| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,094,567 | T→C | 33.3% | I604V (ATT→GTT) | ydiJ ← | predicted FAD‑linked oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,094,567 | 0 | T | C | 33.3% | 62.4 / 52.4 | 57 | I604V (ATT→GTT) | ydiJ | predicted FAD‑linked oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base T (20/18); new base C (10/9); total (30/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
GCCGCGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGT > minE/1094502‑1094635 | gccgcGATCCGCCAACAAACGCAGCCATTCACGCCCCAGCGTTGCGCGCCCTTTCGGTGAATGAACCCGGt > 1:3110066/1‑71 (MQ=255)gccgcGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAACCCGGt > 1:3010894/1‑71 (MQ=255)gccgcGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAACCCGGt > 1:3442040/1‑71 (MQ=255) cgcgATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTc < 1:3589212/71‑1 (MQ=255) cgcgATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTc < 1:2244312/71‑1 (MQ=255) cgcgATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTc < 1:3794971/71‑1 (MQ=255) cgcgATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTc < 1:2121667/71‑1 (MQ=255) ccGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAACCCGGTTCTGGGt > 1:1474944/1‑70 (MQ=255) cGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAACCCGGTTCTGGGTg < 1:1300538/70‑1 (MQ=255) cGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAACCCGGTTCTGGGTg < 1:3778496/70‑1 (MQ=255) aaaCGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAACCCGGTTCTGGGTGATCTTCAt > 1:2952103/1‑71 (MQ=255) aaaCGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAACCCGGTTCTGGGTGATCTTCAt > 1:2285726/1‑71 (MQ=255) aaaCGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAACCCGGTTCTGGGTGATCTTCAt > 1:2143816/1‑71 (MQ=255) aaaCGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAACCCGGTTCTGGGTGATCTTCAt > 1:1487982/1‑71 (MQ=255) aaCGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATc < 1:769142/71‑1 (MQ=255) aaCGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATc < 1:1838316/71‑1 (MQ=255) gCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAACCCGGTTCTGGGTg < 1:3124679/59‑1 (MQ=255) gCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGa < 1:3821289/71‑1 (MQ=255) gCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGa < 1:4093009/71‑1 (MQ=255) cATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGaca < 1:3592776/71‑1 (MQ=255) tCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACAt > 1:4013628/1‑71 (MQ=255) tCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACAt > 1:2711837/1‑71 (MQ=255) cACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATc > 1:3791646/1‑71 (MQ=255) cACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATc > 1:2252052/1‑71 (MQ=255) cACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATc > 1:2748763/1‑71 (MQ=255) aCGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCg < 1:1640670/71‑1 (MQ=255) aCGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCg < 1:1044575/71‑1 (MQ=255) aCGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAACCCGGTTCTGGGTGATCTTCATCGACGGACACATCg < 1:2361188/71‑1 (MQ=255) gCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCgg < 1:1892313/70‑1 (MQ=255) cAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTAc < 1:995342/71‑1 (MQ=255) cAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTAc < 1:881642/71‑1 (MQ=255) cAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTAc < 1:386748/71‑1 (MQ=255) aGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACg > 1:547268/1‑71 (MQ=255) aGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACg > 1:3070344/1‑71 (MQ=255) aGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACg > 1:4098458/1‑71 (MQ=255) gCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTc > 1:47764/1‑47 (MQ=255) tGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCAt > 1:1403616/1‑71 (MQ=255) gcgcgcCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCgg > 1:2279447/1‑59 (MQ=255) gcgcgcCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGa > 1:3682355/1‑60 (MQ=255) gcgcgcCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGa > 1:2473337/1‑60 (MQ=255) gcgcgcCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGa > 1:2200209/1‑60 (MQ=255) cgcgcCCTTTCTGTGAATGAACCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCa < 1:3936564/71‑1 (MQ=255) cgcgcCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATc < 1:2543157/70‑1 (MQ=255) cgcgcCCTTTCGGTGAATGAACCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCa < 1:1350096/71‑1 (MQ=255) cgcgcCCTTTCGGTGAATGAACCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCa < 1:893668/71‑1 (MQ=255) cgcCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCaaa < 1:693901/71‑1 (MQ=255) cgcCCTTTCGGTGAATGAACCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCAtaa > 1:1624598/1‑67 (MQ=255) cTTTCGGTGAATGAACCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTg < 1:3064006/71‑1 (MQ=255) cTTTCGGTGAATGAACCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTg < 1:586221/71‑1 (MQ=255) tCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTa > 1:3630044/1‑53 (MQ=255) tCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGaa > 1:1157582/1‑70 (MQ=255) tCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAg > 1:390214/1‑71 (MQ=255) tCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAg > 1:3425914/1‑71 (MQ=255) tCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAg > 1:293875/1‑71 (MQ=255) tCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAg > 1:2122327/1‑71 (MQ=255) tCGGTGAATGAACCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAg > 1:108809/1‑71 (MQ=255) ggTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGc < 1:3390226/70‑1 (MQ=255) aatgaatCCGGTTCTGGGTGATCTTCATCGACGGACACATCg < 1:4125754/42‑1 (MQ=255) aatgaatCCGGTTCTGGGTGAGCTTCATCGACGGACACATCg < 1:385002/42‑1 (MQ=255) aatCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGt < 1:2370766/71‑1 (MQ=255) aatCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGt < 1:2126987/71‑1 (MQ=255) aatCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGt < 1:1991017/71‑1 (MQ=255) | GCCGCGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGT > minE/1094502‑1094635 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |