| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,111,527 | G→A | 21.6% | C287Y (TGT→TAT) | ydiD → | short chain acyl‑CoA synthetase, anaerobic |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,111,527 | 0 | G | A | 21.6% | 107.9 / 25.1 | 51 | C287Y (TGT→TAT) | ydiD | short chain acyl‑CoA synthetase, anaerobic |
| Reads supporting (aligned to +/- strand): ref base G (19/21); new base A (5/6); total (24/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01 | |||||||||||
TAACGGCACCATTCTTAATTGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTGTCTCGCGCTGCTTGAGCAGCAGCGTTGCACCTGTATGCTCGGCGCAACGCCGTTTGTCTATGATCT > minE/1111464‑1111593 | tAACGGCACCATTCTTAATTGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTGtctc < 1:1027878/68‑1 (MQ=255)tAACGGCACCATTCTTAATTGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTGtctc < 1:82632/68‑1 (MQ=255)tAACGGCACCATTCTTAATTGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTGtctc < 1:146123/68‑1 (MQ=255)tAACGGCACCATTCTTAATTGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTGtctc < 1:363530/68‑1 (MQ=255)tAACGGCACCATTCTTAATTGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTGtctc < 1:2868158/68‑1 (MQ=255)tAACGGCACCATTCTTAATTGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTGtctc < 1:2026555/68‑1 (MQ=255) ccATTCTTAATTGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTGTCTcgcg < 1:2463168/63‑1 (MQ=255) ccATTCTTAATTGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCtt < 1:1536126/69‑1 (MQ=255) ccATTCTTAATTGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCAGATGCGTGTCTcgcg < 1:1899772/63‑1 (MQ=255) aTTCTTAATTGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGa > 1:3604630/1‑69 (MQ=255) tCTTAATTGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTGTCTCGCGCTGCTTGagcag > 1:710766/1‑71 (MQ=255) tCTTAATTGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTGTCTCGCGCTGCTTGagc > 1:97594/1‑69 (MQ=255) tCTTAATTGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTGTCTCGCGCTGCTTGagc > 1:477396/1‑69 (MQ=255) tCTTAATTGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTGTCTCGCGCTGCTTGagc > 1:2942069/1‑69 (MQ=255) tCTTAATTGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTGTCTCGCGCTGCTTGagc > 1:2268834/1‑69 (MQ=255) cTTAATTGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTGTCTCGCGCTGCTTGagcag > 1:3652417/1‑70 (MQ=255) cTTAATTGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagcag < 1:3525118/70‑1 (MQ=255) cTTAATTGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagcag < 1:1261346/70‑1 (MQ=255) cTTAATTGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagcag < 1:1519331/70‑1 (MQ=255) cTTAATTGGCGCCCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagcag < 1:3704128/70‑1 (MQ=255) aaTTGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTGTCTCGCGCTGCTTGagcagcag < 1:2377803/70‑1 (MQ=255) aaTTGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTGTCTCGCGCTGCTTGagcagcag < 1:2544078/70‑1 (MQ=255) aaTTGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTGTCTCGCGCTGCTTGagcagc < 1:3273906/68‑1 (MQ=255) gCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTGTCTCGCGCTGCTTGAGCAGCAGCGTTGCACCTg < 1:3181339/70‑1 (MQ=255) gCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTGTCTCGCGCTGCTTGAGCAGCAGCGTTGCACCTg < 1:620573/70‑1 (MQ=255) gCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTGTCTCGCGCTGCTT‑AGCAGCAGCGTTGCACCTg < 1:1080983/69‑1 (MQ=255) tgttAGATATTTTCACTCCTGATGCGTGTCTCGCGCTGCTTGAGCAGCAGCGTTGCACCTGTATGCTCGgc > 1:2193438/1‑71 (MQ=255) gttAGATATTTTCACTCCTGATGCGTGTCTCGCGCTGCTTGAGCAGCAGCGTTGCACCTGTATGCTCGgcg > 1:792876/1‑71 (MQ=255) atTTTCACTCCTGATGCGTGTCTCGCGCTGCTTGagcag > 1:4057097/1‑39 (MQ=255) atTTTCACTCCTGATGCGTGTCTCGCGCTGCTTGagcag > 1:805828/1‑39 (MQ=255) atTTTCACTCCTGATGCGTGTCTCGCGCTGCTTGagcag > 1:3450550/1‑39 (MQ=255) atTTTCACTCCTGATGCGTGTCTCGCGCTGCTTGagcag > 1:2180993/1‑39 (MQ=255) atTTTCACTCCTGATGCGTGTCTCGCGCTGCTTGagcag > 1:1999345/1‑39 (MQ=255) atTTTCACTCCTGATGCGTGTCTCGCGCTGCTTGAGCAGCAGCGTTGCACCTGTATGCTCGGCGCAACGcc > 1:3320408/1‑71 (MQ=255) atTTTCACTCCTGATGCGTGTCTCGCGCTGCTTGAGCAGCAGCGTTGCACCTGTATGCTCGGCGCAACGcc > 1:1557539/1‑71 (MQ=255) tttCACTCCTGATGCGTGTCTCGCGCTGCTTGAGCAGCAGCGTTGCACCTGTATGCTCGGCGCAACGCCGt > 1:2253229/1‑71 (MQ=255) tttCACTCCTGATGCGTATCTCGCGCTGCTTGAGCAGCAGCGTTGCACCTGTATGCTCGGCGCAACGCCGt > 1:324690/1‑71 (MQ=255) tttCACTCCTGATGCGTATCTCGCGCTGCTTGAGCAGCAGCGTTGCACCTGTATGCTCGGCGCAACGCCGt > 1:2230031/1‑71 (MQ=255) tttCACTCCTGATGCGTATCTCGCGCTGCTTGAGCAGCAGCGTTGCACCTGTATGCTCGGCGCAACGCCGt > 1:3090007/1‑71 (MQ=255) tttCACTCCTGATGCGTATCTCGCGCTGCTTGAGCAGCAGCGTTGCACCTGTATGCTCGGCGCAACGCCGt > 1:4109996/1‑71 (MQ=255) ttCACTCCTGATGCGTGTCTCGCGCTGCTTGAGCAGCAGCGTTGCACCTGTATGCTCGGCGCAACGCCGtt > 1:20340/1‑71 (MQ=255) ttCACTCCTGATGCGTATCTCGCGCTGCTTGAGCAGCAGCGTTGCACCTGTATGCTCGGCGCAACGCCGt < 1:2346892/70‑1 (MQ=255) ccTGATGCGTGTCTCGCGCTGCTTGAGCAGCAGCGTTGCAc > 1:3291423/1‑41 (MQ=255) ccTGATGCGTGTCTCGCGCTGCTTGAGCAGCAGCGTTGCAc > 1:665974/1‑41 (MQ=255) aTGCGTGTCTCGCGCTGCTTGAGCAGCAGCGTTGCACCTGTATGCTCGGCGCAACGGCGTTTGTCTATg < 1:719771/69‑1 (MQ=255) aTGCGTGTCTCGCGCTGCTTGAGCAGCAGCGTTGCACCTGTATGCTCGGCGCAACGCCGTTTGTCTa < 1:2184815/67‑1 (MQ=255) aTGCGTGTCTCGCGCTGCTTGAGCAGCAGCGTTGCACCTGTATGCTCGGCGCAACGCCGTTTGTCTATg < 1:1790030/69‑1 (MQ=255) aTGCGTGTCTCGCGCTGCTTGAGCAGCAGCGTTGCACCTGTATGCTCGGCGCAACGCCGTTTGTCTATg < 1:1752845/69‑1 (MQ=255) aTGCGTGTCTCGCGCTGCTTGAGCAGCAGCGTTGCACCTGTATGCTCGGCGCAACGCCGTTTGTCTATg < 1:1625018/69‑1 (MQ=255) cGTGTCTCGCGCTGCTTGAGCAGCAGCGTTGCACCTGTATGCTCGGCGCAACGCCGTTTGTCTATGATCt < 1:553392/70‑1 (MQ=255) cGTGTCTCGCGCTGCTTGAGCAGCAGCGTTGCACCTGTATGCTCGGCGCAACGCCGTTTGTCTATGATCt < 1:1446339/70‑1 (MQ=255) | TAACGGCACCATTCTTAATTGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTGTCTCGCGCTGCTTGAGCAGCAGCGTTGCACCTGTATGCTCGGCGCAACGCCGTTTGTCTATGATCT > minE/1111464‑1111593 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |