTAAGCCAATATTTAATa < 1:814016/52‑1 (MQ=255) ccTTTTCCTGATATACGCAAGAATTCTAATCGCGTTAAGCCAATATTTAATa < 1:1381397/52‑1 (MQ=255) ttttCCTGATATACGCAAGAATTCTAATCGCGTTAAGCCAATATCt < 1:3365756/46‑1 (MQ=255) ttttCCTGATATACGCAAGAATTCTAATCGCGTTAAGCCAATATCt < 1:3576402/46‑1 (MQ=255) ttttCCTGATATACGCAAGAATTCTAATCGCGTTAAGCCAATATCt < 1:3632499/46‑1 (MQ=255) ttttCCTGATATACGCAAGAATTCTAATCGCGTTAAGCCAATATCt < 1:2496270/46‑1 (MQ=255) ttttCCTGATATACGCAAGAATTCTAATCGCGTTAAGCCAATATCt < 1:1640796/46‑1 (MQ=255) ttttCCTGATATACGCAAGAATTCTAATCGCGTTAAGCCAATATCt < 1:4108497/46‑1 (MQ=255) ttttCCTGATATACGCAAGAATTCTAATCGCGTTAAGCCAATATCTAATAGTGGACGATCAACCGGGTTCa > 1:4079654/1‑71 (MQ=255) ttttCCTGATATACGCAAGAATTCTAATCGCGTTAAGCCAATATCTAATAGTGGACGATCAACCGGGTTCa > 1:1626547/1‑71 (MQ=255) ttttCCTGATATACGCAAGAATTCTAATCGCGTTAAGCCAATATCTAATAGTGGACGATCAACCGGGTTCa > 1:2580571/1‑71 (MQ=255) ttCCTGATATACGCAAGAATTCTAATCGCGTTAAGCCAATATCTAATAGTGGACGATCAACCGGGTTCatt > 1:3205757/1‑71 (MQ=255) ttCCTGATATACGCAAGAATTCTAATCGCGTTAAGCCAATATCTAATAGTGGACGATCAACCGGGTTCatt > 1:551502/1‑71 (MQ=255) ccTGATATACGCAAGAATTCTAATCGCGTTAAGCCAATATCTAATAGTGGAc > 1:1767071/1‑52 (MQ=255) gATATACGCAAGAATTCTAATCGCGTTAAGCCAATATCTAATAGTGGACGATCAACCGGGTTCatttattt > 1:239378/1‑71 (MQ=255) gATATACGCAAGAATTCTAATCGCGTTAAGCCAATATCTAATAGTGGACGATCAACCGGGTTCatttattt > 1:670513/1‑71 (MQ=255) tataCGCAAGAATTCTAATCGCGTTAAGCCAATATCTAATAGTGGACGATCAACCGGGTTCATTTATTTc < 1:2311962/70‑1 (MQ=255) tataCGCAAGAATTCTAATCGCGTTAAGCCAATATCTAATAGTGGACGATCAACCGGGTTCATTTATTTCg < 1:3626070/71‑1 (MQ=255) taCGCAAGAATTCTAATCGCGTTAAGCCAATATCTaa < 1:3062011/37‑1 (MQ=255) taCGCAAGAATTCTAATCGCGTTAAGCCAATATCTAATAGTGGACGATCAACCGGGTTCATTTATTTCGCa > 1:2142023/1‑71 (MQ=255) cGCAAGAATTCTAATCGCGTTAAGCCAATATCTAATAGTGGACGATCAACCGGGTTCATTTATTTCGCATc > 1:3048768/1‑71 (MQ=255) cGCAAGAATTCTAATCGCGTTAAGCCAATATCTAATAGTGGACGATCAACCGGGTTCATTTATTTCGCATc > 1:3884132/1‑71 (MQ=255) cGCAAGAATTCTAATCGCGTTAAGCCAATATCTAATAGTGGACGATCAACCGGGTTCATTTATTTCGCATc < 1:677083/71‑1 (MQ=255) cAAGAATTCTAATCGCGTTAAGCCAATATCTAATAGTGGACGATCAACCGGGTTCATTTATTTCGCATCct > 1:706982/1‑71 (MQ=255) cAAGAATTCTAATCGCGTTAACCCAATATCTAATAGTGGACGATCAACCGGGTTCATTTATTTCGCATCct > 1:1110470/1‑71 (MQ=255) aGAATTCTAATCGCGTTAAGCCAATATCTAATAGTGGACGATCAACCGGGTTCATTTATTTCGCATCctct > 1:1948041/1‑71 (MQ=255) cTAATCGCGTTAAGCCAATATTTAATAGTGGACGATCAACCgg > 1:6541/1‑43 (MQ=255) cTAATCGCGTTAAGCCAATATTTAATAGTGGACGATCAACCgg > 1:4009360/1‑43 (MQ=255) cTAATCGCGTTAAGCCAATATTTAATAGTGGACGATCAACCgg > 1:865532/1‑43 (MQ=255) cTAATCGCGTTAAGCCAATATTTAATAGTGGACGATCAACCgg > 1:3494149/1‑43 (MQ=255) cTAATCGCGTTAAGCCAATATTTAATAGTGGACGATCAACCgg > 1:2926342/1‑43 (MQ=255) cTAATCGCGTTAAGCCAATATCTAATAGTGGACGATCAACCGGGTTCATTTATTTc > 1:2130272/1‑56 (MQ=255) cTAATCGCGTTAAGCCAATATCTAATAGTGGACGATCAACCGGGTTCATTTATTTc > 1:630859/1‑56 (MQ=255) tAATCGCGTTAAGCCAATATCTAATAGTGGACGATCAACCGGGTTCATTTATTTCGCATc < 1:1607966/60‑1 (MQ=255) tAATCGCGTTAAGCCAATATCTAATAGTGGACGATCAACCGGGTTCATTTATTTCGCATc < 1:2482723/60‑1 (MQ=255) aTCGCGTTAAGCCAATATCTAATAGTGGACGATCAACCGGGTTCATTTATTTCGCATCCTCTTCGCAAaca > 1:1135006/1‑71 (MQ=255) aTCGCGTTAAGCCAATATCTAATAGTGGACGATCAACCGGGTTCATTTATTTCGCATCCTCTTCGCAAaca < 1:1550976/71‑1 (MQ=255) aTCGCGTTAAGCCAATATCTAATAGTGGACGATCAACCGGGTTCATTTATTTCGCATCCTCTTCGCAAaca > 1:542832/1‑71 (MQ=255) tCGCGTTAAGCCAATATCTAATAGTGTACGATCAACCGGGTTCATTTATTTCGCATCCTCTTCGCAAacac < 1:3857598/71‑1 (MQ=255) tCGCGTTAAGCCAATATCTAATAGTGGACGATCAACCGGGTTCATTTATTTCGCATCCTCTTCGCa < 1:213364/66‑1 (MQ=255) gcgTTAAGCCAATATCTAATAGTGGACGATCAACCGGGTTCATTTATTTCGCATCctc > 1:336215/1‑58 (MQ=255) gcgTTAAGCCAATATCTAATAGTGGACGATCAACCGGGTTCATTTATTTCGCATCCTCtt > 1:4000040/1‑60 (MQ=255) gcgTTAAGCCAATATCTAATAGTGGACGATCAACCGGGTTCATTTATTTCGCATCCTCtt > 1:3802596/1‑60 (MQ=255) ttAAGCCAATATCTAATAGTGGACGATCAACCGGGTTCATTTATTTCGCa < 1:2179452/50‑1 (MQ=255) ttAAGCCAATATCTAATAGTGGACGATCAACCGGGTTCATTTATTTCGCa < 1:3632213/50‑1 (MQ=255) tAAGCCAATATCTAATAGTGGACGATCAACCGGGTTCATTTATTTCGCATCCTCTTCGCAAACACAGTGCt > 1:1810585/1‑71 (MQ=255) aaGCCAATATCTAATAGTGGACGATCAACCGGGTTCATTTATTTCGCATCCTCTTCGCaaa > 1:2146887/1‑61 (MQ=255) aaGCCAATATCTAATAGTGGACGATCAACCGGGTTCATTTATTTCGCATCCTCTTCGCaaa > 1:2202369/1‑61 (MQ=255) aaGCCAATATCTAATAGTGGACGATCAACCGGGTTCATTTATTTCGCATCCTCTTCGCaaa > 1:651830/1‑61 (MQ=255) tCTAATAGTGGACGATCAACCGGGTTCATTTATTTCGCATCCTCTTCGCaaa > 1:38898/1‑52 (MQ=255) tCTAATAGTGGACGATCAACCGGGTTCATTTATTTCGCATCCTCTTCGCAAACACAGTGCTGAc > 1:2137016/1‑64 (MQ=255) tCTAATAGTGGACGATCAACCGGGTTCATTTATTTCGCATCCTCTTCGCAAACACAGTGCTGACAATTcag > 1:4045619/1‑71 (MQ=255) tCTAATAGTGGACGATCAACCGGGTTCATTTATTTCGCATCCTCTTCGCAAACACAGTGCTGACAATTcag > 1:2498443/1‑71 (MQ=255) tCTAATAGTGGACGATCAACCGGGTTCATTTATTTCGCATCCTCTTCGCAAACACAGTGCTGACAATTcag > 1:649402/1‑71 (MQ=255) tCTAATAGTGGACGATCAACCGGGTTCATTTATTTCGCATCCTCTTCGCAAACACAGTGCTGACAATTcag > 1:3033111/1‑71 (MQ=255) tCTAATAGTGGACGATCAACCGGGTTCATTTATTTCGCATCCTCTTCGCAAACACAGTGCTGACAATTcag > 1:1574897/1‑71 (MQ=255) tCTAATAGTGGACGATCAACCGGGTTCATTTATTTCGCATCCTCTTCGCAAACACAGTGCTGACAATTcag > 1:1526855/1‑71 (MQ=255) tCTAATAGTGGACGATCAACCGGGTTCATTTATTTCGCATCCTCTTCGCAAACACAGTGCTGACAATTca > 1:451767/1‑70 (MQ=255) tCTAATAGTGGACGATCAACCGGGTTAatttattt > 1:2700722/1‑35 (MQ=255) cTAATAGTGGACGATCAACCGGGTTCATTTATTTCGCATCCTCTTCGCAAacac < 1:3858061/54‑1 (MQ=255) | GGGCAATTGTTAGACCAGCAAGGCCTTTTCCTGATATACGCAAGAATTCTAATCGCGTTAAGCCAATATCTAATAGTGGACGATCAACCGGGTTCATTTATTTCGCATCCTCTTCGCAAACACAGTGCTGACAATTCAG > minE/1082072‑1082210 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |